Method for quickly detecting microorganisms

A detection method and microbial technology, applied in the direction of color/spectral characteristic measurement, fluorescence/phosphorescence, material excitation analysis, etc., can solve problems such as insurmountable drift interference, drift of microbial growth curve, and influence on the accuracy of detection results, etc., to improve Applicability, effect of improving accuracy

Active Publication Date: 2012-07-04
FOCUSED PHOTONICS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] 2. Due to the background interference caused by free enzymes or other compounds in the sample, the microbial growth curve obtained by detection drifts, and the method of determ...

Method used

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  • Method for quickly detecting microorganisms
  • Method for quickly detecting microorganisms
  • Method for quickly detecting microorganisms

Examples

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Embodiment 1

[0044] A rapid detection method for microorganisms, used to detect E. coli in water samples, comprising the following steps:

[0045] A. Setting Thresholds and Modeling Steps

[0046] Four Escherichia coli standard samples (standard sample a, standard sample b, standard sample c and standard sample d, the concentrations C were 95.30cfu, 1.34×10 3 cfu, 1.22×10 4 cfu and 2.00×10 6 cfu) were added to the culture solution respectively, and the Escherichia coli in each standard sample grew in the culture solution respectively, and the culture solution contained the reaction substrate 4-methylumbelliferone-β-D glucuronide (MUG), and the MUG were respectively React specifically with the metabolic indicator β-D-glucuronidase produced by the growth of Escherichia coli to generate fluorescent signals (excitation wavelength is 365nm, emission wavelength is 450nm), which are detected separately to obtain the growth of each standard sample curves, such as image 3 shown.

[0047] At t...

Embodiment 2

[0054] A rapid detection method for microorganisms, used to detect total coliforms in water samples, comprising the following steps:

[0055] A. Setting Thresholds and Modeling Steps

[0056] Take 4 total coliform standard samples (standard sample a, standard sample b, standard sample c and standard sample d, the concentration C of which is 95.30cfu, 1.34×10 3 cfu, 1.22×10 4 cfu and 2.00×10 6 cfu) were added to the culture solution respectively, and the total coliform bacteria in each standard sample grew in the culture solution respectively, and the culture solution contained 4-methylumbelliferone-β-D-galactocuronide (MUGal), and MUGal was respectively It reacts specifically with the metabolic indicator β-D-galactosidase produced by the growth of each total coliform group, and generates a fluorescent signal (excitation wavelength is 365nm, emission wavelength is 450nm), which are detected separately to obtain the growth of each standard sample. curves, such as Figure 4 s...

Embodiment 3

[0065] A rapid detection method for microorganisms, used to detect staphylococci in food samples, comprising the following steps:

[0066] A. Setting Thresholds and Modeling Steps

[0067] The 7 staphylococcal standard samples (standard sample 1, standard sample 2, standard sample 3, standard sample 4, standard sample 5, standard sample 6 and standard sample 7, the concentration C is 24.36cfu, 198.48cfu, 2001cfu, 14506cfu respectively , 15742cfu, 16668cfu and 1986045cfu) were added into the culture solution respectively, and the staphylococci in each standard sample grew in the culture solution respectively, and the culture solution contained reaction substrates Boc-le and u-Gly-Arp-p, respectively with each The metabolic indicator coagulase produced by the growth of Staphylococcus in the standard sample reacted specifically to produce colored p-nitroa-niline (PNA). Such a growth curve, such as Figure 5 shown.

[0068] Set the threshold value according to the slope value o...

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Abstract

The invention relates to a method for quickly detecting microorganisms, which includes the following steps: a, modeling: standard microorganisms are added in a culture solution to grow in the culture solution, signals generated during the growth of the standard microorganisms are detected, and the detection time of the standard microorganisms are recorded when the detection signals are reduced at the end of the lag growth phase of the standard microorganisms; and the standard model of the concentration and the detection time of the microorganisms is obtained as per the concentration and the detection time of the standard microorganisms; and b, detection: a to-be-detected microorganism is added in the culture solution to grow in the culture solution, signals generated during the growth of the to-be-detected microorganism is detected, and the detection time of the to-be-detected microorganism is recorded when the detection signals are reduced at the end of the lag growth phase of the to-be-detected microorganism; and the concentration of the to-be-detected microorganism is obtained as per the detection time and the standard model of the to-be-detected microorganism. The invention has the advantages that the method is quick and accurate and has wide application range and the like, and can effectively eliminate the background interference.

Description

technical field [0001] The invention relates to the detection of microorganisms, in particular to a rapid detection method for microorganisms. Background technique [0002] Microbiological detection is an important part of environmental or food safety assessment. Among them, the total number of bacteria, coliform bacteria and pathogenic microorganisms are internationally recognized health detection indicators, which are important scientific basis for judging whether the environment or food is safe. Microbial detection can correctly evaluate the degree of microbial contamination of the environment or food, provide scientific basis for various health management work, and provide important scientific guidance for the targeted prevention and treatment of related diseases to ensure people's health; It is of great significance to the environment or food quality, to avoid economic losses, to ensure exports, etc. [0003] The traditional microbial detection method is the culture me...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N21/31
Inventor 赵鹏忻鼎丞苏东霞
Owner FOCUSED PHOTONICS
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