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Protease inhibitor BmSPI38 and preparation method and application thereof

A protease inhibitor, protease technology, applied in the direction of protease inhibitors, botanical equipment and methods, biochemical equipment and methods, etc., can solve the problem of unclear specific mechanism, improve the ability to resist pathogenic fungal invasion, The effect of high expression and simple operation

Active Publication Date: 2014-09-10
SOUTHWEST UNIV
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  • Abstract
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Problems solved by technology

[0005] Protease inhibitors play an important regulatory role in many physiological processes such as digestion, coagulation, fibrinolysis, complement cascade reaction, phenoloxidase cascade reaction, cell migration, hormone or peptide release, etc., but the specific mechanism of its action Not clear so far

Method used

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  • Protease inhibitor BmSPI38 and preparation method and application thereof
  • Protease inhibitor BmSPI38 and preparation method and application thereof
  • Protease inhibitor BmSPI38 and preparation method and application thereof

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Embodiment Construction

[0042] In order to make the purpose, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings. Experimental methods not indicating specific conditions in the examples are usually in accordance with conventional conditions, such as described in Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang et al., Science Press, 2002) conditions, or as recommended by the manufacturer.

[0043] The silkworm species used in the examples is Dazao, provided by the Silkworm Gene Resource Bank of Southwest University of China. The silkworm is fed with mulberry leaves or artificial feed, and the feeding conditions are: temperature 25°C, relative humidity 75% ± 5%, photoperiod 12 hours of light + 12 hours of darkness. The silkworm samples from the middle silk gland on the third day of the fifth instar and the silkworm on the seven...

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Abstract

The invention discloses a protease inhibitor BmSPI38, which is composed of the 23rd to 80th amino acids in SEQ ID No.2. BmSPI38 is very stable to temperature and pH, and can not only strongly inhibit subtilisin, proteinase K, and Beauveria bassiana The activity of wall degrading protease CDEP-1 and Aspergillus melissae protease, and can block the excessive and harmful melanization of silkworm induced by CDEP-1, can be used for the preparation of subtilisin protease inhibitors, and can also be used for the preparation of CDEP-1 induced The blocking agent of the insecticidal melanization reaction can also be used to cultivate silkworm strains with resistance to pathogenic fungi, and improve the ability of silkworms to resist the invasion of pathogenic fungi; in addition, the BmSPI38 prokaryotic expression method of the present invention is simple to operate, and the expression level high.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a protease inhibitor and its preparation method and application. Background technique [0002] The silkworm is a silk-producing insect with high economic value. However, silkworms are susceptible to various pathogenic microorganisms and other external factors to produce diseases. Entomopathogenic fungi such as Metarhizium anisopliae, Beauveria bassiana, Aspergillus flavus, and Aspergillus oryzae can cause highly infectious silkworm diseases. Once these pathogenic fungi spread in the silkworm area, it will cause great losses to the cocoon silk yield and quality, and even lead to the failure of the entire silkworm industry. Therefore, it is very important to take effective measures to control silkworm fungal diseases. [0003] Ultrastructural and histochemical evidences indicate that entomopathogenic fungi mainly penetrate the body wall of insects through the dual action of mechanical...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/81C12N15/15C12N15/63C12N15/70C12N1/21A61K38/57A61P31/10C12N15/85A01K67/04C12R1/19
Inventor 赵萍李游山夏庆友向仲怀
Owner SOUTHWEST UNIV
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