Air-space low-interference phase microscope based on liquid crystal tunable filter

Abstract

The invention discloses an air-space low-interference phase microscope based on a liquid crystal tunable filter. The air-space low-interference phase microscope comprises an object stage, a first object lens, a first reflecting mirror, a ductoscopy and a CCD (Charge Coupled Device) image sensor and is characterized by further comprising a white light source, a spectroscope and a liquid crystal tunable filter, wherein the spectroscope is used for receiving white light signals which are focused into an observed sample through the first object lens, reflected scattered signals of the observed sample are projected into the ductoscopy through the first object lens and the first reflecting mirror, the liquid crystal tunable filter is arranged between the ductoscopy and the CCD image sensor, andthe liquid crystal tunable filter is used for projecting microscope images of different wavelengths onto the CCD image sensor. The air-space low-interference phase microscope has the obvious effects that: a light path structure is simple, the control is convenient, a system has high air-space refractive index and fluctuation sensitivity and can be used for processing a clinical sample slide directly so as to realize high-precision imaging for a cell nano-structure, and the full-field rapid measurement is realized by utilizing electro-optic liquid crystal tunning.

Description

Technical field [0001] The invention relates to a phase microscope, in particular to a spatial low-coherence phase microscope based on a liquid crystal tunable filter, belonging to optical imaging technology. Background technique [0002] Since the traditional optical microscope imaging of the sample is based on the absorption or fluorescence effect of the sample, the resolution is low ( <500nm), the change in the nanostructure of the sample cannot be detected. Optical-based phase contrast microscopes and differential interference contrast microscopes can detect tiny changes in the nanostructure of cells, but the lack of quantitative information limits their scope of application. In recent years, quantitative phase microscopes use interference effects to obtain system parameters related to cell nanostructure characteristics such as phase and amplitude, and provide quantitative information. Although this type of microscope can detect cell nanostructures, its speed, sensitivity,...

AI Technical Summary

Problems solved by technology

[0002] Since the imaging of samples by traditional optical microscopes is based on the absorption or fluorescence effects of samples, the resolution is low (<500nm), and changes in the nanostructure of samples cannot be detected.

Optical-based phase-contrast microscopy and differential interference contrast microscopy are capable of probing subtle cellular nanostructural changes, but lack of quantitative information limits their applications

In recent years, quantitative phase microscopy can obtain phase, amplitude and other system parameters related to the characteristics of cell nanostructures by using the interference effect, and provides quantitative information. Although this type of microscope can detect cell nanostructures, its speed, sensitivity and accuracy, as well as for The special requirements of the sample limit its promotion to clinical application

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