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Immune chromatography test strip for detecting cystic echinococcosis and alveolar echinococcosis and preparation method

A technology for alveolar echinococcosis and cystic echinococcosis, which is applied in the field of bioengineering, can solve the problems of complex detection methods for cystic echinococcosis and alveolar echinococcosis, and the inability to diagnose early, and achieve easy industrial production, Low cost, high sensitivity and specificity

Active Publication Date: 2012-07-04
SHANGHAI NEW JIEER CLEANING PRODS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The purpose of the present invention is to provide an immunochromatographic test strip and preparation method for detecting cystic echinococcosis and alveolar echinococcosis. The analysis test strip and the preparation method should solve the technical problems that the methods for detecting cystic echinococcosis and alveolar echinococcosis in the prior art are complicated and cannot be diagnosed early

Method used

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  • Immune chromatography test strip for detecting cystic echinococcosis and alveolar echinococcosis and preparation method
  • Immune chromatography test strip for detecting cystic echinococcosis and alveolar echinococcosis and preparation method
  • Immune chromatography test strip for detecting cystic echinococcosis and alveolar echinococcosis and preparation method

Examples

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Effect test

Embodiment 1

[0050] An immunochromatographic test strip for detecting cystic echinococcosis and alveolar echinococcosis of the present invention includes a backboard 8, and one end of the upper side of the backboard 8 is provided with a sample pad 1, and the The other end of the upper side of the backboard 8 is provided with an absorbent pad 4, and a cellulose film 3 is arranged between the sample pad 1 and the absorbent pad 4, and the absorbent pad 4 and the cellulose membrane 3 are tightly connected , a gold standard pad 2 is arranged between the sample pad 1 and the cellulose membrane 3, the sample pad 1, the gold standard pad 2 and the cellulose membrane 3 are tightly connected, and the A quality control line 7 is set on the cellulose membrane 3 away from the end of the gold standard pad 2, and a first detection line 6 and a second detection line are set on the cellulose membrane 3 between the quality control line 7 and the gold standard pad 2 5. The first detection line 6 is composed ...

Embodiment 2

[0054] Example 2 Preparation of immunochromatographic test strips for detecting cystic echinococcosis and alveolar echinococcosis

[0055] 1. Preparation of purified crude antigen of cystic echinococcosis

[0056] Take fresh goat liver cyst fluid from Xinjiang, centrifuge at 1000g for 30min, suck the supernatant into a dialysis bag, dialyze overnight at 4°C with 5mM acetic acid buffer solution of pH 5.0, centrifuge at 50000g for 30min, discard the supernatant to remove albumin . The precipitate was dissolved in 0.2M PBS with pH 8.0, saturated ammonium sulfate was added to 40%, and the precipitated globulin was removed by centrifugation to obtain the crude antigen of purified cyst fluid.

[0057] 2. The recombinant alveolar echinococcosis antigen Em18 was prepared as follows

[0058] 2.1 Cloning and transformation of the target gene

[0059]The protoscole of Echinococcus multilocularis from natural infection in sheep liver was collected in Xinjiang. The total RNA of the pro...

Embodiment 3

[0090] Embodiment 3, the preparation of the immunochromatography test strip of the immunochromatography kit detecting cystic echinococcosis and alveolar echinococcosis

[0091] The colloidal gold-labeled detection probe was prepared with goat anti-human IgG purchased from Shanghai Yeli Biotechnology Co., Ltd., and the quality control line was coated with rabbit anti-goat IgG purchased from Jiening Biotechnology Co., Ltd. The other steps were the same as in Example 1.

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Abstract

The invention belongs to the field of bioengineering and discloses an immune chromatography test strip for detecting cystic echinococcosis and alveolar echinococcosis, which comprises a back plate. A sample cushion is arranged at one end of the upper side of the back plate, and a water absorption cushion is arranged at the other end of the upper side of the back plate, a cellulose membrane is arranged between the sample cushion and the water absorption cushion, and a gold-labeled cushion is arranged between the sample cushion and the cellulose membrane. The cellulose membrane is provided with a first detecting line, a second detecting line and a quality control line. The first detecting line contains a purified thick antigen specific to the cystic echinococcosis, the second detecting line contains a recombinant antigen specific to the alveolar echinococcosis, and the quality control line contains antibodies or antiantibodies capable of being in specificity combination with a colloid gold-labeled probe. The invention further provides a preparation method of the immune chromatography test strip for detecting the cystic echinococcosis and the alveolar echinococcosis. The immune chromatography test strip has the advantages of simplicity, sensitivity, specificity and rapidity and being suitable for clinic and on-site use.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to an immunochromatographic test strip and a preparation method thereof, in particular to an immunochromatographic test strip and a preparation method for detecting cystic echinococcosis and alveolar echinococcosis. Background technique [0002] Echinococcosis, also known as Hydatid disease or Hydatidosis, is a zoonotic parasitic disease caused by the larvae of Echinococcus tapeworms parasitic in humans and animals, and can infect human echinococcus There are four species of tapeworms: Echinococcus granulosus (Eg), Echinococcus multilocularis (Em), E. oligarthrus and E. vogeli. ). The adult and larval stages of these four Echinococcus species are different in morphology and can cause different types of echinococcosis. There are two types of echinococcosis in my country, namely, cystic echinococcus-cosis (CE), also known as cystic echinococcosis, and alveolar echinococcosis (AE), also...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/577G01N33/558
Inventor 汪俊云石锋高春花杨玥涛胡薇
Owner SHANGHAI NEW JIEER CLEANING PRODS
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