Screening method of excellent AM (arbuscular mycorrhizal) fungus strain

Abstract

The invention discloses a screening method of an excellent AM (arbuscular mycorrhizal) fungus strain. The method comprises the following steps: selecting normal tobacco rhizosphere soil from the tobacco planting soil in which tobacco outbreaks in areas or grows abnormally, wherein the soil sample selected in the rhizospheric soil of each plant is 3 to 8kg; filling each selected soil sample in a pot; transplanting 1 to 2 tobacco seedlings for pot culture; drawing out the tobacco plant from the pot after 70 to 90 days, cutting the tobacco root, and burying the tobacco root into the pot; continuing transplanting 1 to 2 tobacco seedlings from the tobacco root buried pot, drawing out the tobacco plant from the pot after 70 to 90 days, cutting the tobacco root, and respectively burying each tobacco root into pots which are prepared in advance and filled with the same soil sample source; transplanting the tobacco seedling with one plant in each pot, wherein fertilizer is not applied; and performing target management to select the AM fungus strain with excellent target property. The screening method has the advantages of small workload, high selection efficiency, simple method, convenience for operation, strong synergy effect, good effect and the like, and the prepared strain is a natural hybrid strain.

Description

technical field [0001] The invention relates to a method for screening excellent AM fungal strains, belonging to the field of screening arbuscular mycorrhizal fungi. Background technique [0002] Arbuscular mycorrhizal fungi ( Arbuscular mycorrhizal fungus ) referred to as AM fungus, which is widely distributed in nature. It can form mycorrhizae with more than 80% of plants on land, and can affect many metabolic processes of plants in different ways or ways. It not only affects the growth and development of plants, nutritional status, and resistance Reversibility, yield and quality play an important role, and can improve soil structure to a certain extent. [0003] Tobacco is one of the symbiotic plants of arbuscular mycorrhizal fungi. With the development of society, people pay more and more attention to the harm of smoking to the body, and the control of the application of chemical fertilizers and pesticides in the process of tobacco leaf production is becoming more and m...

AI Technical Summary

Problems solved by technology

[0005] At present, the screening of excellent AM fungal strains is suitable for scientific research and small-scale experiments. The method is to first isolate the spores of various AM fungi in the soil, and then carry out the expansion of the strains to obtain the expanded AM fungal agent Carry out in vivo test again, carry out pot test to selected AM fungus one by one, determine the characteristic of this AM fungus by different management conditions and selection index; Can find out that the screening of existing this AM fungus has serious problems by the above-mentioned process. The workload is large, the efficiency is low, etc. Generally, the screening of a target strain takes more than 3 years under the condition of collaboration of multiple scientific researchers, and the cost is very high; at the same time, because the selected AM fungus is composed of a single The spores multiply, so the resulting AM fungi only have a single target characteristic, and there are shortcomings such as synergy, synergy and poor effect among different AM fungi