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Method for producing active Rana dybowskii collagen oligopeptide by using compound enzyme

A compound enzyme and original protein technology, which is applied in the field of collagen oligopeptide preparation, can solve the problems of dispersion, large molecular weight, and low extraction rate of single enzyme method, and achieve the effects of reducing pollution, simplifying operation procedures, and reducing costs

Active Publication Date: 2012-06-13
吉林省轻工业设计研究院
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

There are also many domestic studies on the enzymatic hydrolysis of marine collagen. The molecular weight of short peptides obtained by papain enzymatic hydrolysis of fish skin is 6000-29000D. The extraction rate of the single enzyme method used in this method is low, and the molecular weight is large and dispersed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Accurately weigh 1 kg of dried Rana skin, add water at room temperature until completely soaked Rana skin, soak in lime and soften for 8 hours, stir several times until the skin is completely softened and the color of the skin is completely dissolved, stir evenly, measure and adjust the pH The value is 6.8 to 7.2.

[0017] When the material is transferred to the reaction kettle and heated to 60-65°C, a compound enzyme whose mass is 1% of the weight of the dried wood frog is added. 50 rpm, enzymatic hydrolysis for 3 hours. After 20 minutes at 85°C, the enzyme was inactivated, decolorized using activated carbon with a weight of 3% to 5% of the weight of the dry Northeast wood frog, refined by ultrafiltration membrane, concentrated, and vacuum freeze-dried to obtain 350g of wood frog collagen oligopeptides. The oligopeptide product of Rana collagen is slightly yellow, the rehydration time is short, the water solubility is good, the oligopeptide content is 97%, and the hea...

Embodiment 2

[0019] The method is the same as in Example 1, the extraction pH value during enzymolysis is 7.0 to 7.5, and the other conditions are the same as in Example 1 to obtain 320 g of the product, the extraction rate is 32%, the product color is light yellow, the rehydration time is shorter, and the water solubility is better, low Polypeptide content 95%, heavy metal content 1.9ppm.

Embodiment 3

[0021] The method is the same as in Example 1, the amount of the compound enzyme added is 1.5% of the weight of the dry Rana sinensis, other conditions are the same as in Example 1, and 330 g of the product is obtained, the extraction rate is 33%, the color of the product is light yellow, the rehydration time is short, and the water solubility is good , oligopeptide content 96%, heavy metal content 2.1ppm.

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Abstract

The invention relates to a method for producing active Rana dybowskii collagen oligopeptide by using compound enzyme, which belongs to the collagen preparation field, the method comprises the following steps: 1) liming, softening, staying overnight and washing the skin of the Rana dybowskii to neutrality; 2) adding the compound enzyme, carrying out enzymatic hydrolysis for 2-3 hours, heating to the temperature of 85 DEG C, inactivating for 20 minutes; 3) decolouring by active carbon, refining by an ultrafilter membrane, concentrating, vacuum freeze drying to obtain the micromolecule active Rana dybowskii collagen oligopeptide. The method of the invention can greatly reduce the cost, the enzymatic hydrolysis rate is more than 90%, the extraction yield is more than 30% which is increased byabout 5%, the extraction purity after enzymatic hydrolysis can reach 90-97%, the pollution on environment can be greatly minimized, the control operation is simplified, the method has the advantages of high efficiency and environmental protection.

Description

technical field [0001] The invention relates to the field of preparation of collagen oligopeptides, in particular to a method for producing active collagen oligopeptides by enzymatically hydrolyzing the skin of the Northeast forest frog with a compound protease under optimal conditions. Background technique [0002] Collagen oligopeptide is a molecular polymer after collagen biodegradation, which is composed of two or more amino acids linked by peptide bonds. It has been scientifically proven that the aging of human skin is the process of collagen loss. Its physiological effects on the human body are mainly manifested in: immune regulation, anti-oxidation, anti-fatigue, broad-spectrum antibacterial, anti-tumor, etc., and can be widely used in medicine, health food, cosmetics and other fields. At present, my country's demand for collagen is about 400 tons per year. [0003] The main sources of collagen oligopeptides can be divided into artificial synthesis and collagen enzy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 孙智谋王海波周旭戚译天
Owner 吉林省轻工业设计研究院
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