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Nasopharyngeal carcinoma targeted magnetic resonance contrast agent and preparation method thereof

A technology of magnetic resonance contrast agent and nasopharyngeal carcinoma, which is applied in the field of medical biomaterials, can solve the problems of weak tumor active targeting aggregation function and short half-life, and achieve high relaxation performance and good safety effects

Active Publication Date: 2013-03-27
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage is that it is easily phagocytized by the reticuloendothelial system and immune phagocytes in the body, so the half-life is short and the active targeting and aggregation function of tumors is weak

Method used

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  • Nasopharyngeal carcinoma targeted magnetic resonance contrast agent and preparation method thereof
  • Nasopharyngeal carcinoma targeted magnetic resonance contrast agent and preparation method thereof
  • Nasopharyngeal carcinoma targeted magnetic resonance contrast agent and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Mix 0.5mmoL ferrous chloride and 1.2mmoL ferric chloride aqueous solution, add 15mL sodium dodecylbenzenesulfonate (DBS) solution (25mM) prepared in advance, after the solution becomes milky white, add 75mL toluene, stir at high speed Allow the solution to mix evenly for 30 minutes, then add dropwise a 2.5M NaOH solution to adjust the pH to 8-9, and continue stirring for 1 hour. Transfer the mixed solution into a separatory funnel and let it stand for stratification. It can be seen that the particles are dispersed in the upper layer of toluene solution. The water layer is separated, and the upper layer of black liquor is transferred into a three-necked bottle. After distilling off the water and part of the toluene in the solution, it was refluxed for half an hour, and then dissolved in water and mixed with 3-aminopropyltriethoxysilane at a molar ratio of 0.1:1. The mixture was refluxed under pure argon for half an hour to crystallize the magnetite, and the nanoparticles...

Embodiment 2

[0041] Mix 0.5mmoL ferrous chloride and 1.2mmoL ferric chloride aqueous solution, add 15mL sodium dodecylbenzenesulfonate (DBS) solution (25mM) prepared in advance, after the solution becomes milky white, add 75mL toluene, stir at high speed Allow the solution to mix evenly for 30 minutes, then add dropwise a 2.5M NaOH solution to adjust the pH to 8-9, and continue stirring for 1 hour. Transfer the mixed solution into a separatory funnel and let it stand for stratification. It can be seen that the particles are dispersed in the upper layer of toluene solution. The water layer is separated, and the upper layer of black liquor is transferred into a three-necked bottle. After distilling off the water and part of the toluene in the solution, it was refluxed for half an hour, and then dissolved in water and mixed with 3-aminopropyltriethoxysilane at a molar ratio of 0.1:1. The mixture was refluxed under pure argon for half an hour to crystallize the magnetite, and the nanoparticles...

Embodiment 3

[0043] Mix 0.5mmoL ferrous chloride and 1.2mmoL ferric chloride aqueous solution, add 15mL sodium dodecylbenzenesulfonate (DBS) solution (25mM) prepared in advance, after the solution becomes milky white, add 75mL toluene, stir at high speed Allow the solution to mix evenly for 30 minutes, then add dropwise a 2.5M NaOH solution to adjust the pH to 8-9, and continue stirring for 1 hour. Transfer the mixed solution into a separatory funnel and let it stand for stratification. It can be seen that the particles are dispersed in the upper layer of toluene solution. The water layer is separated, and the upper layer of black liquor is transferred into a three-necked bottle. After distilling off the water and part of the toluene in the solution, it was refluxed for half an hour, and then dissolved in water and mixed with 3-aminopropyltriethoxysilane at a molar ratio of 0.1:1. The mixture was refluxed under pure argon for half an hour to crystallize the magnetite, and the nanoparticles...

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Abstract

The invention discloses a nasopharyngeal carcinoma targeted magnetic resonance contrast agent and a preparation method thereof. The method comprises the following steps: synthesizing superparamagnetic Fe3O4 with grain diameter of between 10 and 15nm by adopting a chemical coprecipitation method, coating with APTES ((3-aminopropyl)triethoxysilane) or connecting superparamagnetic Fe3O4 to 10-15nm Fe3O4 to carry out surface amination on Fe3O4 to obtain Fe3O4-APTES surface-modified micro-particles; and connecting Fe3O4-APTES and EB (Epstein-Barr) virus latent membrane protein 1 monoclonal antibody (LMP1, Clone CS.1-4) with polyethylene glycol (PEG) as a connecting arm to obtain Fe3O4-APTES-PEG-LMP1, Clone CS.1-4 colloidal solution with stable dispersion. Compared with a conventional contrast agent, the contrast agent has the advantages of low toxicity, high stability, good biocompatibility, high sensitivity and good specific targeting property to LMP1<+>-nasopharyngeal carcinoma, and can be used for nasopharyngeal carcinoma screening and specific magnetic resonance diagnosis.

Description

technical field [0001] The invention relates to the field of medical biomaterials, especially a kind of LMP1 + - Nasopharyngeal carcinoma targeting magnetic resonance contrast agent and preparation method thereof. Background technique [0002] Tumor is one of the major diseases affecting human health today. A report by the World Health Organization shows that with the changes in the global human living environment, the incidence of global malignant tumors will increase from the current 10 million people / year to 15 million people / year. has become the number one killer threatening human health. In my country (especially South China), the incidence of nasopharyngeal carcinoma has been on the rise in recent years, and 80% of nasopharyngeal carcinoma cases in the world occur in China. [0003] The pathological type of nasopharyngeal carcinoma is mainly non-keratinizing undifferentiated carcinoma, which is prone to cervical lymph nodes and distant metastasis; in the first course...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/16
Inventor 王维容鹏飞刘晟周科朝曾文彬邹蟠张声旺
Owner CENT SOUTH UNIV
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