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Air-space low-interference phase microscope based on liquid crystal tunable filter

A technology for tuning filters and phase microscopy, applied in the field of spatial low-coherence phase microscopy, can solve the problems of limited application range, inability to detect sample nanostructure changes, lack of quantitative information, etc., to achieve high spatial domain refractive index fluctuation sensitivity, high Accurate imaging and simple optical path structure

Inactive Publication Date: 2012-06-27
CHONGQING UNIV
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Since the imaging of samples by traditional optical microscopes is based on the absorption or fluorescence effects of samples, the resolution is low (<500nm), and changes in the nanostructure of samples cannot be detected.
Optical-based phase-contrast microscopy and differential interference contrast microscopy are capable of probing subtle cellular nanostructural changes, but lack of quantitative information limits their applications
In recent years, quantitative phase microscopy can obtain phase, amplitude and other system parameters related to the characteristics of cell nanostructures by using the interference effect, and provides quantitative information. Although this type of microscope can detect cell nanostructures, its speed, sensitivity and accuracy, as well as for The special requirements of the sample limit its promotion to clinical application

Method used

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  • Air-space low-interference phase microscope based on liquid crystal tunable filter
  • Air-space low-interference phase microscope based on liquid crystal tunable filter

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Embodiment Construction

[0015] The present invention will be further described in detail below in conjunction with the drawings and specific embodiments.

[0016] Such as figure 1 As shown, a spatial low-coherence phase microscope based on a liquid crystal tunable filter includes a white light source 1, a beam splitter 8, a first objective lens 9, a stage 10, a first mirror 7, a tube lens 15, and a liquid crystal tunable The filter 16 and the CCD image sensor 17, in order to adjust and collimate the light of the white light source, a collimating light path is also provided between the white light source 1 and the beam splitter 8, and the collimated light is routed through the condenser lens 2, the first lens 3. The first diaphragm 4, the second lens 5 and the second diaphragm 6 are arranged in sequence.

[0017] The white light source 1 uses a xenon lamp light source, which emits white light with high intensity and low coherence. After the white light emitted by the white light source 1 passes through t...

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Abstract

The invention discloses an air-space low-interference phase microscope based on a liquid crystal tunable filter. The air-space low-interference phase microscope comprises an object stage, a first object lens, a first reflecting mirror, a ductoscopy and a CCD (Charge Coupled Device) image sensor and is characterized by further comprising a white light source, a spectroscope and a liquid crystal tunable filter, wherein the spectroscope is used for receiving white light signals which are focused into an observed sample through the first object lens, reflected scattered signals of the observed sample are projected into the ductoscopy through the first object lens and the first reflecting mirror, the liquid crystal tunable filter is arranged between the ductoscopy and the CCD image sensor, andthe liquid crystal tunable filter is used for projecting microscope images of different wavelengths onto the CCD image sensor. The air-space low-interference phase microscope has the obvious effects that: a light path structure is simple, the control is convenient, a system has high air-space refractive index and fluctuation sensitivity and can be used for processing a clinical sample slide directly so as to realize high-precision imaging for a cell nano-structure, and the full-field rapid measurement is realized by utilizing electro-optic liquid crystal tunning.

Description

Technical field [0001] The invention relates to a phase microscope, in particular to a spatial low-coherence phase microscope based on a liquid crystal tunable filter, belonging to optical imaging technology. Background technique [0002] Since the traditional optical microscope imaging of the sample is based on the absorption or fluorescence effect of the sample, the resolution is low ( <500nm), the change in the nanostructure of the sample cannot be detected. Optical-based phase contrast microscopes and differential interference contrast microscopes can detect tiny changes in the nanostructure of cells, but the lack of quantitative information limits their scope of application. In recent years, quantitative phase microscopes use interference effects to obtain system parameters related to cell nanostructure characteristics such as phase and amplitude, and provide quantitative information. Although this type of microscope can detect cell nanostructures, its speed, sensitivity,...

Claims

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Application Information

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IPC IPC(8): G01N21/45G02F1/03G02B1/11
Inventor 王品李勇明于彦涛夏宇任超梅林
Owner CHONGQING UNIV
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