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Method for treating amide pesticide waste water in situ by using horse radish peroxidase (HRP)

A technology of horseradish peroxidase and pesticide wastewater, applied in biological water/sewage treatment, chemical instruments and methods, water/sludge/sewage treatment, etc., can solve problems such as difficult to achieve results and high requirements, and achieve low cost , good catalytic performance and simple equipment

Active Publication Date: 2013-06-19
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This type of wastewater has high requirements for treatment conditions, and it is difficult to achieve the effect of conventional water treatment technology, so it needs to be diluted with water

Method used

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  • Method for treating amide pesticide waste water in situ by using horse radish peroxidase (HRP)
  • Method for treating amide pesticide waste water in situ by using horse radish peroxidase (HRP)
  • Method for treating amide pesticide waste water in situ by using horse radish peroxidase (HRP)

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Removal of metolachlor and its intermediate MEA: prepare a simulated water sample with a pH of 4.3, in which the concentration of metolachlor is 100ppm, the concentration of MEA is 470ppm, the concentration of HRP is set to 1.5UI / mL, and the HRP concentration is 1.5UI / mL. 2 o 2 The concentration is 18.1 mmol / L, the reaction temperature is 32° C., and the solution is placed in a shaker with a rotation speed of 200 rpm. As the reaction time increases, reddish-brown oily matter is generated in the system, and it is separated out from the water body ( figure 1 ). The reaction solution was taken out at different time points, and the concentration of metolachlor and MEA was determined by Hitachi L2000HPLC equipped with an ultraviolet detector, and the COD in the aqueous solution was tested by the national standard potassium dichromate method. The removal rate of metolachlor was 48%, MEA was completely removed, and COD was reduced by 38%. The new method of the invention is ...

Embodiment 2

[0028] Removal of alachlor and its intermediate DEA: Prepare a simulated water sample with a pH of 4.3, in which the concentration of alachlor is 100ppm, the concentration of DEA is 520ppm, the concentration of HRP is set to 1.5UI / mL, and the HRP concentration is 1.5UI / mL. 2 o 2 The concentration is 18.1 mmol / L, the reaction temperature is 32° C., and the solution is placed in a shaker with a rotation speed of 200 rpm. As the reaction time increases, a reddish-brown oily substance is formed in the system and precipitates out of the water body. The reaction solution was taken out at different time points, and the concentration of alachlor and DEA was determined by Hitachi L2000HPLC equipped with an ultraviolet detector, and the COD in the aqueous solution was tested by the national standard potassium dichromate method. The removal rate of alachlor was 55%, the removal rate of DEA was 78%, and the COD was reduced by 29%. The new method of the invention is applied to the alachl...

Embodiment 3

[0030] Removal of acetochlor and its intermediate MEA: Prepare a simulated water sample with a pH of 4.3, in which the concentration of acetochlor is 100ppm, the concentration of MEA is 470ppm, the concentration of HRP is set to 1.5UI / mL, and the HRP concentration is 1.5UI / mL. 2 o 2 The concentration is 18.1 mmol / L, the reaction temperature is 32° C., and the solution is placed in a shaker with a rotation speed of 200 rpm. As the reaction time increases, a reddish-brown oily substance is formed in the system and precipitates out of the water body. The reaction solution was taken out at different time points, and the concentration of acetochlor and MEA was determined by Hitachi L2000HPLC equipped with an ultraviolet detector, and the COD in the aqueous solution was tested by the national standard potassium dichromate method. The removal rate of acetochlor was 70%, MEA was completely removed, and COD was reduced by 40%. The new method of the invention is applied to the acetoch...

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Abstract

The invention relates to a method for treating amide pesticide waste water in situ by using horse radish peroxidase (HRP), and belongs to the technical field of the treatment of pesticide waste water. The method is characterized in that an aniline intermediate in the waste water is catalyzed by the HRP serving as a catalyst and H2O2 to form oily polymer, amide pesticides and other synthetic intermediates in the pesticide waste water are flocculated, the speed of enzymatic reaction is high, and the treatment efficiency is high; and the HRP and the H2O2 are wide in application range, low in cost and readily available, and an operation method is simple and low in input cost. The method has the advantages that the amide pesticide waste water is treated by the HRP and the H2O2, the concentration of raw pesticides, pesticide intermediates and other organic matters is reduced, and other pollutants and secondary pollution are avoided, so the method is a novel, high-efficiency and low-cost waste water treatment method.

Description

technical field [0001] The invention belongs to the technical field of pesticide wastewater treatment, and relates to a new method for treating amide pesticide wastewater, in particular to a high-efficiency, low-cost, pollution-free wastewater treatment method and its application. Background technique [0002] According to the toxicity and environmental behavior of the amide herbicides alachlor, acetochlor, metolachlor, pretilachlor, propachlor and butachlor, firstly these pesticides directly threaten human health; secondly, these The toxicity of the compound to fish ranges from poisonous to highly toxic, with LC50≤0.5mg / L for various fish; third, it is highly toxic to aquatic plants and phytoplankton, and is highly toxic to some algae. Therefore, alachlor, Acetochlor and butachlor technical substances are identified as characteristic pollutant control items. The synthetic intermediates of these pesticides, 2,6-diethylaniline (DEA) and 2-methyl-6-ethylbenzene (MEA)amine, be...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/00C02F101/38
Inventor 蔡喜运林玲云陈景文乔显亮李雪花
Owner DALIAN UNIV OF TECH