Serum-free medium for culturing hepatic cells

Abstract

The invention discloses a serum-free medium for culturing hepatic cells. The serum-free medium comprises a basic medium and additive components, wherein, the additive components comprise 0.1 to 10 mu g / mL of insulin, 0.5 to 10 mu g / mL of transferrin, 5 to 10 mu g / mL of sodium selenite, 1 to 100 ng / mL of epidermal growth factors, 1 to 100 ng / mL of hepatocyte growth factors, 0.1 to 1 mu g / mL of fibronectin, 0.1 to 10 nmol / mL of dexamethasone and 0.05 to 5 mu g / mL of glucagon. The serum-free medium provided in the invention does not contain fetal calf serum or any animal origin components and supplies sufficient nutrition and a good environment needed for growth and increment of cells; all the additive components can effectively substitute serum components through a variety of mechanisms in the process of culturing hepatic cells; the cells have good growth, and the morphology, density, vitality and functions of the cells are basically the same as those of cells in a serum-containing medium.

Description

technical field [0001] The invention relates to a medium for cell culture, in particular to a serum-free medium for culturing hepatocytes. Background technique [0002] As one of the most important organs in the human body, the liver undertakes various complex functions such as synthesis, secretion, metabolism, and detoxification. Once a large number of necrosis of liver cells are caused by various reasons, liver failure will occur, resulting in metabolic disorders and accumulation of toxic substances, which in turn aggravates liver cell damage and affects liver cell regeneration, resulting in liver failure. vicious circle. Acute liver failure is a serious liver disease with a mortality rate as high as 60-90%. Currently, the most effective treatment for this is liver transplantation. However, due to the lack of donor organs, high cost, and long-term use of immunosuppressive agents, patients often die rapidly due to disease progression while waiting for a donor, which grea...

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Problems solved by technology

[0004] However, research has found that there are many disadvantages in the use of serum in cell culture: first, there are batch-to-batch differences, and the biological activities and factors between different batches of serum are inconsistent, which will lead to poor reproducibility of products and experimental results, and require a large number of Verification work; secondly, the source of serum is unstable, its components are unclear, and it has growth-inhibiting components, which is not conducive to the separation and purification of target products such as vaccines and monoclonal antibodies; moreover, there is a risk of contamination of exogenous viruses and pathogenic factors , easy to be infected by viruses and mycoplasma; furthermore, the residual bovine serum in the product is likely to cause allergic reactions to the serum in the inoculated person, which is not conducive to animal experiments or clinical trials.

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