Strains of s.cerevisiae, capable of growing in media with melibiose, stachyose and raffinose

A yeast and genetic technology, applied in the field of Saccharomyces cerevisiae strains, can solve problems such as changing DNA, low growth rate, interfering with replication and transcription process growth and respiration
CN102449145AInactive Publication Date: 2012-05-09UNIV DA CORUNA
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
UNIV DA CORUNA
Publication Date
2012-05-09
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to strains of S. cerevisiae capable of secreting Alpha-galactosidase into culture medium, as well as to methods for obtaining Alpha-galactosidase using said strains and methods for producing biomass and bioethanol by means of culturing said strains in media rich in galactose. In another aspect, the invention provides methods for expressing recombinant proteins using a composition rich in galactose as a culture medium for the microorganisms producing said protein.
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Description

technical field

[0001] The present invention relates to S. cerevisiae strains capable of producing alpha-galactosidase. For its better secretion, a signal sequence was fused to the gene of α-galactosidase. Said bacterial strain is used in a method for producing α-galactosidase, biomass and ethanol in a medium rich in raffinose, melibiose and / or stachytetraose, and if the bacterial strain contains The secondary construct of the functional protein can then be used to produce the therapeutic protein. Background technique

[0002] α-Galactosidase (EC 3.2.1.22) catalyzes the hydrolysis of galactose residues, which combine with galactooligosaccharides and galactomannose polymers (mannose polymers with galactose branches) via α (1,6) Glycosidic bonding; also hydrolyzes oligosaccharides such as stachyose, raffinose and melibiose present in beans, soybeans and other legumes.

[0003] These enzymes are widely distributed in animals, plants and microorganisms. But most monogastric ...

Claims

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