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Glutathione molecular imprinting polymer, preparation method and application thereof

A technology of glutathione and molecular imprinting, which is applied to the preparation method of peptides, chemical instruments and methods, peptides, etc., can solve the problems of difficult removal of pigments, difficulties in resin regeneration by ion exchange, and difficulties in glutathione, and achieve The preparation method is simple and convenient, and the effect suitable for large-scale popularization and application

Inactive Publication Date: 2012-06-06
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The current method for separating and purifying glutathione is ion exchange, but ion exchange is difficult during resin regeneration and requires a large amount of acid and alkali; there is also an isoelectric point precipitation method, but the isoelectric point precipitation method is used to separate and purify glutathione Glutathione requires a certain amount of glutathione, otherwise glutathione is difficult to precipitate; and in the process of separation and purification, it is difficult to remove the pigment by extraction with various organic solvents, and it runs through the entire purification process. middle

Method used

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  • Glutathione molecular imprinting polymer, preparation method and application thereof
  • Glutathione molecular imprinting polymer, preparation method and application thereof
  • Glutathione molecular imprinting polymer, preparation method and application thereof

Examples

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Embodiment 1

[0025] Example 1 Synthesis example of glutathione molecularly imprinted polymer (functional monomer is methacrylic acid)

[0026] Dissolve 1.0mmol of the template molecule glutathione (GSH) in 15ml of the solvent dimethyl sulfoxide, ultrasonically mix, and dissolve overnight, and then divide the solution into 3ml each (containing 0.2mmol of the template molecule) According to the molar ratio of template molecule: functional monomer = 1: 2; 1: 4; 1: 5; 1: 8; Methacrylic acid MAA; then add a corresponding amount of crosslinking agent ethylene glycol dimethacrylate EGDMA according to the molar ratio of functional monomer: crosslinking agent=1:5 (2.0; 4.0; 5.0; 8.0; 10.0mmol), and the initiator azobisisobutyronitrile AIBN. Ultrasonic degassing for 10 minutes, after ultrasonic mixing, nitrogen was introduced for 10 minutes, and the reaction system was sealed under nitrogen. React at 60°C for 24 hours; after the polymerization reaction, crush, grind, and sieve the synthesized po...

Embodiment 2

[0038] Example 2: Synthesis example of glutathione molecularly imprinted polymer (functional monomer is acrylamide)

[0039] Dissolve 1.0mmol of the template molecule glutathione (GSH) in 15ml of the solvent dimethyl sulfoxide, ultrasonically mix, and dissolve overnight, and then divide the solution into 3ml each (containing 0.2mmol of the template molecule) According to the molar ratio of template molecule: functional monomer = 1: 2; 1: 4; 1: 5; 1: 8; Acrylamide AM; then add a corresponding amount of crosslinking agent ethylene glycol dimethacrylate EGDMA according to the molar ratio of functional monomer: crosslinking agent=1:5 (2.0; 4.0; 5.0; 8.0; 10.0mmol), and Initiator azobisisobutyronitrile AIBN. Ultrasonic degassing for 10 minutes, after ultrasonic mixing, nitrogen was introduced for 10 minutes, and the reaction system was sealed under nitrogen. React at 60°C for 24 hours; after the polymerization reaction, crush, grind, and sieve the synthesized polymer, and then re...

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Abstract

The invention relates to a preparation method of a glutathione molecular imprinting polymer. The glutathione molecular imprinting polymer is prepared by using glutathione as a template molecule, carrying out polymerization on functional monomers in a protective gas with existence of cross-linking agent and initiator and removing glutathione in the polymer. Preferably, a specific preparation process comprises steps of: dissolving glutathione in a solvent; adding functional monomers to form a template molecule-functional monomer copolymer; adding the cross-linking agent and the initiator; carrying out polymerization protective gas to obtain the polymer; and washing out glutathione in the polymer. The invention also provides the glutathione molecular imprinting polymer prepared by the method and a method for separating glutathione. The glutathione molecular imprinting polymer of the invention can specifically and selectively separate glutathione, enrich glutathione and provide basis for subsequent application. The preparation method is simple and convenient and suitable for large-scale popularization and application.

Description

technical field [0001] The present invention relates to the technical field of molecularly imprinted polymers, in particular to the technical field of glutathione molecularly imprinted polymers, in particular to a glutathione molecularly imprinted polymer and its preparation and application method, especially suitable for the separation and purification of glutathione peptide. Background technique [0002] Glutathione (GSH) is a natural active tripeptide (L-γ-glutamyl-L-cysteinyl-glycine) with important physiological functions. A variety of metabolic processes can protect cells in living organisms from oxidation and toxic substances; it has a wide range of uses in clinical medicine, food industry, cosmetics industry and other fields. Recent studies have found that GSH also has the effect of inhibiting AIDS. [0003] The current method for separating and purifying glutathione is ion exchange, but ion exchange is difficult during resin regeneration and requires a large amoun...

Claims

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Application Information

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IPC IPC(8): C08F222/14C08F220/06C08F220/56C08F2/44C08J9/26C07K1/14
Inventor 辛瑜杨海麟夏小乐张玲仝艳军张玉然王武
Owner JIANGNAN UNIV