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Quality detection method for senecio cannabifolius less extract

A detection method and the technology of rejuvenation grass, which are applied in the field of quality detection of traditional Chinese medicine to achieve the effects of high precision, good stability, accuracy and authenticity.

Active Publication Date: 2015-01-21
吉林益民堂制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, in the production and circulation of traditional Chinese medicine, there is no quality control method that can comprehensively and comprehensively reflect the quality variation of traditional Chinese medicine products, and can effectively control the quality of the whole process

Method used

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  • Quality detection method for senecio cannabifolius less extract
  • Quality detection method for senecio cannabifolius less extract
  • Quality detection method for senecio cannabifolius less extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Constructing the HPLC Fingerprint of Herba Resurrection Extract

[0039] 1. Instruments and reagents

[0040]1.1 Instrument: Shimadzu LC-2010AHT high performance liquid chromatography (quaternary pump, online degasser, column thermostat (column temperature 28°C), automatic sampler, UVD detector); BS210S electronic balance; chromatographic column: Agilent ZORBAX SB-C 18 (5μm, 4.6×250mm).

[0041] 1.2 Reagents: chlorogenic acid reference substance, caffeic acid reference substance (provided by China Pharmaceutical and Biological Products Identification Institute). The reagents used for liquid chromatography analysis are chromatographically pure, the rest of the reagents are analytically pure, and the water is ultrapure water.

[0042] 2. High performance liquid chromatography

[0043] 2.1 Preparation of reference solution: Accurately weigh the appropriate amount of reference substances of chlorogenic acid and caffeic acid, weigh them accurately, add methanol...

Embodiment 2

[0047] Example 2 Constructing the HPLC Standard Fingerprint of Herba Resurrection Extract

[0048] Get 12 batches of herbal extracts (respectively from 3 batches of Changbai Mountain Medicinal Materials Institute, 2 batches of Heilongjiang Medicinal Materials Institute, 3 batches of Jilin Siping Medicinal Materials Institute, 2 batches of Liaoning Medicinal Materials Institute, 2 batches of Inner Mongolia Medicinal Materials Institute), and carry out according to the conditions of Example 1 Detect, obtain the HPLC collection of illustrative plates of 12 batches of samples, such as figure 2 shown. Through the comparison of 12 batches of HPLC chromatograms, the software "Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System" recommended by the Chinese Pharmacopoeia Commission was used to evaluate the similarity of the HPLC chromatograms, determine the common characteristic peaks, and obtain the standard fingerprints of Herba Fenhunen.

[0049] In the standa...

Embodiment 3

[0054] The quality detection method of embodiment 3 Revenant Herb Extract

[0055] Get 20 batches of Herba Herbs Extract samples (four batches each of Changbai Mountain's annual home-grown medicinal materials, Changbai Mountain's wild medicinal materials, Heilongjiang Daxing'anling wild medicinal materials, Jilin Siping wild medicinal materials, and Inner Mongolian wild medicinal materials, all plucked in August and dried), according to the examples The conditions of 1 were operated in the same way to obtain the sample fingerprints of Herba Herbs Extract, and the standard fingerprints and sample fingerprints of Herbs Herbs Extract were analyzed by using the Chinese medicine chromatographic fingerprint similarity evaluation system software, and the similarity was greater than 0.95 as qualified product.

[0056] The test results show that the wild medicinal materials in Changbai Mountain, the wild medicinal materials in Daxing’anling, Heilongjiang, the wild medicinal materials i...

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Abstract

The invention relates a construction method for a senecio cannabifolius less extract HPLC (High Performance Liquid Chromatography) fingerprint spectrum. The method comprises the steps of: (1) preparing reference substance solution; (2) preparing sample solution; (3) creating chromatograph conditions, wherein a chromatographic column takes octadecylsilane bonded silica as a filling agent, and the mobile phase uses the mixed solution prepared by acetonitrile and 0.4% of phosphoric acid solution by a ratio of 7-9 to 91-93; and (4) measuring to obtain the senecio cannabifolius less extract fingerprint spectrum. The invention also relates to a quality detection method for the senecio cannabifolius less extract and a standard fingerprint spectrum. Through the method provided by the invention, the quality of the senecio cannabifolius less extract can be effectively characterized, the quality of products can be monitored, the characteristics of simplicity and convenience in the method, good stability, high precision and good reproducibility are achieved, and the authenticity as well as the advantages and disadvantages of the products can be rapidly and accurately identified.

Description

technical field [0001] The invention relates to a method for detecting the quality of traditional Chinese medicines, in particular to a method for constructing HPLC fingerprints of herb extract and a method for detecting the quality of herb extract. Background technique [0002] The Chinese herbal medicine Huanhuncao is mainly produced in Changbai Mountains, Daxinganling Mountains and other places in my country. The whole herb on the ground is used as medicine, which has the functions of clearing away heat and detoxification, dispersing blood, reducing swelling, lowering Qi and dredging the meridians. For the effect of blood stasis distending pain, bruises. At the same time, it also has antibacterial effect, and it has a significant curative effect in clinical treatment of pulmonary heart disease, chronic bronchitis, and acute lung infection. In addition, it has the effects of preventing and treating influenza, moisturizing the lungs, and improving human immunity. Due to ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88A61K36/28A61P11/00
Inventor 丁爱英罗敏崔业波李艳茹徐阳马晓静
Owner 吉林益民堂制药有限公司