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Method for cultivating Poria cocos fruiting bodies

A cultivation method and the technology of poria cocos, which are applied in the field of cultivation, can solve problems such as reducing labor efficiency, increasing production costs, and long time for poria cocos, and achieve the effects of reducing labor consumption, preventing pollution, and accelerating fermentation speed

Inactive Publication Date: 2013-09-11
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the time for cultivating Poria cocos in solid medium is longer, and the quantity produced is less, which increases production cost and reduces labor efficiency.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A culture method for cultivating Poria cocos fruiting bodies, a method for obtaining Poria cocos fruiting bodies using a Poria cocos medium. The dosage is: 400mg of potatoes, 20g of bran, 1g of Poria cocos seed, 2g of yeast extract, 15g of glucose and 130mL of wheat grains;

[0025] Utilize the method for cultivating Poria cocos fruiting body with Poria cocos substratum, its culture method is:

[0026] Step 1: Add 130mL of boiled wheat seeds and 1g of Poria cocos mother seed into a 250mL conical flask, cultivate for 3 days at 28°C, crush the wheat seeds that have agglomerated during the cultivation process, and then Continue culturing for 3 days to obtain Poria cocos strains for subsequent use;

[0027] Step 2, add 400g of peeled potatoes and 20g of bran to 2000mL of water, cook the potatoes, filter to obtain the filtrate, and set aside;

[0028] Step 3: Add 2g of yeast extract and 15g of glucose to 1000mL of filtrate, mix well, sterilize at 121°C for 30 minutes, and ...

Embodiment 2

[0031] A culture method for cultivating Poria cocos fruiting bodies, a method for obtaining Poria cocos fruiting bodies using a Poria cocos medium. The addition amount is: 450mg of potatoes, 25g of bran, 2g of Poria cocos mother seed, 4g of yeast extract, 23g of glucose and 145mL of wheat grains;

[0032] Utilize the method for cultivating Poria cocos fruiting body with Poria cocos substratum, its culture method is:

[0033] Step 1. Add 145mL of boiled wheat seeds and 2g of Poria cocos mother seeds into a 250mL conical flask, cultivate for 3 days at 28°C, crush the wheat seeds that have agglomerated during the cultivation process, and then Continue culturing for 3 days to obtain Poria cocos strains for subsequent use;

[0034] Step 2, add 450g of peeled potatoes and 25g of bran to 2000mL of water, cook the potatoes, filter to obtain the filtrate, and set aside;

[0035] Step 3: Add 4g of yeast extract and 23g of glucose to 1000mL of filtrate, mix well, sterilize at 121°C for...

Embodiment 3

[0038] A culture method for cultivating Poria cocos fruiting bodies, a method for obtaining Poria cocos fruiting bodies using a Poria cocos medium. The dosage is: 500mg of potatoes, 30g of bran, 3g of Poria cocos seed, 5g of yeast extract, 30g of glucose and 160mL of wheat grains;

[0039] Utilize the method for cultivating Poria cocos fruiting body with Poria cocos substratum, its culture method is:

[0040] Step 1: Add 160 mL of boiled wheat seeds and 3 g of Poria cocos mother seeds into a 250 mL Erlenmeyer flask, and cultivate them for 3 days at 28°C, crush the wheat seeds that agglomerate during the cultivation process, and then Continue culturing for 3 days to obtain Poria cocos strains for subsequent use;

[0041] Step 2, add 500g of peeled potatoes and 30g of bran in 2000mL of water, cook the potatoes, filter to obtain the filtrate, and set aside;

[0042] Step 3: Add 5g of yeast extract and 30g of glucose to 1000mL of filtrate, mix well, sterilize at 121°C for 30 minut...

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PUM

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Abstract

A method for cultivating Poria cocos fruiting bodies belongs to a method of using a Poria cocos fruiting culture medium to obtain Poria cocos fruiting bodies. The Poria cocos fruiting culture medium comprises, by weight, 400-500mg of potato, 20-30mg of wheat bran, 2-5g of yeast extract, 1-3g of Poria cocos stock culture, 15-30g of glucose, and 130-160mL of wheat kernels. The method includes: adding a Poria cocos strain into the liquid inoculation culture medium; sending the liquid inoculation culture medium containing the Poria cocos strain into a shaker at 30 DEG C and 160rpm; culturing for 3 days by fermentation; taking out and allowing to stand for culture for 3 days at 28 DEG C; and culturing for 15-25days at the temperature 20-26 DEG C with illumination intensity 1600-2000lux to obtain the Poria cocos fruiting bodies. Using the method can quickly culture the Poria cocos fruiting bodies, and the method is simple.

Description

technical field [0001] The invention relates to a cultivation method, in particular to a cultivation method for cultivating poria cocos fruiting body. Background technique [0002] Poria ( Wolfiporia cocos ), also known as Poria, also known as Pine Poria, Pine Cypress Taro, Song Yu, etc., belongs to Basidiomycota, Phytomycetes, Non-Pleuromycetes, Polyporaceae, and Poria. It is a traditional precious medicinal and edible fungus in my country, which has the functions of nourishing the spleen and stomach, calming the nerves and promoting body fluid. [0003] Studies have reported that the fruiting body of Poria cocos is much smaller than other edible (medicinal) fungi, and the fruiting body is flat and flat on the surface of sclerotium, segment wood and soil, and is the sexual reproductive organ of Poria cocos. The fruit bodies of Poria cocos are of different sizes, initially white, and turn light yellow when mature or dry. Basidiospores are nearly cylindrical or rectangular...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04C05G3/00
Inventor 侯军胡梅张建祥黄治民李爱江
Owner HENAN UNIV OF SCI & TECH