Flowering regulating gene AcFT and application thereof

A technology for regulating genes and genes, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve problems such as delay in flowering time of rice

Inactive Publication Date: 2012-01-04
SOUTH SUBTROPICAL CROPS RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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Problems solved by technology

When the expression level of Hd3α was down-regulated by RNA i

Method used

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  • Flowering regulating gene AcFT and application thereof
  • Flowering regulating gene AcFT and application thereof
  • Flowering regulating gene AcFT and application thereof

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Experimental program
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Embodiment Construction

[0018] The present invention will be described in further detail below in conjunction with specific examples, and these examples are only used to illustrate the present invention, and do not limit the scope of the present invention.

[0019] The FT homologous gene provided by the present invention is cloned from pineapple, named AcFT gene, and its nucleic acid sequence is as shown in SEQ ID NO: 1 (sequence 1).

[0020] The protein encoded by the AcFT gene is named as AcFT protein, and its amino acid sequence is shown in SEQ ID NO: 2 (SEQ ID NO: 2).

[0021] 1. Cloning and sequence analysis of AcFT gene

[0022] The above-mentioned AcFT gene cloning method and its sequence analysis comprise the following steps:

[0023] 1. RNA extraction

[0024] Using the apical meristem of the pineapple variety "Bali" as the material, the total RNA was extracted with the column plant RNAout 2.0 kit (product number: CAT#: 90404-50) of Beijing Tianenze Gene Technology Co., Ltd. The specific o...

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Abstract

The invention relates to a flowering regulating gene AcFT obtained from pineapple by cloning and application thereof in flowering regulation of arabidopsis thaliana. The nucleotide sequence of the AcFT gene is as shown in SEQ ID No. 1 (sequence identity number 1) and the sequence of amino acid encoded by the AcFT gene is as shown in SEQ ID No. 2. The AcFT gene is obtained by cloning through the homologous sequence method, the arabidopsis thaliana is transformed by adopting the pollen tube channel method, and the results prove that a transgenic arabidopsis thaliana plant flowers in advance in comparison with a wild type arabidopsis thaliana plant. The invention is conductive to further understanding the flowering mechanism of pineapple so that the regulation of the flowering phase of pineapple and breeding of new varieties of pineapple can be realized by using a biotechnical means.

Description

technical field [0001] The invention relates to a flowering regulation gene AcFT cloned from pineapple and its application in Arabidopsis flowering regulation. Background technique [0002] Pineapple (Ananas comosus) is a perennial herb of the order Gramineae, Bromeliaceae, and the genus Bromeliad, and it is also one of the famous tropical and subtropical fruits. Its fruit is rich in various sugars, organic acids, mineral elements and multivitamins necessary for the human body. It has high nutritional value and can be eaten fresh and processed. The processed skin dregs can be used as feed and fertilizer, and high-quality fiber can be extracted from pineapple leaves. . Pineapples are widely used and have broad markets and development prospects at home and abroad. According to FAO statistics, in 2009, my country's pineapple output was 1.45 million tons, making it the third largest pineapple producer in the world after Thailand and the Philippines. my country has introduced ...

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Application Information

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IPC IPC(8): C12N15/29C12N15/82C12N5/10C07K14/415A01H5/00
Inventor 吕玲玲孙光明段俊谢江辉陈长明刘玉革刘胜辉魏长宾张建霞
Owner SOUTH SUBTROPICAL CROPS RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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