Banded sclerotial blight resistance identification method of wheat seedlings

Abstract

The invention relates to a banded sclerotial blight resistance identification method of wheat seedlings. The method comprises the following steps of: soaking wheat grains of wheat varieties or strains to be identified at 20 DEG C to germinate the wheat grains to the seedlings of 4-6 mm; placing the germinated seedlings into a wheat banded sclerotial blight hypha suspension solution to be soaked and inoculated; placing the inoculated wheat grains on a napkin which is wetted by distilled water; folding and covering the napkin into a cylinder and placing the cylinder into a centrifuge tube frame; placing the centrifuge tube frame into a temperature-control and humidity-control illumination growing box to be cultured; in the first three days of culturing, adopting culturing conditions that the temperature is 10 DEG C and the humidity is 90%; after three days, adopting culturing conditions that the temperature is 15 DEG C and the humidity is 70%; continuously culturing for 20 days and thentaking out the seedlings to inspect morbidity situations of the banded sclerotial blight; and calculating disease indexes and predicating the banded sclerotial blight resistance of the wheat seedlings according to the disease indexes. The method provided by the invention has the advantages that the identification is time-saving and labor-saving and is not limited by the growth season of the wheatand the identification result is reliable.

Description

technical field [0001] The invention relates to the field of wheat plant protection and breeding. Under indoor temperature and humidity control conditions, Rhizoctonia graminearum hyphae are used to infect newly germinated wheat shoots, and the sheath blight of wheat seedlings in the three-leaf period is investigated. Disease incidence and prediction of sheath blight resistance in wheat seedlings. Background technique [0002] Wheat sheath blight, also known as Wheat sharp eyespot, is an important worldwide disease caused by Rhizoctonia cerealis Vander hoeven and Rhizoctonia solani Kuhn. Soil-borne fungal diseases were reported abroad as early as 1934. In 1973, the disease was also discovered in my country. Since the 1980s, with the continuous improvement of fertilization level, the change of farming system and the large-scale promotion of susceptible varieties, sheath blight has become the main disease in the wheat region of the Yangtze River Basin in my country, and grad...

AI Technical Summary

Problems solved by technology

[0004] At present, the identification of wheat sheath blight resistance at home and abroad is mainly aimed at the resistance of wheat sheath blight at the adult plant stage after the jointing booting stage, and most of them are carried out by natural disease nursery or artificial inoculation, and the natural disease nursery adopts fields with severe disease As an identification nursery [Li Sishen, Liu Aixin, Li Qiang. Research progress on wheat sheath blight resistance. Journal of Shandong Agricultural University, 1999, 30(1): 85-90]; the methods of artificial inoculation mainly include: soil surface inoculation method, ditch Band inoculation method and toothpick inoculation method [Yan Wei, Cai Shibin, Wu Jizhong, Zhang Xianyi, Wu Xiaoyou. Comparison of different inoculation methods for wheat sheath blight. Journal of Anhui University of Science and Technology, 2007, 21(5): 8-12]. Each method has advantages and disadvantages, and the results of resistance identification are easily affected by environmental conditions, especially temperature, humidity, and planting density of wheat plants during inoculation and pathogenicity

The study also found that the resistance of wheat sheath blight at the seedling stage and the adult plant stage is not consistent [Chen Ying, Li Wei, Zhang Xiaoxiang, Zhang Boqiao, Yu Hanshou, Chen Huaigu. Population composition and analysis of wheat sheath blight at 33 degrees north latitude in China Research on pathogenicity. Acta of Wheat Crops, 2009, 29(6): 1110-1114], the method for identifying the sheath blight resistance of seedlings in the 3-leaf period has not been reported at home and abroad so far. Therefore, it is necessary to establish an effective The identification method of resistance to sheath blight of wheat seedlings is of great significance for screening new germplasm and new varieties resistant to sheath blight of wheat and reducing the yield loss caused by wheat sheath blight damage

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