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Mesenchymal stem cell stock solution

A technology for mesenchymal stem cells and storage solution, which is applied in the field of mesenchymal stem cell storage solution to achieve the effect of convenient long-distance supply and clinical application, convenient transportation and temporary storage

Active Publication Date: 2012-06-13
上海安库生医生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problem of transportation and temporary storage of mesenchymal stem cell preparations, the present invention provides a formula of mesenchymal stem cell storage solution, which can store mesenchymal stem cells at -20-4°C for 14-20 days The activity rate remains above 85%, and the components of the storage solution are all from injectable reagents for medicine that are safe for the human body

Method used

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  • Mesenchymal stem cell stock solution

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] I. Composition and concentration of mesenchymal stem cell storage solution

[0058]

[0059]

[0060] II. Implementation steps

[0061] (1) Take 2.0×10^8 human mesenchymal stem cells (source: extracted from the placenta donated by obstetrical patients of Zhongshan Hospital Affiliated to Fudan University in Shanghai), centrifuge to discard the supernatant, wash twice with normal saline, and add 200ml of mesenchymal stem cells The stem cell storage solution was resuspended, counted by an automatic cell counter, and the cell concentration was adjusted to 1.0×10^6 cells / ml to make a mesenchymal stem cell preparation for injection, which was distributed into 20 frozen blood bags, and each bag of live cells The quantity is 1.0×10^7 pieces.

[0062] (2) Store 10 bags at 4°C and store 10 bags at -20°C.

[0063] (3) After 3 days and 15 days of storage, 5 bags of mesenchymal stem cell preparations were taken out from 4°C and -20°C respectively, and rethawed at 35°C.

[0...

Embodiment 2

[0069] I. Composition and concentration of mesenchymal stem cell storage solution

[0070]

[0071]

[0072] II. Implementation steps

[0073] (1) Take 2.0×10^8 human mesenchymal stem cells (source: extracted from the placenta donated by obstetrical patients of Zhongshan Hospital Affiliated to Fudan University in Shanghai), centrifuge to discard the supernatant, wash twice with normal saline, and add 200ml of mesenchymal stem cells The stem cell storage solution was resuspended, counted by an automatic cell counter, and the cell concentration was adjusted to 1.0×10^6 cells / ml to make a mesenchymal stem cell preparation for injection, which was distributed into 20 frozen blood bags, and each bag of live cells The quantity is 1.0×10^7 pieces.

[0074] (2) Store 10 bags at 4°C and store 10 bags at -20°C.

[0075] (3) After 3 days and 15 days of storage, 5 bags of mesenchymal stem cell preparations were taken out from 4°C and -20°C respectively, and rethawed at 35°C.

[0...

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Abstract

Relating to the field of cell biology, the invention discloses a mesenchymal stem cell stock solution, which comprises glucose, sodium chloride, albumin, propylene glycol, cholesterol, and sodium bicarbonate, etc., and also can contain heparin. The mesenchymal stem cell stock solution provided in the invention can realize storing a mesenchymal stem cell at a temperature of -20-4DEG C for 14-20 days and maintaining a motility rate of over 85%, thus being convenient for cell transport and temporary storage. Also, the stock solution of the invention has components all coming from medicinal injectable reagents that are safe for human bodies, and can be injected directly after unfreezing, thus providing great convenience for long distance supply and clinical application of mesenchymal stem cell preparations.

Description

technical field [0001] The invention relates to the field of cell biology, in particular to a mesenchymal stem cell storage solution. Background technique [0002] Mesenchymal stem cells (MSCs, Mesenchymal Stem Cells) are derived from mesoderm and ectoderm in the early stages of development. focus on. Mesenchymal stem cells were first found in the bone marrow, and later found in many tissues in the process of human development and development, such as bone marrow, fat, synovium, bone, muscle, lung, liver, pancreas and other tissues, as well as amniotic fluid and umbilical cord blood , placenta, etc. [0003] Under specific induction conditions in vivo or in vitro, mesenchymal stem cells can differentiate into fat, bone, cartilage, muscle, tendon, ligament, nerve, liver, cardiac muscle, endothelial and other tissue cells. It has multi-directional differentiation potential and can be used for various purposes such as life science research, drug screening test, tissue and or...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 陈英波郑佳威杨立敏金宜强
Owner 上海安库生医生物科技有限公司
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