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Application of transition metal oxide

A technology of transition metals and oxides, which is applied in the detection and analysis of biochemical substances, can solve the problem of high detection cost and achieve the effects of high accuracy, good stability and low price

Inactive Publication Date: 2012-03-21
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The main reason why this method cannot be widely used is the high cost of detection and the need for professional technicians

Method used

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  • Application of transition metal oxide
  • Application of transition metal oxide
  • Application of transition metal oxide

Examples

Experimental program
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Embodiment 1

[0020] For the preparation of manganese dioxide nanosheets, refer to related literature reports in J.Am.Chem.Soc.2008, 130, 15938-15943. Specifically: 20 mL loaded with 0.6M TMA and 3% H 2 o 2 To the solution was added 10 ml of 0.3 M manganese chloride solution. The resulting suspension was stirred at room temperature for 12 hours, then centrifuged, washed with Milli-Q water and absolute ethanol, and freeze-dried. Meanwhile, other manganese dioxide nanoparticles, namely nanospheres.

[0021] For manganese dioxide nanoneedles and nanorods, refer to the hydrothermal method reported in J.Cry.Grow.2008, 310, 716-722 for the synthesis method. Specifically: 2 mM potassium permanganate manganese sulfate and 2 mM manganese sulfate were dissolved in Milli-Q water (80 ml). These products were then transferred to a reaction vessel, sealed, and heated at 160°C for a hydrothermal reaction time of 0.5-8 to 72 hours, then centrifuged, washed with Mili-Q water, and finally freeze-dried (s...

Embodiment 2

[0030] Take 10 μg each of the manganese oxide nanosheets (a), manganese oxide nanospheres (b), manganese oxide nanowires (c), and manganese oxide nanocomposites (d) obtained in the above examples, respectively, add 50 to 800 μM TMB, and react for 5 minutes. The absorbance of the reaction product was then measured. Draw the curve of TMB concentration and absorbance, and then calculate the kinetic constant (see image 3 ).

[0031] Simultaneously detect the dependency of manganese dioxide nanowire and HRP on pH value (1-12), temperature (5-95 ℃) catalytic activity (see Figure 4 ), studies were carried out using hydrogen peroxide concentrations (0-400 mM). The stability of MnO2 and HRP nanowires at pH and temperature was studied in a series of studies (see Figure 5 ).

[0032] The previously mentioned kinetic experiments were carried out by changing the reaction conditions, and the reaction rate mechanism was studied from 50 to 800 μM TMB in the absence of hydrogen peroxide...

Embodiment 3

[0034] Anti-sulfate-reducing bacteria antibody (0.5mg mL -1 ) into the wells of the above polystyrene multiwell plate and incubated overnight at 4°C, then added 1% BSA to each well to block non-specific sites and washed three times with PBS (pH 7.4); finally added dropwise to each well Different dilutions of SRB were incubated in 96-well plates for two hours (from 1.8×10 1 cfu mL -1 to 1.8×10 8 cfu mL -1 ), then add HRP-labeled rabbit anti-mouse immunoglobulin (Wuhan Boster Biological Engineering Co., Ltd.) or manganese dioxide-labeled anti-sulfate-reducing bacteria antibody (0.1mg mL -1 ), and incubated for 1 hour. Finally, for the determination of the surface activity of bacterial cells in which the horseradish peroxidase-labeled secondary antibody or manganese dioxide-labeled antibody binds to peroxidase, a substrate solution (50 μM TMB, pH 5.1). The manganese dioxide nanomaterial samples obtained in the above examples were added to the porous plate respectively, and 1...

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Abstract

The invention relates to detection and analysis of biochemical materials, in particular to an application of a transition metal oxide. The transition metal oxide is used as a signal label and used for detecting the content of specific identification biological molecules. According to the invention, the biological molecules, such as a protein factor, nucleic acid, microbe, virus and the like, are rapidly detected and analyzed by using the transition metal oxide as the signal label, the change of microbial populations is detected and controlled by combining the specific identification of the microbe and the antibody and the characteristics of transition metal oxide nano material similar to a catalytic enzyme, and microbes harmful to the human body and the environment can be rapidly detected. Compared with the traditional measurement of visible light density, the application has remarkable specificity to the detected microbes by using the characteristic of the transition metal oxide, and has high accuracy. By using the immunoreactions of the label of the transition metal oxide nano material, the application has the advantages of good stability, difficulty in inactivation, low price and the like.

Description

technical field [0001] The invention relates to the detection and analysis of biochemical substances, in particular to the application of a transition metal oxide. Background technique [0002] At present, many methods have been used for the detection and analysis of biochemical substances. The traditional method uses the maximum possible number method to control the change of the microbial population. This method requires a long time of pre-enrichment, and then passes relevant biochemical tests. This series of complex processes takes about 15 days. In addition, enzyme-linked immunosorbent reaction, fluorescent immunoblotting technology and improved maximum probable number method are also used in the detection of SRB. Although these techniques have great application prospects, there are some problems when applied to in situ in-line detection. For example, because of the long growth period, SRB needs several days to obtain sufficient metabolites, which makes the improved MP...

Claims

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Application Information

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IPC IPC(8): G01N33/569
Inventor 万逸张盾
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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