Method for quickly detecting dibutyl phthalate in food

A fast technology of dibutyl phthalate, applied in measurement devices, instruments, fluorescence/phosphorescence, etc., can solve the problems of difficult to adapt to the rapid diagnosis of food industry, cumbersome sample processing, high monitoring costs, etc., to save detection time , the method is simple, the effect of short time-consuming

Inactive Publication Date: 2012-06-27
ANHUI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The most commonly used detection techniques are gas chromatography and liquid chromatography, but the monitoring costs are expensive, professional personnel are required ...

Method used

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  • Method for quickly detecting dibutyl phthalate in food
  • Method for quickly detecting dibutyl phthalate in food
  • Method for quickly detecting dibutyl phthalate in food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Establishment of the inhibition curve of dibutyl phthalate

[0054] (1) Coating steps:

[0055] The coating antigen DBAP-OVA at a concentration of 5μg / mL is coated with 96-well microtiter plate, 100μL per well, placed in the incubator and incubated at 37°C for 2h, then taken out of the incubator, shaken off the solution in the well, and then washed Wash with PBST and spin dry, wash 3-5min each time, wash 3 times in total.

[0056] (2) Closing steps:

[0057] Add 1% OVA solution, 150μL per well, to block the excess part that is not coated with antigen, to avoid non-specific adsorption of antibodies on the microtiter plate. After incubating at 37°C for 30 minutes, take it out of the incubator, shake off the solution in the well, and then wash as above.

[0058] (3) Competition steps:

[0059] Add 50μL of DBP standard solution and 50μL of fluorescent substance-labeled DBP antibody to each well to cause a competitive reaction. After incubating for 2.5 hours at 37°C, take it out of ...

Embodiment 2

[0068] Detecting the content of dibutyl phthalate in whole milk and adding standard recovery experiment

[0069] The pretreatment steps of whole milk: Weigh 2.0 g of whole milk solid powder (purchased from Wuhu supermarket), dissolve it in 10 mL of hot (70-90°C) distilled water, and wait for it to cool. Add 2 mL of absolute ethanol to the above 10 mL solution, shake for 5 min, and then centrifuge at 10,000 rpm at low temperature (0-4° C.) for 10 min. After centrifugation, take out the test tube, remove the upper fat layer, transfer the middle solution layer to a clean glass container, and adjust the pH of the solution to 7.0 with 1mol / L NaOH or 1mol / L HCl. Store at low temperature (0-4℃) )under.

[0070] 1. Detect the content of dibutyl phthalate in whole milk

[0071] except

[0072] (3) Competition steps:

[0073] Add 50μL of treated whole milk solution and 50μL of fluorescent substance-labeled DBP antibody solution to each well to cause a competitive reaction. After incubating fo...

Embodiment 3

[0081] Example 3: Detecting the content of dibutyl phthalate in liquid milk tea and adding standard recovery experiment

[0082] Pretreatment steps of liquid milk tea:

[0083] Add 2 mL of absolute ethanol to 10 mL of purchased liquid milk tea (purchased in a Wuhu supermarket) solution, shake for 5 min, and then centrifuge at 10,000 rpm at low temperature (0-4° C.) for 10 min. After centrifugation, take out the test tube, remove the upper fat layer, transfer the middle solution layer to a clean glass container, and adjust the pH of the solution to 7.0 with 1mol / L NaOH or 1mol / L HCl. Store at low temperature (0-4℃) )under.

[0084] 1. Detect the content of dibutyl phthalate in milk tea

[0085] (1) Coating steps:

[0086] The coating antigen DBAP-OVA at a concentration of 5μg / mL is coated with 96-well microtiter plate, 100μL per well, placed in the incubator and incubated at 37°C for 2h, then taken out of the incubator, shaken off the solution in the well, and then washed Wash with PB...

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PUM

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Abstract

The invention discloses a method for quickly detecting dibutyl phthalate, which comprises the following steps of: a, constructing an inhibitory curve; and b, sample detection. Compared with the prior art, the method is high in sensitivity, quick and high in specificity, adopts simple method and consumes less time, is applicable to the quick analysis of environment pollutant DBP (Dibutyl Phthalate) and is also significant for the determination of phthalate.

Description

Technical field [0001] The invention relates to a method for detecting dibutyl phthalate. Background technique [0002] Since April 2011, the incident of adding harmful plasticizers to food additives clouding agents in Taiwan has caused an uproar. Plasticizer is a kind of functional chemical that has solvation effect on polymer materials such as resin or plastic or rubber or adhesive. It is a kind of polymer material additives. The commonly used plasticizer is phthalate Formate. Research information shows that phthalates may have adverse effects on the environment and the human body. The European Union was the first international organization to restrict the use of phthalates. The EU Directive 2005 / 84 / EC lists six phthalate substances DEHP, DBP, BBP (butyl benzyl phthalate), DINP, DIDP (diisodecyl phthalate), DNOP ( Di-n-octyl phthalate) is restricted. The first three types of DEHP, DBP, and BBP shall not be used in children's toys and articles, and the content of each in pla...

Claims

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Application Information

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IPC IPC(8): G01N33/542G01N33/531G01N33/533G01N21/64
Inventor 张明翠胡玉嵘焦莉娟
Owner ANHUI NORMAL UNIV
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