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Biomarker reagent used for detecting breast cancer state

A biomarker, breast cancer technology, applied in the direction of testing material hardness, measuring devices, instruments, etc., can solve the problems of recurrence and metastasis, high fatality rate, poor prognosis, etc., and achieve the effect of accurate prediction

Inactive Publication Date: 2012-01-04
SUZHOU UNIV
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Problems solved by technology

[0002] Breast cancer is a common malignant tumor in women. More than 30% of patients die of breast cancer recurrence and metastasis every year. Breast cancer metastasis is one of the main reasons for the failure of breast cancer treatment. Once breast cancer metastasizes, even surgical treatment, It will also seriously affect the quality of life of patients, and 1 / 3 of breast cancer patients are at risk of recurrence and metastasis, resulting in poor prognosis and high mortality

Method used

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  • Biomarker reagent used for detecting breast cancer state
  • Biomarker reagent used for detecting breast cancer state
  • Biomarker reagent used for detecting breast cancer state

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Embodiment 1

[0018] 1.1 Materials: Human breast cancer cell lines MDA-MB-231 and MDA-MB-453 were purchased from the American Cell Collection Center (ATCC), and preserved and cultivated by our laboratory; D-MEM medium dry powder, calf serum, trypsin The powder was purchased from Gibco; standard protein molecular weight, SDS-PAGE loading buffer, RIPA protein lysate, TE electrophoresis buffer, 10× transfer solution, 30% Acry-Bis, Tris-Hcl, ammonium persulfate (AP) , SDS, tetramethylethylenediamine (TEMED), and propidium iodide (PI) were purchased from Jiangsu Beyantian Biotechnology Research Institute; dimethyl sulfoxide (DMSO), agarose, and Tween-20 were purchased from Shanghai High-Tech Biology Engineering Co., Ltd.; anti-UHRF1, cyclin B1, cyclin D1 and other antibodies were purchased from Santa Cruzs Biotechnology Company, USA. RNase A and 10 μg / mL proteinase K were purchased from Sigma Aldrich, USA.

[0019] 1.2 Cell culture: MDA-MB-231 and MDA-MB-453 cells were cultured in D-EME medium ...

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Abstract

The invention discloses a biomarker reagent used for detecting a breast cancer state, which is an ultra high resolution fracsimile (UHRF 1) gene. The UHRF 1 gene is closely related to distant metastasis capacity of a breast cancer; the UHRF 1 gene is used as a biomarker predicting the distant metastasis capacity of the breast cancer, and the malignancy degree of the breast cancer can be predicted more accurately in a more individualized manner compared with transferring and prognosis of the breast cancer judged by the conventional pathological type.

Description

technical field [0001] The present invention relates to a biomarker reagent for detecting the status of breast cancer. Background technique [0002] Breast cancer is a common malignant tumor in women. More than 30% of patients die of breast cancer recurrence and metastasis every year. Breast cancer metastasis is one of the main reasons for the failure of breast cancer treatment. Once breast cancer metastasizes, even surgical treatment, It will also seriously affect the quality of life of patients, and 1 / 3 of breast cancer patients are at risk of recurrence and metastasis, resulting in poor prognosis and high mortality. [0003] Metastasis of breast cancer is related to its own biological characteristics and host environment and gene changes. It is a complex process involving multiple genes and multiple steps. Degradation and destruction of extracellular matrix and basement membrane by cells, cell movement and migration caused by cytoskeleton remodeling, organ-specific homin...

Claims

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Application Information

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IPC IPC(8): G01N3/50G01N33/574
Inventor 李新莉樊赛军
Owner SUZHOU UNIV
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