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Reagent kit for rapidly detecting ammonium and nitrogen in water for aquaculture and detection method thereof

A detection kit, aquaculture technology, applied in the direction of chemical reaction of materials for analysis, chemiluminescence/bioluminescence, etc., can solve problems such as excessive ammonia nitrogen, breeding hazards, etc., achieve broad application prospects, easy to preserve, reduce The effect of testing costs

Inactive Publication Date: 2012-03-28
TIANJIN SHENGJI GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In the process of aquaculture, we often encounter the problem of excessive ammonia nitrogen in the pond. This problem is more serious in high-density intensive ponds, which has caused certain harm to the aquaculture.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A rapid detection kit for ammonia nitrogen used in aquaculture is characterized in that the kit is equipped with:

[0027] 10ml stoppered cuvette: 1;

[0028] Color comparison card: 1; (use the existing color comparison card)

[0029] Straw: 1;

[0030] 20ml dropper bottles: 2;

[0031] Reagent A is potassium sodium tartrate: 20ml;

[0032] Reagent B is Nash reagent: 20ml;

[0033] Preparation of Potassium Sodium Tartrate (100ml): Weigh 50g of Potassium Sodium Tartrate and dissolve it in 100ml of ammonia-free water, add 5ml of 250g / L NaOH, heat to boil, cool down and dilute to 100ml with deionized water, pack in 20ml / bottle ,Keep away from light;

[0034] Preparation of Nash reagent (400ml): Weigh 20g of potassium iodide (KI) and dissolve it in 25ml of ammonia-free water, add 8.2g of HgCl while stirring 2 Stir well until the precipitate is dissolved; take another 60g NaOH and dissolve it in 250ml deionized water, mix it after cooling, and dilute it to 400ml with ...

example 1

[0041] Ammonia nitrogen rapid detection kit precision test, the specific operation is:

[0042] The prepared ammonia nitrogen rapid detection kit was tested for repeatability, intermediate precision and reproducibility of the test samples respectively. Repeatability test: within the specified range, design 8 solutions with different ammonia nitrogen contents (measured by spectrophotometer), make 3 parallels for each concentration, measure 3 parallels for each concentration 3 times, and use the obtained measurement results for evaluation ;Intermediate precision test: In this laboratory, 3 different analysts use different equipment to prepare the above 8 different concentrations of solutions, each concentration is made 3 parallels, and the 3 parallels of each concentration are measured 3 times , evaluated with the obtained measurement results; and evaluated with the obtained measurement results and the measurement results obtained from the repeatability test; reproducibility tes...

example 2

[0044] Ammonia nitrogen rapid detection kit shelf life test:

[0045] Long-term test: put the three batches of prepared kits at 4°C and store them in the dark, and test the same batch of water samples at 0, 3, 6, 9, and 12 months respectively. The results show that the ammonia nitrogen content is actually measured. The values ​​are consistent with those measured by the spectrophotometer.

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PUM

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Abstract

The invention relates to a reagent kit for rapidly detecting ammonium and nitrogen in water for aquaculture, which is internally provided with a 10ml of colorimetric cup with a plug, a colorimetric card, a suction tube, two 20ml of dropping bottles, 20ml of A reagent (sodium potassium tartrate tetrahydrate) and 20ml of B reagent (Nash reagent). A reagent detection method has the following particular steps: firstly, cleaning the colorimetric cup for two to three times by water to be detected; secondly, taking 5ml of water samples, using the suction tube to add five drops of A reagent (sodium potassium tartrate tetrahydrate), then adding five drops of B reagent (Nash reagent), and stirring uniformly; and thirdly, covering the colorimetric cup and shaking the colorimetric cup uniformly, and standing for 1-3minutes; and adhering the colorimetric cup at the blank of the colorimetric card, and comparing the color of the colorimetric cup with that of the colorimetric card, thus obtaining the ammonium and nitrogen content (mg / L) of the water sample to be detected. The reagent kit has the advantages of accuracy, rapidness, convenience, low cost and the like, does not any devices, adopts drop counting method to detect, is used for the rapid site detection of a plurality of kinds of water such as aquaculture water, domestic sewage, industrial waste water and the like; and the using method is extremely simple.

Description

technical field [0001] The invention relates to a chemical component detection kit and a detection method thereof, in particular to a rapid detection kit of ammonia nitrogen used in aquaculture and a detection method thereof. Background technique [0002] In the process of aquaculture, we often encounter the problem of excessive ammonia nitrogen in the pond. This problem is more serious in high-density intensive ponds, which has caused certain harm to the aquaculture. The ammonia nitrogen in the pond mainly comes from three ways: (1) Excrement of aquatic animals, applied fertilizers, residual bait, animal and plant carcasses contain a large amount of protein, which is decomposed by microorganisms in the pond to form amino acids, and then further decomposed (2) When oxygen is insufficient, the water body undergoes denitrification reaction, and nitrite and nitrate are decomposed under the action of denitrifying bacteria to produce ammonia nitrogen; (3) Fish can pass through gi...

Claims

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Application Information

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IPC IPC(8): G01N21/76
Inventor 伦艳霞
Owner TIANJIN SHENGJI GRP CO LTD