Serologic detecting kit for potyvirus on sweet potato and detecting method thereof

A detection kit and detection method technology, applied in the field of bioengineering, can solve problems such as waste, time-consuming and labor-intensive, and achieve the effects of convenient detection, quality assurance, and cost-saving detection
CN102520185AInactive Publication Date: 2012-06-27HENAN ACAD OF AGRI SCI
7 Cites 7 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HENAN ACAD OF AGRI SCI
Publication Date
2012-06-27
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to a serologic detecting kit for potyvirus on a sweet potato and a detecting method thereof. The kit is divided into an upper layer and a lower layer, wherein reagent bottles are arranged on the upper layer; a push-pull drawer is arranged on the lower layer; a nitrocellulose film is placed into the drawer; and a specific antibody for the potyvirus, a mixed antibody for the potyvirus and a goat anti-rabbit IgG, NBT, BCIP and Tris-HCl solution marked with alkaline phospholipase are respectively filled in different reagent bottles. When a sample is detected, the reagent bottle containing the mixed reagent is used for detecting if the sample contains the potyvirus; other specific antibodies are used for detecting when a certain specific virus needs to be confirmed; the detection is convenient; and the cost is saved; and the detecting method is simple, direct, convenient and practical. The serologic detecting kit and the detecting method provided by the invention can be applied to the detection for the detoxified sweet potato stem seedling and all-level potatoes, and the quality and the yield-increasing effect of the detoxified sweet potatoes are ensured by quickly and efficiently detecting the potyviruses.
Need to check novelty before this filing date? Find Prior Art

Description

[0001] technical field

[0002] The invention relates to the technical field of bioengineering, in particular to a serological detection kit and a detection method for potato Y virus on sweet potatoes. Background technique

[0003] Sweet potato feather mottle virus ( Sweet potato feathery mottle virus , SPFMV), sweet potato latent virus ( Sweet potato latent virus , SPLV), sweet potato G virus ( Sweet potato virus G , SPVG) and sweet potato vein mosaic virus ( Sweet potato vein mosaic virus , SPVMV) are the main viruses that harm sweet potato, and all four viruses belong to the genus Potatovirus ( Potyvirus ), 4 kinds of viruses or mixed with other viruses often infect sweet potato, resulting in reduced yield, poor quality and degraded species of sweet potato, causing serious harm to sweet potato production. There is no particularly effective chemical control method for sweet potato virus disease at present. The most effective way to control virus disease is to culti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More