Method for preparing high-performance bio-ironophore by using aspergillus niger

A technology of Aspergillus niger and biological iron, applied in microorganism-based methods, biochemical equipment and methods, immobilized on or in inorganic carriers, etc., can solve the difficulty of mass synthesis of microbial siderophores, limit the mass production of biological siderophores, problems such as poor biocompatibility, to achieve the effect of being conducive to amplification and industrialized production, good application value, and stable properties

Inactive Publication Date: 2007-05-30
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0004] The main problems in the application of siderophores are: most of the currently used siderophores are chemically synthesized siderophores, which have poor biocompatibility and are difficult to degrade, and are likely to cause environmental pollution after being used in large quantities
[0005] At present, there are reports of many microorganisms synthesizing siderophores at home and abroad [Annu.Rev.Microbiol.48:743-772,1994; Infect.Immun.59:3997-4000,1991; Clin.Microbio.Rev.Vol.12, No. 3, 394-404, 1999; Acta Microbiology, No. 1, 2000], but there are still some difficulties in using microorganisms to synthesize siderophores in large quantities
This is because the massive growth of microorganisms requires sufficient iron, but the synthesis of siderophores requires the induction of low iron conditions, and the feedback inhibition of iron on the synthesis of siderophores limits the mass production of biological siderophores.

Method used

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  • Method for preparing high-performance bio-ironophore by using aspergillus niger

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Experimental program
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Effect test

Embodiment 1

[0033]Aspergillus niger (Aspergillus niger) ATCC 6275 was inoculated on the slant of bran juice solid medium, and cultured in an incubator at 28°C for 5 days. After a large number of spores grew, the spores were washed with an appropriate amount of normal saline and collected. The spore filtrate was used Dilute with an appropriate amount of normal saline to adjust the spore concentration to 10 7 -10 8 per mL, placed in a sterile test tube and stored at 4°C for later use.

[0034] in:

[0035] The preparation method of the bran juice solid medium is: add 2% agar to the bran juice, sterilize at 121° C. for 20 minutes, and set aside; Dilute to 1L with distilled water;

[0036] The bacterial strain was inoculated in an Erlenmeyer flask containing bran juice liquid medium, the inoculum amount was 2% (v / v), 28°C, 170rpm shaking culture for 6 days, the siderophore activity could reach 95-100%, and the culture solution was collected.

[0037] in:

[0038] The preparation method o...

Embodiment 2

[0041] Aspergillus niger (Aspergillus niger) ATCC 10864 was inoculated on the slant of the bran juice solid medium, and cultured in an incubator at 28°C for 5 days. After a large number of spores grew, the spores were washed with an appropriate amount of normal saline and collected. The spore filtrate was used Dilute with an appropriate amount of normal saline to adjust the spore concentration to 10 7 -10 8 per mL, placed in a sterile test tube and stored at 4°C for later use.

[0042] The bacterial strain was inoculated in a Erlenmeyer flask containing potato glucose culture solution with an inoculation amount of 1% (v / v), cultured at 28° C. and 200 rpm for 7 days with shaking, and the siderophore activity was 90-95%, and the culture solution was collected.

[0043] Filtrate the fermented liquid and discard the cells. Ultrafiltration was performed with an ultrafiltration membrane with a molecular weight cut-off of 1KDa, and the filtrate was collected. The ultrafiltrate is ...

Embodiment 3

[0045] Aspergillus niger (Aspergillus niger) CGMCC 1067 was inoculated on the slant of bran juice solid medium, and cultured in an incubator at 28 °C for 4 days. After a large number of spores grew, the spores were washed with physiological saline and collected. The spore filtrate was treated with physiological Diluted with saline to adjust the spore concentration to 10 7 -10 8 each / mL, placed in a sterile test tube, stored at 4°C for later use;

[0046] in:

[0047] The preparation method of the bran juice solid medium is: add 2% agar to the bran juice, sterilize at 121° C. for 20 minutes, and set aside; Dilute to 1L with distilled water;

[0048] The above-mentioned Aspergillus niger spore suspension was inoculated into potato glucose liquid culture medium at an inoculation amount of 2% by volume, and cultured by shaking at 28° C. at 180 rpm. After 8 days, filter the culture with four layers of gauze, collect the fermentation broth, and discard it. Bacteria, the obtained...

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Abstract

The invention discloses a making method of high-effective biological ferric carrier through aspergillus niger, which comprises the following steps: (1) selecting bacterial; (2) allocating spore suspension; (3) fermenting liquid; (4) extracting ferment liquid; (5) refining ferric carrier. The invention improves the activity of ferric carrier, which possesses superior using value.

Description

technical field [0001] The invention relates to a method for preparing high-efficiency biological iron carriers by utilizing Aspergillus niger, which belongs to the field of microbial fermentation industry. Background technique [0002] Iron is an essential nutrient for almost all living cells and plays an important role in many biological processes. Fe 3+ Fe 2+ The interconversion of metal can generate redox potential, and it is a metal element necessary for the activity of some key metabolic enzymes. Although iron is the second most abundant metallic element in the earth's crust, most of it cannot be directly utilized by organisms. At neutral pH, the hydrated iron ions available to organisms in the environment, Fe 3+ concentration does not exceed 10 -18 mol L [J. Biological Chem. 270, 45:26723-26726, 1995]. Siderophore is a kind of low molecular weight (<1KDa) iron ion chelator produced by microorganisms to adapt to the physiological metabolism under low iron con...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P3/00C12N11/14C12R1/685
Inventor 卢雪梅孙红启张为灿赵越高培基
Owner SHANDONG UNIV
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