Macrobrachium nipponense AST gene, primer group for amplification of macrobrachium nipponense AST gene and amplification method thereof

A primer set, gene technology, applied in the fields of botany equipment and methods, biochemical equipment and methods, genetic engineering, etc.

Inactive Publication Date: 2012-05-02
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few relevant reports on the research on the AST gene of crustaceans at present, so the study of AST genes in crustaceans is of great significance for the study of the growth and development mechanism of crustaceans

Method used

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  • Macrobrachium nipponense AST gene, primer group for amplification of macrobrachium nipponense AST gene and amplification method thereof
  • Macrobrachium nipponense AST gene, primer group for amplification of macrobrachium nipponense AST gene and amplification method thereof
  • Macrobrachium nipponense AST gene, primer group for amplification of macrobrachium nipponense AST gene and amplification method thereof

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Embodiment 1

[0093] Extraction of total RNA: Take the brain tissue of green shrimp one by one, quickly put it into a pre-cooled mortar filled with liquid nitrogen, take a total of about 30 mg of brain tissue from five green shrimp, dissect quickly and pay attention to adding liquid nitrogen; total RNA The extraction was carried out using the RNAiso Plus extraction reagent from Takara Company combined with the traditional phenolic extraction method, and the extraction method was referred to the instruction manual.

[0094] cDNA first-strand synthesis: according to the instructions of Shanghai Sangon Bioengineering Co., Ltd. (Sangon) cDNA synthesis kit.

[0095] Cloning of the full-length cDNA sequence of the freshwater shrimp AST gene:

[0096] According to the obtained AST gene full-length cDNA sequence (sea shrimp Panulirus interruptus, AB245091.1; Crawfish clarkii Procambarus clarkii, AB106899.1; Macrobrachium rosenbergii Macrobrachium rosenbergii , DQ417120.1) coding regions were ...

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Abstract

The invention relates to the field of gene cloning, especially to macrobrachium nipponense allatostatin (AST) gene, its amplification method and an amplification primer group. By the use of the primer group provided by the invention, an AST gene full-length cDNA sequence is cloned from macrobrachium nipponense brain tissues. The invention not only provides a theoretical basis for the research of macrobrachium nipponense ecdysis regulation, gonad maturation and oocyte maturation but also establishes a theoretical basis for the improved varieties propagation of macrobrachium nipponense.

Description

technical field [0001] The invention relates to the field of gene cloning, in particular to the green shrimp AST gene, its amplification method and amplification primer set. [0002] Background technique [0003] Freshwater shrimp, scientific name Japanese river prawn ( Macrobrachium nipponense ), belonging to the order Decapoda, the family Palaemonidae, and the genus Macrobrachium, are widely distributed all over the country and are important freshwater farmed shrimps in my country. In recent years, the degradation of germplasm resources, small size, and precocious puberty caused by successive generations of breeding have become the key issues restricting the sustainable development of freshwater shrimp aquaculture. The key to solving these production problems lies in the mastery of the regulation mechanisms such as growth and development, reproduction, and molting of freshwater shrimp. [0004] Allatostatin (AST) is a neuropeptide composed of several to dozens of amino ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/16C12N15/11C12N15/10
Inventor 傅洪拓卜宪飞乔慧吴滟龚永生
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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