Analysis method of citric acid fermentation aqueous solution

A technology of fermented clear liquid and analysis method, which is applied in the field of analysis of citric acid fermented clear liquid, can solve the problems of inability to accurately quantify the content of various sugars, and inability to detect specific types, etc.

Inactive Publication Date: 2012-01-11
COFCO BIOTECHNOLOGY CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Purpose of the present invention is in order to overcome adopting prior art to detect the sugar in the citric acid fermentation clear liquid, can only detect the residual sugar total amount, and can't detect the specific kind of the sugar in the citric acid fermentation clear liquid and can't quantify accurately Defects in the content of various sugars, providing an analysis method for the citric acid fermentation serum that can detect the specific types of sugar in the citric acid fermentation serum and can accurately quantify the content of various sugars

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  • Analysis method of citric acid fermentation aqueous solution
  • Analysis method of citric acid fermentation aqueous solution
  • Analysis method of citric acid fermentation aqueous solution

Examples

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Effect test

Embodiment 1

[0030] This example is used to illustrate the analysis method of the citric acid fermentation serum provided by the present invention.

[0031] (1) Preparation of citric acid fermented liquid: the citric acid fermented liquid obtained after stirring and culturing for 20 hours according to the above-mentioned method is 4.21% by weight of citric acid fermented liquid, get 50mL of this citric acid fermented liquid, centrifuged at 4000rpm for 5min to obtain the supernatant, namely the citric acid fermentation supernatant.

[0032] (2) Chromatographic analysis: the 10mL citric acid fermentation supernatant liquid that step (1) gained is diluted 10 times with mobile phase (the mobile phase is sulfuric acid aqueous solution (concentration is 0.005mol L -1 , pH is 2.15) mixed with acetonitrile in a volume ratio of 98.5: 1.5) to obtain a mixed solution. At 60°C, and driven by the eluent at a flow rate of 0.6mL / min, 20uL of the mixed solution was passed into the high-performance liquid...

Embodiment 2

[0035] This example is used to illustrate the analysis method of the citric acid fermentation serum provided by the present invention.

[0036] (1) Preparation of citric acid fermented liquid: the citric acid fermented liquid obtained after stirring and cultivating for 30 hours according to the above-mentioned method is 5.26% by weight of citric acid fermented liquid, get 50mL of this citric acid fermented liquid, centrifuged at 4000rpm for 5min to obtain the supernatant, i.e. the citric acid fermentation supernatant

[0037] (2) Chromatographic analysis: the citric acid fermentation supernatant liquid of step (1) gained is diluted 5 times with mobile phase (the mobile phase is sulfuric acid aqueous solution (concentration is 0.005mol L -1 , pH is 2.15) mixed with acetonitrile in a volume ratio of 96.5:3.5) to obtain a mixed solution. At 60°C, and driven by the eluent at a flow rate of 0.6mL / min, 20uL of the mixed solution was passed into the high-performance liquid chromatog...

Embodiment 3

[0040] This example is used to illustrate the analysis method of the citric acid fermentation serum provided by the present invention.

[0041] (1) Preparation of citric acid fermented liquid: the citric acid fermented liquid obtained after stirring and cultivating for 40 hours according to the above-mentioned method is 10.21% by weight of citric acid fermented liquid, get 50mL of this citric acid fermented liquid, centrifuged at 4000rpm for 5min to obtain the supernatant, namely the citric acid fermentation supernatant.

[0042] (2) Chromatographic analysis: the citric acid fermentation supernatant liquid of step (1) gained is diluted 10 times with mobile phase (the mobile phase is sulfuric acid aqueous solution (concentration is 0.005mol L-1, pH is 2.15) and acetonitrile by volume ratio of 95:5) to obtain a mixed solution. At 40°C, and driven by the eluent at a flow rate of 0.5mL / min, 20uL of the mixed solution was passed into the high-performance liquid chromatograph, so t...

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Abstract

The invention discloses an analysis method of a citric acid fermentation aqueous solution, comprising the following steps of: using a chromatographic column which contains a reversed phase chromatographic separating column and an ion-exclusion chromatographic column connected in series; introducing a mixed solution of the citric acid fermentation aqueous solution and a mobile phase from an upper end of the reversed phase chromatographic separating column under the condition of chromatographic separation, respectively contacting with the reversed phase chromatographic separating column and the ion-exclusion chromatographic column, continuously washing out the reversed phase chromatographic separating column and the ion-exclusion chromatographic column by the use of an eluent, leading out the eluant A from a lower end of the ion-exclusion chromatographic column, carrying out chromatography to obtain retention time and peak area, comparing with a standard spectrogram obtained under the same condition to obtain sugar components of the citric acid fermentation aqueous solution, contents of each component in the sugar and the content of citric acid in the fermentation aqueous solution, wherein the eluant has the same composition with the mobile phase. By the adoption of the method provided by the invention to detect the citric acid aqueous solution, citric acid and the sugar can be separated from each other, and concrete components of the sugar, the content of each component in the sugar and the content of citric acid can also be detected. The analysis method provided by the invention is simple.

Description

technical field [0001] The invention relates to an analysis method of citric acid fermentation serum. Background technique [0002] Domestic citric acid production is based on corn and other starchy substances as raw materials. Under the action of α-amylase, the α-1,4 glycosidic bonds in the starch chain are hydrolyzed. The final products of amylopectin are glucose, maltose, maltotriose and α-limit dextrin; the final products of amylose are glucose, a small amount of maltose and maltotriose. Sugar detection is the most common indicator for controlling the citric acid production process. At present, the production of citric acid mostly uses Fehling's method to detect the residual sugar in the citric acid fermentation liquid. The hydrolysis reaction produces reducing monosaccharides, and the monosaccharides are titrated with Fehling's reagent, and the content of sugars is detected with methylene blue as an indicator, but this method can only detect the total amount of residu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 满云孙凤周勇陈影王慧娟
Owner COFCO BIOTECHNOLOGY CO LTD
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