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Breeding method of gerbera hybrida tissue culture plug seedlings

A tissue culture hole and plug seedling technology is applied in the breeding field of plant tissue culture plug seedlings to achieve the effects of improving the uniformity of seedlings and the speed of reproduction.

Inactive Publication Date: 2012-04-18
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After retrieval, there is no report on the technology of gerbera tissue culture plug seedlings at home and abroad

Method used

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  • Breeding method of gerbera hybrida tissue culture plug seedlings
  • Breeding method of gerbera hybrida tissue culture plug seedlings
  • Breeding method of gerbera hybrida tissue culture plug seedlings

Examples

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Effect test

Embodiment 1

[0038] (1) Obtaining sterile materials

[0039] Take young gerbera flower buds with a diameter of less than 1cm and no florets exposed, rinse them with running water for 20 minutes, then place the buds in a sterile bottle in an ultra-clean workbench, soak them in 70% alcohol for 30 seconds, add 0.1% mercury liter Its volume is soaked in 0.1% Tween-80 for 8 minutes, and then rinsed with sterile water for 4-6 times before use.

[0040] (2) Induction and differentiation of adventitious buds

[0041] Use tweezers to peel off all the bracts on the buds from the sterile material obtained in step (1), then cut off the florets on the surface of the buds and the base of the bracts, and the remaining receptacle, cut the receptacle into 4 pieces, and inoculate them on adventitious buds to induce differentiation On the culture medium, place the culture bottle at a temperature of 25°C, light intensity of 1500-2500lx, and light time of 14 hours / day for cultivation. The explants will expand...

Embodiment 2

[0056] (1) Obtaining sterile materials

[0057] Take young gerbera flower buds with a diameter of less than 1cm and no florets exposed, rinse them with running water for 20 minutes, then place the buds in a sterile bottle in an ultra-clean workbench, soak them in 70% alcohol for 30 seconds, add 0.1% mercury liter Its volume is soaked in 0.1% Tween-80 for 10 minutes, and then rinsed with sterile water for 6 times before use.

[0058] (2) Induction and differentiation of adventitious buds

[0059] Use tweezers to peel off all the bracts on the buds from the sterile material obtained in step (1), then cut off the florets on the surface of the buds and the base of the bracts, and the remaining receptacle, cut the receptacle into 4 pieces, and inoculate them on adventitious buds to induce differentiation On the culture medium (same as in Example 1), the culture bottle was placed at a temperature of 25°C, with a light intensity of 1500-2500lx and a light time of 14 hours / day. The e...

Embodiment 3

[0071] (1) Obtaining sterile materials

[0072] Take young gerbera flower buds with a diameter of less than 1cm and no florets exposed, rinse them with running water for 20 minutes, then place the buds in a sterile bottle in an ultra-clean workbench, soak them in 70% alcohol for 30 seconds, add 0.1% mercury liter Its volume is soaked in 0.1% Tween-80 for 10 minutes, and then rinsed with sterile water for 4 times before use.

[0073] (2) Induction and differentiation of adventitious buds

[0074] Use tweezers to peel off all the bracts on the buds from the sterile material obtained in step (1), then cut off the florets on the surface of the buds and the base of the bracts, and the remaining receptacle, cut the receptacle into 4 pieces, and inoculate them on adventitious buds to induce differentiation On the culture medium (same as in Example 1), the culture bottle was placed at a temperature of 25°C, with a light intensity of 1500-2500lx and a light time of 14 hours / day. The e...

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Abstract

The invention relates to a breeding method of gerbera hybrida tissue culture plug seedlings, which comprises the steps of getting a sterile material, inducing the differentiation of adventitious buds, proliferating the adventitious buds, rooting the adventitious buds, performing acclimatization, transplanting plugs and the like. Compared with the traditional propagation method of gerbera hybrida, the breeding method of the gerbera hybrida tissue culture plug seedlings can improve the propagation speed of the gerbera hybrida and the neatness of the seedlings and better keep the excellent characters of a female parent. In addition, the soilless seedling cultivation method adopting the transplantation of the plugs can not only reduce the consumption of soil resources and greatly improve the survival rate of the transplantation, but also avoid the harm caused by spread of soil-borne pathogens through germchits, thereby being an effective method suitable for factory production of gerbera hybrida germchits.

Description

technical field [0001] The invention relates to a breeding method of plant tissue culture plug seedlings, in particular to a breeding method of tissue culture plug seedlings of gerbera chrysanthemum. Background technique [0002] Gerbera, also known as gerbera, has huge flowers, tall and straight flower branches, rich colors, different postures, strong decoration, high yield, long vase life, and annual production under suitable conditions. It is the most important flower in the world's flower market. One of the cut flowers, accounting for the fifth place in the world's cut flower sales. As a large fresh-cut flower species, gerbera occupies an important position in my country and is being loved by more and more people. Its cultivation area is second only to roses, lilies and carnations. There is an urgent need for high-quality gerbera seedlings in production. [0003] The heterozygosity of gerbera varieties is high, and the offspring propagated by seeds are prone to variatio...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00
CPCY02P60/21
Inventor 毕晓颖郑洋魏秀娟程超雷家军
Owner SHENYANG AGRI UNIV
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