Method for extracting human chorionic gonadotropin (HCG)

A chorionic gonadotropic and alkaline technology, which is applied in the field of chemical drug extraction technology, can solve the problems of cumbersome process steps, low yield, and large amount of manual operation

Inactive Publication Date: 2012-01-11

SHANDONG TOPSCI BIO TECH

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AI-Extracted Technical Summary

Problems solved by technology

[0002] Human chorionic gonadotropin is a glycoprotein secreted by the trophoblast cells of the placenta. It is composed of α and β dimer glycoproteins. It is clinically used for functional uterine bleeding, male cryptorchidism, hypogonadism and other diseases , is a commonly used drug with definite curative effect and wide application, so the method of extr...

Method used

2, because the ultrafiltration membrane that designed molecular weight is 10000 carries out ultrafiltration, removes the impurity in pregnant woman's urine, has guaranteed the quality of human chorionic gonadotropin.

3, because the organic solvent that the composition that content is 95% is ethanol has been designed, the extraction process flow of human chorionic gonadotropin is simplified, manual operation is reduced...

Abstract

Belonging to the technological field of chemical drug extraction, the invention discloses a method for extracting human chorionic gonadotropin (HCG). The method comprises the steps of: adjusting the pH value of pregnant woman urine to alkaline, conducting filtration and ultrafiltration so as to obtain concentrated urea, then adding an organic solvent, and leaving the mixture to stand, so that a precipitate can be obtained, and drying the precipitate, thus obtaining the HCG. The method of the invention has the advantages of simplified technology, high product yield, low production cost, and the like.

Application Domain

DepsipeptidesPeptide preparation methods

Technology Topic

DrugUltrafiltration +7

Examples

Experimental program(1)

Example Embodiment

[0008] Embodiments of the present invention, the steps are: extract from the urine of pregnant women, add 20% sodium hydroxide to the urine of pregnant women, adjust the pH of the urine of pregnant women to 10.0-10.5, filter through filter paper, and obtain by ultrafiltration with an ultrafiltration machine Concentrate the urine, then add an organic solvent with a content of 95% ethanol, let it stand for 10-16 hours to obtain a precipitate, and dry the precipitate for 8 hours at a temperature of 22-28°C to obtain human chorion gonadotropins.

[0009] In the production of human chorionic gonadotropin in our company, the benzoic acid adsorption method was used before, and only about 40 grams of human chorionic gonadotropin could be extracted per 100 kilograms of pregnant women’s urine. Now about 60 grams of human chorionic gonadotropin can be extracted from every 100 kilograms of pregnant women's urine, thus increasing the yield.

[0010] The present invention has the following characteristics:

[0011] 1. Due to the design of an organic solvent with a content of 95% ethanol, the human chorionic gonadotropin in the urine of pregnant women can be fully extracted, thereby increasing the output of human chorionic gonadotropin.

[0012] 2. Due to the design of the ultrafiltration membrane with a molecular weight of 10,000 for ultrafiltration, impurities in the urine of pregnant women are removed, and the quality of human chorionic gonadotropin is guaranteed.

[0013] 3. Due to the design of an organic solvent with a content of 95% ethanol, the extraction process of human chorionic gonadotropin is simplified, manual operations are reduced, production efficiency is improved, the production cycle is shortened to 2 days, and the yield of the product is improved. It is also increased from the traditional 40-60% to more than 90%.

[0014] In the technical field of the method for extracting human chorionic gonadotropin; the steps are: extracting from the urine of pregnant women, adjusting the pH of the urine of pregnant women to 10.0-10.5, and then adding an organic solvent with a content of 95% as ethanol, After standing still for 10-16 hours, a precipitate is obtained, and the technical content of drying the precipitate in an environment with a temperature of 22-28° C. is within the protection scope of the present invention.

PUM

Description & Claims & Application Information

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