Sterilization system for medium, sterilization method using same, and culture method for cordyceps militaris

A technology of sterilization system and cultivation method, which is applied in the field of cultivation of Cordyceps militaris, can solve the problems of inability to increase the biotransformation rate of the medium, low biotransformation rate of medium raw materials, low water content of the medium, etc., so as to improve the biotransformation rate , high practical value and application value, high drying effect

Active Publication Date: 2013-01-30
BEIJING ZHONGYI YUANDA MECHANICAL & ELECTRICAL EQUIP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Regardless of the medium raw materials such as rice, millet, wheat, sorghum, starch gelatinization will occur during the high temperature and high pressure sterilization process, and the raw materials in the medium will stick to each other, which will eventually lead to the small growth space of the mycelia in the medium raw materials or the mycelia cannot Growth phenomenon occurs, which eventually leads to very low bioconversion of medium raw material
Although the porosity in the medium can be increased to a certain extent by reducing the water content, due to the low water content in the medium, the fruiting bodies will stop developing early, and ultimately the biotransformation rate of the medium cannot be improved.

Method used

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  • Sterilization system for medium, sterilization method using same, and culture method for cordyceps militaris
  • Sterilization system for medium, sterilization method using same, and culture method for cordyceps militaris
  • Sterilization system for medium, sterilization method using same, and culture method for cordyceps militaris

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Embodiment 1: the sterilization system of culture medium

[0064] The sterilization system of the culture medium of the present invention includes: a steam generator 1 connected with an exhaust pipe and a sterilizing cabinet 2 (with an inner tank 2a and an outer tank 2b, and the inner tank and the outer tank are connected with an exhaust pipeline) , the exhaust pipe connected to the end of the sterilization cabinet 2 is divided into two branches, and a straight pipe (such as image 3 In the lower right corner, an exhaust switch is installed), and the other exhaust pipe (with an exhaust switch) is equipped with a vacuum tank 5 connected in sequence (its volume is 5 times the volume of the inner tank 2a of the sterilizer, and the vacuum tank is soaked In the cooling circulation water tank) and the water circulation vacuum pump 3 (equipped with a vacuum gauge), another pipeline is drawn from the exhaust pipeline connected to the inner tank 2a and the outer tank 2b of the s...

Embodiment 2

[0065] Embodiment 2: Utilize the sterilization system of embodiment 1 to sterilize the culture medium

[0066] specific method:

[0067] The method of this embodiment can not only sterilize the culture medium in the cultivation process of Cordyceps militaris, but also sterilize other solid medium, especially the granular material medium, which can not only remove free water, strengthen the drying degree, but also accelerate Material cooling.

[0068] (1) Put the medium into the sterilizer, and supply steam with a pressure of 0.11MPa to the sterilizer from the steam generator, and sterilize at 121°C for 20 minutes;

[0069] (2) Turn on the exhaust switch on the two branch roads to exhaust the steam. When the steam pressure drops to half (0.055MPa), close the exhaust switch on the straight exhaust pipe, and turn on the water circulation vacuum pump to force the exhaust steam;

[0070] (3) When the pressure indication of the water circulation vacuum pump is -0.05MPa, turn off ...

Embodiment 3

[0074] Embodiment 3: the improved culture method of Cordyceps militaris of the present invention

[0075] 1. Experimental time: April 2012

[0076] 2. Experimental materials:

[0077] (1) Strain: the Cordyceps militaris strain with the strain number HX-64 (from the Biology Center of Beijing Academy of Agriculture and Forestry Sciences);

[0078] (2) Strain solid medium: solid PDA slant, ingredients: 300 grams of potatoes, 20 grams of glucose, 20 grams of agar, 1000 ml of water, natural pH.

[0079] (3) Seed medium for liquid shake flasks: 250 grams of peeled potatoes (boiled juice), 8 grams of glucose, 2.5 grams of peptone, 0.6 grams of potassium dihydrogen phosphate, 1.2 grams of magnesium sulfate, 1000 ml of water, and natural pH.

[0080] (4) Nutrient solution for fruiting body production: 2.2% soybean, 1.5% glucose, 4.5% milk powder, 0.8% potassium dihydrogen phosphate, 0.06% magnesium sulfate, vitamin B 115mg / 1000ml, add water to 1000mL to make a nutrient solution , ad...

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Abstract

The invention relates to a sterilization system for a medium, a method for sterilization of a medium by using the sterilization system and a culture method for cordyceps militaris. The sterilization system for a medium comprises a steam generator and a sterilization cabinet connected through an exhaust pipeline, wherein the exhaust pipeline connected with the end of the sterilization cabinet is divided into two branch pipelines, one branch pipeline is straight, and a vacuum tank and a water circulating vacuum pump successively connected are installed on the other branch pipeline. According to the invention, voidage of the medium is increased, growth space of mycelia is enlarged, the medium has a fast cooling speed and a high drying degree, the purpose of improving biotransformation efficiency is finally achieved, production cost is reduced, which is favorable for popularization and application of corresponding technologies, and a high practical value and a high application value are obtained.

Description

technical field [0001] The invention relates to the technical field of cultivation of Cordyceps militaris, in particular to a sterilizing system for a medium, a method for sterilizing a medium by using the sterilizing system, and a method for cultivating Cordyceps militaris. Background technique [0002] Cordyceps militaris (L.Fr) Link, genus Ascomycota, Pyrenomycetes, Sphaeriales, Clavicipitaceae, Cordyceps (Fr.) Link) fungus. Cordyceps militaris is the model species of the fungus of the genus Cordyceps, and it is mostly parasitic on the larvae and pupae of various lepidopteran insects in nature. [0003] In recent years, studies have shown that the chemical composition and pharmacological effects of artificially cultivated Cordyceps militaris are similar to those of Cordyceps sinensis. Therefore, the development and application of Cordyceps militaris has a great potential market, and the artificial cultivation of Cordyceps militaris is a good way to solve the shortage of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L2/07A01G1/04
Inventor 胡健
Owner BEIJING ZHONGYI YUANDA MECHANICAL & ELECTRICAL EQUIP
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