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Moisture-preserving springtail culture matrix and preparation method thereof

A technology for cultivating substrates and moisturizing collembola, which is applied in animal husbandry and other fields, can solve the problems of poison addition experiments, pure culture collembola culture management or test control cannot be effectively guaranteed, collembola culture substrate cannot be carried out, etc., to achieve The effect of stable and balanced humidity and proper proportion

Inactive Publication Date: 2012-04-18
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that the existing collembola culture substrate cannot be used for poison addition experiments, pure culture, collembola culture management or test control can not be effectively guaranteed, and to provide a moisturizing collembola culture substrate and its preparation method

Method used

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  • Moisture-preserving springtail culture matrix and preparation method thereof
  • Moisture-preserving springtail culture matrix and preparation method thereof
  • Moisture-preserving springtail culture matrix and preparation method thereof

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specific Embodiment approach 1

[0013] Specific embodiment one: In this embodiment, a kind of moisturizing collembola culture substrate is composed of 1-3g of agar powder, 2-3mL of phosphate buffer and 97-98mL of distilled water; wherein the phosphate buffer is composed of 2-20g of KH 2 PO 4 and 1-3g of KOH are added to distilled water to make up to 100mL; wherein the pH of the phosphate buffer is 6.0.

[0014] The moisturizing collembola culture medium of this embodiment has a stable and balanced humidity during the collembola culture process, and does not need to replenish water. After sterilization and ultra-clean operation, it can ensure that the collembola living medium is free of sundries and bacteria, and can purify the collembola. Sexual culture, because the base of the medium is soft and the humidity is suitable, the collembola has no escape and wall climbing phenomenon.

[0015] The proportion of agar powder in the moisture-retaining collembola culture medium of this embodiment is appropriate, and...

specific Embodiment approach 2

[0016] Specific implementation mode two: the preparation method of the moisturizing collembola culture substrate of the present embodiment is as follows: 1. Weigh 2-20g of KH 2 PO 4 and 1-3g of KOH, add distilled water, adjust the pH value of the phosphate buffer to 6.0, and then adjust the volume to 100mL to prepare a phosphate buffer; 2. Weigh 1-3g of agar powder into a beaker, add 2-3mL For the phosphate buffer solution prepared in step 1, dilute to 100mL with distilled water; 3. Seal the beaker with parafilm and put it in a high-pressure steam sterilizer to sterilize at 120°C for 20min; 4. Cool down to 50-60°C after sterilization , pour the culture medium prepared in step 3 into a petri dish in an ultra-clean workbench, and the culture medium is 0.4-0.6cm thick; 5. Put the culture dish in step 4 on the test bench and wait for the culture matrix to cool and solidify. Moisturizing springtail culture substrate.

[0017] The adjustment of the phosphate buffer solution descri...

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Abstract

The invention relates to a moisture-preserving springtail culture matrix and a preparation method thereof, and relates to a springtail culture matrix and a preparation method thereof. By the springtail culture matrix and the preparation method thereof, the problem that poison addition experiments and axenic culture cannot be performed in the conventional springtail culture matrix and the problem that springtail culture management or test control cannot be ensured effectively are solved. The moisture-preserving springtail culture matrix consists of agar powder, phosphate buffer and distilled water. The preparation method comprises the following steps of: 1, preparing the phosphate buffer; 2, mixing the agar powder and the phosphate buffer; 3, sterilizing at high temperature under high pressure; 4, pouring the mixture into a culture dish under sterile environment; and 5, standing until the culture matrix is cooled and solidified. The culture matrix has an obvious moisture-preserving effect, the interference of impurities and bacteria is avoided due to high-temperature sterilization, the phenomena that springtail does not escape from the culture dish and crawl the wall are avoided, and the subculture and toxicologic tests of the springtail can be ensured. The moisture-preserving springtail culture matrix can be used for the detection of biochemical indexes and can perform the toxicologic tests of the springtail by simulating soil contamination.

Description

technical field [0001] The invention relates to a collembola culture substrate and a preparation method thereof. Background technique [0002] Collembola, commonly known as Collembola, is one of the most abundant arthropods in soil and has important ecological functions. Folsomia candida belongs to the Isotomidae family and is the dominant species in the collembola community. It is widely distributed in soil and litter, and has been used as an indicator species for soil ecological risk assessment at home and abroad for a long time; because it is easy to Cultivation and expansion, biological characteristics and ecological characteristics are relatively clear, and widely used as toxicological model organisms for environmental pollution simulation. The cultivation of Collembola is the basis and necessary condition for its simulation experiment. At present, the commonly used collembola culture substrate is a substrate composed of a certain proportion of gypsum and activated ca...

Claims

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Application Information

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IPC IPC(8): A01K67/033
Inventor 王云彪刘晶闫修民吴东辉常亮
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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