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Application of sodium hydrogen sulfide aqueous solution in improving heavy metal stress resistance of plant

A technology of sodium hydrosulfide and aqueous solution, applied in the field of known compounds, to achieve the effects of easy promotion, low cost, and improved ability to resist heavy metal stress

Inactive Publication Date: 2012-07-11
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Some people have studied that NO can be used to promote the synthesis of glutathione in plants to improve the ability of plants to resist heavy metals, but because NO can support combustion, is toxic, and is irritating, etc. Disadvantages cannot be widely used in production practice

Method used

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  • Application of sodium hydrogen sulfide aqueous solution in improving heavy metal stress resistance of plant
  • Application of sodium hydrogen sulfide aqueous solution in improving heavy metal stress resistance of plant
  • Application of sodium hydrogen sulfide aqueous solution in improving heavy metal stress resistance of plant

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Effect test

Embodiment 1

[0018] Embodiment 1. When in use, weigh sodium hydrosulfide monohydrate (NaHS·H 2 O) Add water to prepare a 100 μmol / L aqueous solution, and the treatment solution is ready-to-use. Spray on living Arabidopsis, extract RNA after 1, 3 and 6 hours, and reverse transcription, use ACTIN As an internal reference, observe the expression of glutathione synthase gene 1 and 2, such as figure 1 As shown, compared with the absence of inducer, gene expression was greatly increased.

Embodiment 2

[0019] Embodiment 2. When in use, weigh sodium hydrosulfide monohydrate (NaHS·H 2 O) Add water to prepare a 100 μmol / L aqueous solution, and the treatment solution is ready-to-use. Spray on live Arabidopsis thaliana, randomly pick plants at different times, and use the kit to measure the content of glutathione, which is significantly increased compared with the control.

Embodiment 3

[0020] Embodiment 3. The application experiment of sodium hydrosulfide aqueous solution in improving the resistance of plants to heavy metal stress: Arabidopsis was sprayed with distilled water (control) and 100 μmol / L NaHS solution respectively, and the growth of plants on the substrate with heavy metals was observed. The results are shown in image 3 . Depend on image 3 It can be seen from A that the sprayed plant leaves are in good growth condition, and can grow normally on the substrate containing heavy metals; there is no obvious effect on the dry weight ( image 3 B).

[0021]

[0022] From the above results, it can be seen that the present invention can significantly increase the expression of plant glutathione synthase gene 1 and 2, increase the concentration of glutathione, enhance the ability of plants to resist heavy metal stress, and protect the normal growth of plants.

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Abstract

The invention provides novel application of sodium hydrogen sulfide aqueous solution, particularly provides application of sodium hydrogen sulfide aqueous solution in improving heavy metal stress resistance of a plant. The sodium hydrogen sulfide aqueous solution has a concentration of 10-500 mumol / L, preferably 50-100mumol / L. The sodium hydrogen sulfide aqueous solution sprayed on plant left surface can induce high expression level of glutathione synthetase gene 1 (GSH1) and glutathione synthetase gene 2 (GSH2), so as to achieve the effect of synthesizing a large amount of glutathione (GSH). Meanwhile, the sodium hydrogen sulfide aqueous solution can supplement sulfur (S) required by the plant, so as to provide guarantee for heavy metal stress resistance of the plant.

Description

technical field [0001] The present invention relates to a new application of a known compound, specifically sodium hydrosulfide (NaHS) as a new method for inducing the expression of glutathione synthase gene in plants and improving the synthesis of glutathione in plants to improve the resistance of plants to heavy metal stress. use. Background technique: [0002] With the rapid development of modern industry and agriculture, heavy metal pollutants enter the soil through various channels, resulting in soil heavy metal pollution of varying degrees worldwide. This pollution directly affects soil quality, water quality, crop growth, agricultural product yield, quality, etc., and causes harm to human health through the food chain. Therefore, fully understanding the long-term, hidden, irreversible and inability to completely decompose or disappear the characteristics of heavy metal pollution in soil, and to carry out the treatment of heavy metal pollution has become a problem th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/06
Inventor 裴雁曦乔增杰张丽萍金竹萍田保华
Owner SHANXI UNIV
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