Noggin-derived peptide and use thereof

A BMP-4, BMP-7 technology, applied in the field of peptides and compositions containing the peptides, can solve the problems of stability and high molecular weight, poor application, high cost and the like

Active Publication Date: 2012-02-08
CAREGEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Also, complex purification processes are necessary to remove contamination sources originating from Escherichia coli (E.coli), and stability and high molecular weight issues, as well as high cost, make it less well-suited for use

Method used

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  • Noggin-derived peptide and use thereof
  • Noggin-derived peptide and use thereof
  • Noggin-derived peptide and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Synthetic example

[0088] [Synthesis Example: Synthesis of Glu-Leu-Ile-Glu-His-Linker-Arg-Pro-Ala-Asp (SEQ ID NO: 1)]

[0089] 700 mg of chlorotrityl chloride (CTL) resin (Nova Biochem, Cat No. 01-64-0021 ) was put into the reactor and stirred for 3 minutes after adding 10 mL of dichloromethane (MC). After removing the solvent, 10 mL of dimethylformamide (DMF) was added. Then, after stirring for 3 minutes, the solvent was removed again. After adding 10 mL of dichloromethane (DCM) to the reactor, 200 mmol of Fmoc-Asp(tBu)-OH (Bachem, Swiss) and 400 mmol of diisopropylethylamine (DIEA) were added and dissolved well by stirring. After stirring the reaction for 1 h, the mixture was washed and dissolved with methanol and DIEA (2:1) in DCM. After reacting for 10 minutes, the mixture was washed with excess DCM / DMF (1:1). After removing the solvent, 10 mL of DMF was then added and stirred for 3 minutes, and the solvent was removed again. After adding 10 mL of deprotection solution (20% piperidine in...

Embodiment 2

[0110] [Example 2: Preparation of nanopeptides]

[0111] 50 mg of the peptide prepared in Synthesis Example was accurately weighed, and dissolved into 500 mL of distilled water by shaking well. The resulting solution was mixed with 5 g of lecithin, 0.3 mL of sodium oleate, 50 mL of ethanol and a small amount of oil, and the volume was adjusted to 1 L with distilled water. The solution was prepared into 100 nm-sized nanobodies using a microfluorometer under high pressure. Nanobodies were prepared at a final concentration of about 50 ppm, and they were used as ingredients in cosmetics.

preparation example 1

[0112] [Preparation Example 1: Lotion]

[0113] A body lotion containing the peptide nanobody prepared in Example 2 was prepared according to a commonly used method as follows.

[0114] 【Table 2】

[0115] Element

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PUM

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Abstract

The present invention relates to a noggin-derived peptide and to a composition containing same for promoting hair growth, enhancing the condition of the skin, providing an anti-inflammatory function, or preventing or treating bone diseases. The noggin-derived peptide of the present invention performs a function identical or similar to that of natural human noggin, and has a superior stability and skin permeability as compared to the natural human noggin. The composition containing the peptide of the present invention as an active ingredient exhibits remarkably superior effects in treating, preventing, or improving growth factor-related symptoms, e.g. hair loss, skin conditions or stab wounds, and in treating, preventing, or improving symptoms of growth factor over-expression.; Therefore, the superior activity and stability of the peptide of the present invention are greatly advantageous for using the peptide of the present invention in medicine, quasi-drugs, and cosmetics.

Description

【Technical field】 [0001] The present invention relates to noggin-derived peptides and compositions containing said peptides. 【Background technique】 [0002] Hair follicles are skin organs unique to mammals. At the base of the hair follicle is a large structure called the papillae (Stenn and Paus, Physiol. Rev., 81:449 (2002)). The papillae are essential in the normal circulation of the hair follicle and growth of the hair shaft (Oliver, Embryol. Exp. Morph. 15:331 (1966); Oliver, Embryol. Exp. Morph. 16:231 (1967)). The hair shaft is a linear structure composed of epithelial cells containing keratin filaments with filaggrin closely attached thereto. [0003] Human hair follows a growth cycle with 3 distinct phases: anagen, catagen and telogen. The hair growth cycle is regulated by hormones or many growth factors. Severe stress or malnutrition can progress to catagen and telogen phases, leading to severe alopecia (Vladimir A. Botchkarev, American Journal of Pathology, 162...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08C07K7/06C07K14/47
CPCA61K38/00A61P17/00A61P17/14A61P19/08A61P29/00C07K5/1019C07K14/475C07K2319/00C07K7/06C07K7/08C07K14/47
Inventor Y·J·钟金荣德金银美宋尚洙I·洪曹囧美S·M·金S·M·韩
Owner CAREGEN
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