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Method for quickly breeding seedlings of Guangxi anoectochilus roxburghii capsules by utilizing Guangxi anoectochilus roxburghii capsules

A technology of lotus capsule and golden thread is applied in the field of rapid breeding of seedlings, which can solve problems such as unseen seedlings, and achieve the effects of low production cost, maintaining excellent characters and improving safety.

Inactive Publication Date: 2012-06-20
GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the existing researches focus on clematis in Fujian, Guangdong and Taiwan, and mainly use the stem segment as the explant material for propagation. There is no report on the rapid propagation of seedlings using clematis capsules in Guangxi

Method used

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  • Method for quickly breeding seedlings of Guangxi anoectochilus roxburghii capsules by utilizing Guangxi anoectochilus roxburghii capsules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The present invention utilizes the method for quickly breeding its seedlings of Aureus roxburghii capsules, comprising the following steps:

[0020] (1) Selection of capsule maturity: Introduce the clematis plants collected in the field into the artificial resource garden, and select excellent lines at the flowering stage for artificial pollination and fruit cultivation. The color gradually changes from milky white pulp to golden yellow filament, among which uncracked capsules that have grown for 120-150 days after artificial pollination are the best materials;

[0021] (2) Germination culture of seeds: After the mature capsules are sterilized, take out the internal seed embryos and inoculate them on the germination medium 1 / 2MS+AC1.0g / L+sucrose 30g / L+agar 6.0g / L. 24~28℃, light intensity 20 μmol m -2 ·s -1 1. Under the condition of light time of 12 h / d, cultured for 25-35 days, the seeds germinated to form white protocorms, and the germination rate was 80.3%;

[0022...

Embodiment 2

[0027] The present invention utilizes the method for quickly breeding its seedlings of Aureus roxburghii capsules, comprising the following steps:

[0028] (1) Selection of capsule maturity: Introduce the Guangxi clematis plants collected in the field into the artificial resource garden, select excellent strains for artificial pollination at the flowering stage, pick capsules that grow 90-120 days after artificial pollination, and use them indoors Wash off the dust on the surface with tap water, and gently cut off the dirt on the surface with a blade, then move it to the ultra-clean workbench for aseptic operation, first soak it in 75% alcohol for 30 seconds, then transfer it to 0.1% HgCl 2 Disinfect in the solution for 8 minutes, shake continuously during the period, rinse with sterile water 5 times, dry the surface moisture of the material with sterile filter paper, take out the internal seed embryos, inoculate them on the germination medium, and inoculate them at a temperat...

Embodiment 3

[0034] The present invention utilizes the method for quickly breeding its seedlings of Aureus roxburghii capsules, comprising the following steps:

[0035](1) Selection of capsule maturity: Introduce the Guangxi clematis plants collected in the field into the artificial resource garden, select excellent strains for artificial pollination at the flowering stage, pick capsules that grow 120-150 days after artificial pollination, and use them indoors Wash off the dust on the surface with tap water, and gently cut off the dirt on the surface with a blade, then move it to the ultra-clean workbench for aseptic operation, first soak it in 75% alcohol for 30 seconds, then transfer to 0.1% HgCl 2 Disinfect in the solution for 8 minutes, shake continuously during the period, rinse with sterile water 5 times, dry the surface moisture of the material with sterile filter paper, take out the internal seed embryos, inoculate them on the germination medium, and inoculate them at a temperature...

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Abstract

The invention discloses a method for breeding seedlings of Guangxi anoectochilus roxburghii capsules by utilizing the Guangxi anoectochilus roxburghii capsules quickly. According to the method, wild anoectochilus roxburghii pollinated artificially to obtain the capsules serving as explants, and the seedlings are bred quickly in paths of seeds, protocorms, complete plants and transplantation. In the breeding process, the seeds and the protocorms are cultivated without hormones, and the protocorms are differentiated into the seedlings to form the complete small plants directly without subculture. The Guangxi anoectochilus roxburghii capsules are subjected to tissue culture for 8 to 10 months, so that more than 0.1 million plants of high-quality tissue culture seedlings can be cultivated quickly, and the survival rate of seedling-hardening transplantation is over 95 percent. The method is easy and convenient to operate, low in production cost and convenient for large-scale industrial production and application. The anoectochilus roxburghii is bred by the method, so that the maintaining of the excellent properties of the seedlings is facilitated, the use safety of the seedlings is enhanced, and the method has a positive effect on the regeneration and continuous utilization of endangered shortage medicinal plant resources.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for quickly breeding seedlings of A. Background technique [0002] Clematis [ Anoectochilus roxburghii (Wall.) Lindl.] is a perennial herbaceous plant of the genus Orchidaceae (Clematis genus). There are more than 40 species of plants in this genus, which are distributed from tropical Asia to Oceania. There are 20 species and 2 varieties in my country. Clematis clematis is a precious Chinese herbal medicine, enjoying the reputation of "King of Medicine", "Golden Grass", "Miraculous Medicine", "Black Ginseng", etc. . The germination rate of clematis seeds is extremely low under natural conditions, and resource regeneration is slow. Coupled with long-term excavation, germplasm resources are scarce and are on the verge of extinction, making it difficult to meet market demand. Guangxi clematis is one of the three main wild populations distributed in my country. I...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 付传明黄宁珍何金祥赵志国石云平唐凤鸾
Owner GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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