Method for purifying rapamycin from broth

Abstract

The invention belongs to the biomedicine technical field, concretely relates to a method for purifying rapamycin from broth. In order to raise the purity of rapamycin, reduce the impurity content in rapamycin and increase the yield of rapamycin, the invention uses mycelium extraction, broth extraction, macroporous resin adsorption, silicagel column chromatography, cooling crystallization and recrystallization to extract and purify the rapamycin pure product from the broth, the purity is greater than 99.3%, the single impurity is less than 0.10% and the yield is increased to 53-60%. According to the invention, the rapamycin purity is raised, the impurity content is reduced, the yield is enhanced simultaneously, the operation is convenient, the technology is simple, and the method for purifying rapamycin from broth has high utility value in large industrial production.

Description

technical field

[0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for purifying rapamycin from fermentation broth. Background technique

[0002] Rapamycin (RPM, RAPA), also known as Sirolimus, is a triene macrolide compound composed of 31-membered rings, containing special α, β-diketopipexamine Molecularly masked hemicarboxyketones with the formula C 51 h 79 o 13 , the molecular weight is 991KD. It was isolated from Strepomyces hygroscopicus in 1975 by the Ayerst Research Institute in Canada.

[0003] Vezinia found that it has anti-fungal effect in 1975, and later Tanaka and others found that it is a promising immunosuppressant and has anti-tumor effect. Studies by Tanaka et al. have shown that rapamycin has a strong anti-graft rejection reaction, can synergize with cyclosporine to prolong the survival time of grafts, reverse ongoing transplant rejection, and prevent and treat experimental autohemolytic anemia rol...