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PH self-adjusting double-response hydrogel as well as synthesis method and application thereof

A dual-response, hydrogel technology, applied in the fields of pharmaceutical formulations, medical science, bandages, etc., to achieve the effect of simple and easy preparation method, reducing ROS (reactive oxygen) concentration, and lowering blood sugar level

Pending Publication Date: 2022-02-08
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Their research points generally focus on the effects of active substances on cell migration and proliferation, blood vessel and tissue regeneration, and wound healing, but there are few reports on the regulation of dressings on the wound microenvironment and its impact on wound healing

Method used

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  • PH self-adjusting double-response hydrogel as well as synthesis method and application thereof
  • PH self-adjusting double-response hydrogel as well as synthesis method and application thereof
  • PH self-adjusting double-response hydrogel as well as synthesis method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Preparation of glucose oxidase grafted chitosan / oligomeric proanthocyanidins / halloysite nanotubes (CS-GOX / OPC / HNTs) composite hydrogel:

[0074] (1) Preparation of CS-GOX

[0075] Weigh 320mg of CS and dissolve in 320mL of PBS (pH6.0), stir and dissolve at room temperature, then add dropwise 0.19mg of SPDP solution (solvent is DMSO) and stir for 1-3 hours to obtain the CS solution; dissolve 40mg of GOX, dissolve GOX solution was obtained in 40 mL of PBS (pH 6.0); GOX solution was added to CS solution, and after stirring at room temperature for 24 h, the reaction solution was transferred to a dialysis bag with a molecular weight cut-off of 14 kDa, and dialyzed with deionized water for 3 days. The deionized water was replaced once every 4 hours, and then the retentate was collected and freeze-dried to obtain a light yellow product glucose oxidase grafted to chitosan (CS-GOX).

[0076] (2) Weigh a certain amount of CS-GOX, add deionized water to configure a CS-GOX solutio...

Embodiment 2

[0078] Preparation of chitosan / oxidized dextran / halloysite nanotubes (CS / Oxd / HNTs) double-loaded hydrogel

[0079] (1) Dissolve 20 mg of CS in 1 mL of deionized water, stir and dissolve at room temperature, adjust the pH value to 7.4, and obtain a CS solution with a concentration of 20 mg / mL, and then add a certain amount of insulin to the CS solution (the final concentration of insulin 6 mg / mL) and GOX (the final concentration of GOX is 6 mg / mL), stirred to fully dissolve, and mixed solution A was obtained.

[0080] (2) Weigh a certain amount of oxidized dextran (Oxd), add sterile deionized water to prepare an Oxd solution with a concentration of 15 mg / mL, and adjust the pH value to 8.

[0081] (3) Weigh a certain amount of halloysite nanotubes, add them into the Oxd solution, stir to make them uniformly dispersed, so that the concentration of halloysite nanotubes is 10 mg / mL, and mixed solution B is obtained.

[0082] (4) Collect L929 cells in good growth state, disperse them...

Embodiment 3

[0085] (1) Dissolve 40 mg of CS in 1 mL of deionized water, stir and dissolve at room temperature, adjust the pH value to 7.4, and obtain a CS solution with a concentration of 40 mg / mL, and then add a certain amount of insulin to the CS solution (the final concentration of insulin 6 mg / mL) and GOX (the final concentration of GOX is 4 mg / mL), stirred to fully dissolve to obtain mixed solution A.

[0086] (2) Weigh a certain amount of oxidized dextran (Oxd), add sterile deionized water to prepare an Oxd solution with a concentration of 40 mg / mL, and adjust the pH value to 8.

[0087] (3) Weigh a certain amount of halloysite nanotubes, add them into the Oxd solution, stir to make them uniformly dispersed, so that the concentration of halloysite nanotubes is 0.4 mg / mL, and mixed solution B is obtained.

[0088] (4) Mix the mixed solution A with the mixed solution B at a volume ratio of 1:1, stir rapidly to make it fully mixed, and form a hydrogel after standing for about 5 minutes...

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Abstract

The invention discloses pH self-adjusting double-response hydrogel as well as a synthesis method and application thereof, and belongs to the technical field of preparation of diabetic wound dressings. The pH self-adjusting double-response hydrogel is prepared from the following raw materials: a high polymer material containing amino and hydroxyl functional groups, a high polymer material containing aldehyde groups and hydroxyl functional groups, a nano material with hydroxyl functional groups on the surface, glucose oxidase and a medicine. The pH self-adjusting double-response hydrogel prepared by the invention has a pH self-adjusting capability, can reduce the environmental pH from the physiological pH (7.4) and maintain the environmental pH in a weakly acidic range with antibacterial activity, reduces the blood sugar level and the ROS (reactive oxygen species) concentration, and promotes the healing of chronic wounds; and meanwhile, the hydrogel encapsulates drugs and cells and continuously and controllably releases the drugs, the cell growth state is good, and the hydrogel is ideal target hydrogel.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a pH self-adjusting dual-response hydrogel and its synthesis method and application. Background technique [0002] Ulcer in type 1 and type 2 diabetes is one of the most difficult clinical complications and has become an urgent medical, social and economic problem worldwide. Normally, wound healing can generally be divided into several partially overlapping phases, including hemostasis, inflammation, hyperplasia, and tissue remodeling. Acute wounds usually go through these stages on a regular basis, eventually healing within a few weeks. The healing process of diabetic ulcers is more complex. Due to the high blood sugar in the patient's body, it will cause neuropathy and peripheral vascular disease, which will hinder the healing process at a certain stage and form a chronic wound. Due to the continuous existence of tissue trauma, a large number of inflammatory cells flood i...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08L5/08C08L5/02C08L71/02C08K7/26A61L26/00
CPCC08J3/075A61L26/008A61L26/0061A61L26/0066A61L26/0057A61L26/0004A61L26/0023A61L26/0019C08J2305/08C08J2305/02C08J2371/02C08K7/26A61L2400/06A61L2300/404A61L2300/602A61L2300/254A61L2400/12A61L2300/252C08L5/08C08L5/02C08L71/02Y02A50/30
Inventor 赵玲玲张佳颖崔明占陈凤娇戴范佳张聃梁洪泽
Owner NINGBO UNIV
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