Building method for genetic linkage map based on four-way cross population
A genetic linkage map and construction method technology, applied in special data processing applications, instruments, electronic digital data processing, etc., can solve problems that cannot be used to analyze multi-parental mating populations, and are difficult to achieve
Inactive Publication Date: 2013-09-04
ZHEJIANG UNIV
View PDF1 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Among them, Mapmaker can be applied to various types of experimental populations for genetic mapping. It is one of the most widely used mapping software at present, but when it comes to linkage tests with a large number of markers (more than 1000 markers), it is often difficult to achieve, and this The program cannot be used to analyze polyparental mating populations
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0069] The present invention will be further described below in conjunction with accompanying drawing.
[0070] Step (1). Collection of molecular marker information
[0071] 1-1. Prepare spotting samples in the electrophoresis experiment of molecular markers, the spotting samples include parent material and four-parent mating population material. Such as figure 1 As shown, the parent materials are four parents P1, P2, P3, P4 with obvious genetic differences, and the parents P1 and P2 are a group of crosses to generate the F1 parent PA, and the parents P3 and P4 are a group of crosses to generate the F1 parent PB; The parental mating population is produced by crossing F1 parents PA and PB; the number of molecular markers is m, and the number of four-parental mating populations is n, where n>100;
[0072] 1-2. If figure 2 As shown, observe the tapes of m molecular markers in the electrophoresis experiment of molecular markers; for each molecular marker, a, b, c, and d are us...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention discloses a building method for a genetic linkage map based on four-way cross population, and aims to overcome the defect that the genetic mapping and breeding practice cannot be combined tightly in the traditional method due to narrow genetic basis. The method provided by the invention takes a four-recurrent parent mating population as the population for building the genetic linkage map, and comprises the following steps: collecting molecular marker information, preparing sample application samples in an electrophoresis experiment, observing the adhesive tape of m molecular markers in the electrophoresis experiment, estimating intervals between molecular markers, estimating the recombination ratio r of two molecular markers by adopting a maximum likelihood method, coordinating the recombination ratio r into a distance matrix of the molecular markers, building a linkage group, and arranging the molecular markers in the linkage group in sequence. The chromosome of the four-way cross population adopted in the invention has 2 to 4 equipotential locuses which can inherit difference better and increase polymorphism. Therefore, the building method improves the covering rate of genetic maps, reduces error II probability in genetic statistics caused by adopting the conventional methods.
Description
technical field [0001] The invention belongs to the technical fields of biology, mathematics and computing science, and specifically relates to a method for constructing a genetic linkage map based on four-cross populations. Background technique [0002] The construction of genetic linkage maps has important theoretical and practical value, and it has played an important role in gene mapping, map-based cloning, auxiliary marker selection, research on genome organization, and research on the origin and evolution of plants through comparative mapping. [0003] To construct a genetic linkage map, the first step is to select a suitable genetic mapping population. So far, most plant species have used the derivative populations of single self-bred populations to construct genetic linkage maps. These populations have only two alleles on chromosomes and have few genetic differences. For some species with poor genetic polymorphism, such as tobacco, cotton, etc., the effect of using...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68G06F19/10
Inventor 徐海明余世洲肖炳光马亚楠周佳萍
Owner ZHEJIANG UNIV