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Method for cultivating high-selenium-tolerability strain of edible fungi

An edible fungus and tolerance technology, which is applied in the field of preparation of high selenium materials, can solve the problems that have not yet been developed and cannot obtain high selenium tolerance bacteria, and achieves the effect of improving the ability to enrich selenium

Inactive Publication Date: 2012-06-27
SUZHOU SETEK
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current patents mainly focus on the enrichment of selenium-enriched nutrition of edible fungi and the development of agricultural products for direct consumption, such as: the preparation method of selenium-enriched pumice mushroom powder (Chinese patent application 200610135227.2), the industrialized cultivation of selenium-enriched Agaricus bisporus method (Chinese patent application 200910067431.9), has not carried out any form of using edible fungi as a high-selenium carrier, as an additive for food and health products
At present, there are two patents that have carried out some work on the selenium-enriched domestication of strains, such as: the domestication and cultivation method of selenium-enriched shiitake mushroom strains (Chinese patent application 200810010300.2), the domestication, cultivation and enrichment method of selenium-enriched Phellinus strains (Chinese patent application 200710011014.3), but it is only through simple two-stage domestication, and its purpose is not to obtain the strain with the maximum selenium-tolerant concentration, and the simple two-stage domestication method in the above two patents cannot obtain high selenium tolerance Sexual strain

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1 Obtaining the steps of Ganoderma lucidum high selenium tolerance strain

[0063] (1) Preparation of ganoderma lucidum strain with high selenium tolerance:

[0064] 1) Purchase Ganoderma lucidum strains (No. 5.653) from the Culture Collection Center of the Chinese Academy of Sciences.

[0065] 2) Insert the ganoderma lucidum into the sterilized PDA comprehensive culture medium for in vitro culture.

[0066] 3) sodium selenite (Na 2 SeO 3 ) is mixed with PDA comprehensive medium according to a certain ratio to make Na 2 SeO 3 Content is the selenium-containing culture medium of series concentration gradient of 2, 20, 50, 100, 200, 400, 600, 800, 1000 mg / L, and then sterilized for subsequent use.

[0067] 4) Inoculate with bacterial suspension, insert the test-tube strain cultivated in step 2 into the 2 mg / L selenium-containing medium prepared in step 3, and carry out primary selenium-tolerant culture of Ganoderma lucidum strain.

[0068] 5) After the first...

Embodiment 2

[0085] Embodiment 2: The steps of obtaining the high-selenium tolerance bacterial strain of Versicolor versicolor

[0086] (1) Preparation of high selenium tolerance versicolor versicolor strain:

[0087] 1) Purchase Versicolor versicolor strains (No. 5.48) from the Culture Collection Center of the Chinese Academy of Sciences.

[0088] 2) Insert the Yunzhi bacteria into the sterilized PDA comprehensive culture medium for in vitro culture.

[0089] 3) sodium selenate (Na 2 SeO 4 ) is mixed with PDA comprehensive medium according to a certain ratio to make Na 2 SeO 4 Content is the selenium-containing culture medium of series concentration gradient of 2, 20, 50, 100, 200, 400, 600, 800, 1000 mg / L, and then sterilized for subsequent use.

[0090] 4) Inoculate with bacterial suspension, insert the test-tube strain cultivated in step 2 into the 2mg / L selenium-containing medium prepared in step 3, and carry out primary selenium-resistant culture of Yunzhi strain.

[0091] 5) A...

Embodiment 3

[0110] Embodiment 3: the step of obtaining Pleurotus eryngii high selenium tolerance bacterial classification

[0111] (1) Preparation of high selenium tolerance Pleurotus eryngii strain:

[0112] 1) Purchase Pleurotus eryngii strains (No. 5.775) from the Culture Collection Center of the Chinese Academy of Sciences.

[0113] 2) Insert Pleurotus eryngii strains into the sterilized PDA comprehensive medium for in vitro strain culture.

[0114] 3) sodium selenite (Na 2 SeO 3 ) is mixed with PDA comprehensive medium according to a certain ratio to make Na 2 SeO 3 Content is the selenium-containing culture medium of series concentration gradient of 2, 20, 50, 100, 150, 200, 300, 400 mg / L, and then sterilized for standby.

[0115] 4) Adopting the method of bacterial suspension inoculation, inserting the test tube strain cultivated in step 2 into the 2 mg / L selenium-containing medium prepared in step 3, and carrying out primary selenium-tolerant cultivation of Pleurotus eryngii ...

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PUM

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Abstract

The invention discloses a method for cultivating a high-selenium-tolerability strain of edible fungi. The method is characterized in that the method comprises the the steps of: (1) selecting a culture medium suitable for growth of an edible fungi strain according to the edible fungi strain, inoculating the edible fungi strain into the culture medium to cultivate a tube strain so as to obtain initial bacterium suspension, (2) mixing the culture medium with a selenium source to prepare into selenium-tolerant medium with gradient selenium concentration, inoculating the edible fungi strain on the sterilized selenium-tolerant medium by a bacterium suspension inoculating mode, and gradually carrying out selenium-tolerant cultivation on selenium concentration by a common edible fungi strain cultivation method according to an sequence from low selenium concentration to high selenium concentration, and (3) carrying out continuous transferring cultivation by the bacterium suspension inoculating mode according to the optimal selenium-tolerant concentration of the edible fungi obtained from the step (2), and determining the selenium-tolerant character stability of the strain so as to obtain the high-selenium-tolerability strain of the edible fungi. The tolerability of selenium content in the culture medium is greatly improved a deep cultivation state of liquid after high-selenium tolerability cultivation of the edible fungi strain.

Description

technical field [0001] The invention relates to a preparation method of a high-selenium material, in particular to a method for cultivating edible fungus high-selenium tolerance strains. Background technique [0002] Selenium is an essential trace element in humans and animals. It is the active center that constitutes as many as 23 functional proteins such as glutathione peroxidase (GSH-Px) and selenoprotein P. It has the following functions: (1) remove Peroxides and free radicals can protect cell structure and function from interference and damage, and delay aging; (2) accelerate the metabolism of carcinogens, promote DNA damage repair, and reduce the incidence of cancer; (3) antagonize toxic substances in the body and reduce their (4) Enhance the body's cellular immune function and improve the body's immunity. A severe deficiency of selenium will directly lead to Keshan disease and Kaschin-Beck disease. Among them, Keshan disease was first discovered in Keshan County, Hei...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C05G1/00
Inventor 袁林喜胡海涛
Owner SUZHOU SETEK