Oreochromis niloticus sex chromosome specific molecular marker

A Nile tilapia and molecular marker technology, applied in the field of genetic engineering, can solve the problems of inapplicability of genetic sex identification of Nile tilapia, undiscovered sex chromosome-specific molecular markers, and heavy workload of artificial breeding, etc., reaching The effect of reducing the cost of breeding, accelerating market demand and low cost

Inactive Publication Date: 2012-07-04
SOUTHWEST UNIVERSITY
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Problems solved by technology

[0005] The traditional production of all-male fish is mainly through artificial breeding, androgen-induced sex reversal, etc., but these methods have defects, such as artificial breeding workload, poor accuracy; androgen-induced sex reversal has food safety problems, etc.
In addition, there are the following research reports: Lee et al. used group segregation analysis to find 10 SSR markers linked to the sex of the tilapia phenotype. The identification of...

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  • Oreochromis niloticus sex chromosome specific molecular marker
  • Oreochromis niloticus sex chromosome specific molecular marker
  • Oreochromis niloticus sex chromosome specific molecular marker

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Embodiment Construction

[0022]Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. Experimental methods not indicating specific conditions in the preferred embodiments are generally in accordance with conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, Science Press, 2002) conditions, or as recommended by the manufacturer.

[0023] 1. Acquisition of sex chromosome-specific molecular markers in Nile tilapia

[0024] 1. Screening and cloning of Nile tilapia sex chromosome-specific AFLP markers

[0025] (1) Genomic DNA extraction

[0026] Cut 10 mg of Nile tilapia fin rays, put it into 600 μl of lysate [10mM Tris-HCl (pH8.0)+100mM EDTA (pH8.0)+100mM NaCl+5mg / ml SDS] for homogenization, and add the final concentration of 20mg / ml proteinase K and 100μg / ml final concentration of ribonuclease A (RNaseA), digested in a water bath ...

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Abstract

The invention belongs to the technical field of genetic engineering and particularly relates to an oreochromis niloticus sex chromosome specific molecular marker, namely an X chromosome specific molecular marker NtX1, which has a nucleotide sequence represented by SEQ ID No.3; through polymerase chain reaction(PCR) amplification, the X chromosome specific molecular marker NtX1 and a Y chromosome specific molecular marker NtY1 and/or a Y chromosome specific molecular marker NtY2 can be used to differentiate YY supermale oreochromis niloticus, XY male oreochromis niloticus and XX female oreochromis niloticus and promote the mass production of all-male oreochromis niloticus fry through a YY supermale oreochromis niloticus breeding route; therefore, the breeding output of oreochromis niloticus is increased obviously, the breeding cost is reduced, and the economic benefit is increased.

Description

[0001] This application is a divisional application with the application number 201010501061.8, the application date is 2010-10-09, and the invention name is "Nile tilapia sex chromosome specific molecular marker and genetic sex identification method". technical field [0002] The invention belongs to the technical field of genetic engineering, and relates to fish sex chromosome-specific molecular markers and a genetic sex identification method, in particular to tilapia sex chromosome-specific molecular markers and a genetic sex identification method. Background technique [0003] my country is rich in fish resources, many of which have gender differences in growth rate, and some species of females grow faster (40-100%) than males, such as flounder, semi-smooth tongue sole, star flounder, Southern catfish, etc.; some species of male individuals grow faster than female individuals (over 40%), such as yellow catfish, tilapia, etc. Carrying out research on genetic sex identifica...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 王德寿孙运侣曾圣叶凯黄宝锋
Owner SOUTHWEST UNIVERSITY
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