Selenium-containing unicellular microalgae for animal plankton feeds and method of culturing selenium-containing animal planktons using the same

A zooplankton, single-cell technology, applied in the field of preventing selenium deficiency in larvae and juveniles during post-hatch cultivation during the production of fish seedlings, and can solve the problems of rotifers and unknown effects of hatching larvae and juveniles.

Inactive Publication Date: 2012-01-04
CHLORELLA IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, so far there is no research and development on fortifying zooplankto...

Method used

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  • Selenium-containing unicellular microalgae for animal plankton feeds and method of culturing selenium-containing animal planktons using the same
  • Selenium-containing unicellular microalgae for animal plankton feeds and method of culturing selenium-containing animal planktons using the same
  • Selenium-containing unicellular microalgae for animal plankton feeds and method of culturing selenium-containing animal planktons using the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Production of selenium-containing rotifers using selenium-containing live chlorella as bait

[0046] A 10L fermenter was used for the cultivation of selenium-containing live Chlorella. A slant of a Chlorella vulgaris strain owned by our company was aseptically inoculated into a Sakaguchi flask of a dark culture basal medium, and preculture was performed at 28°C. After the assimilation of glucose was completed, the inoculation was continued in the 10L culture tank, and the aseptic culture of selenium-fortified Chlorella was carried out. The culture conditions are as follows: culture medium volume 6L, temperature 36°C, ventilation 3L / min, pH 7.0, stirring speed 300rpm / min. The composition of the medium used is glucose 80g / L, potassium phosphate 2.0g / L, magnesium sulfate 2.0g / L, Fe·EDTA 4.8mg / L, trace mineral A 5 (×10)1.08ml / L, Vitamin B 12 Add 5.4μg / L, 18μg / L, 54μg / L, 180μg / L and 540μg / L sodium selenite to the basal medium of 2.4mg / L and urea 6.0g / L respectively, and ...

Embodiment 2

[0054] Production of selenium-containing Daphnia using live selenium-containing Nannochloropsis as bait

[0055] The culture of Nannochloropsis containing selenium was carried out using a plate culture tank with a volume of 30 L. From the Nannochloropsis slant held by our company, carry out aseptic inoculation in a 100ml volume small flat bottle containing the bright culture basal medium, in 80ml of the bright culture basal medium, 5% (v / v) CO 2 Two weeks of pre-cultivation were carried out under the conditions of ventilation and stirring, illuminance of 2000 lux, and water temperature of 20°C. When the concentration of algae reached 2g / L (relative to the dry matter), inoculation was continued in the 30L plate culture tank to carry out selenium-enhanced cultivation. The culture conditions are as follows: medium volume 25L, 5% CO 2 Ventilation and stirring 3L / min, illumination 2500 lux, water temperature 20°C. The composition of the medium is potassium nitrate 0.5g / L, sodiu...

Embodiment 3

[0058] Feeding experiment of flounder larvae using selenium-containing rotifers

[0059] A rearing experiment of flounder (Paralichthys olivacens) larvae was carried out using selenium-containing rotifers produced with selenium-containing live chlorella as bait. The breeding conditions are as follows: put 500L of filtered seawater into each 600L circular polycarbonate water tank, the water exchange rate is 100% / day, the water temperature is 17-20°C, the ventilation is 500ml / min, and it accommodates 1.1×10 4 Tail hatching larvae (body length 2.1±0.05mm *1 ). As the test bait, use selenium-containing rotifers (the selenium content is 100 μg / 100g dry weight) to start raising, feed twice a day so that the rotifers density in the breeding water is 6-12 individuals / ml, in a total of 2 water tanks (a In , b), a 17-day feeding test was carried out. As a result, it was observed that the flounder larvae were fed vigorously and grew smoothly during the test period.

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Abstract

Based on the finding that no or very little selenium is detected in animal planktons which are cultured by the existing method, it is noticed that there is a possibility that marine larval fish at the stage of taking these biological feeds would suffer from selenium deficiency. Selenium-containing animal planktons can be successfully produced by developing a method of producing selenium-containing animal planktons, preliminarily incorporating selenium into unicellular microalgae, and culturing animal planktons with the use of the unicellular microalgae as a feed. Thus, the survival rate and vitality of larval fish having been deficient in selenium can be remarkably elevated.

Description

technical field [0001] The present invention relates to a method for preventing selenium deficiency in larvae during post-hatch cultivation during the production of fish seedlings. Specifically, the present invention relates to a zooplankton culture bait (ie, selenium-containing unicellular microalgae) used as a bait for juvenile fish, and a method for cultivating selenium-containing zooplankton using the selenium-containing unicellular microalgae. Background technique [0002] Fish farming has increased in recent years as fish stocks have dwindled worldwide and health awareness has increased. There are roughly two farming methods: the method of catching natural juvenile fish and feeding them with mixed feed and raising them to a marketable size, and the method of taking eggs from broodstock and raising them after hatching. In the latter case, juveniles grow on nutrients called yolk for a certain period of time after hatching, and then open their mouths and start feeding, b...

Claims

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Application Information

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IPC IPC(8): A23K1/18A01K61/00A23K1/175A01G33/00A01K61/13C12N1/12
CPCA01K61/00A23K1/1758A23K1/188A23K20/30A23K50/80Y02A40/81A01K61/20
Inventor 丸山功中村寿雄古贺直树北岛佳孝安藤洋太郎
Owner CHLORELLA IND
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