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Halophilic aspergillus sp.F1 and application thereof

A technology of Aspergillus halophilus and mycelium, which is applied to Aspergillus halophilus F1 and its application fields, which can solve the problems of high price, difficulty in artificial synthesis, complex structure, etc., and achieve high yield, reduce pollution, and low fermentation and preparation costs Effect

Inactive Publication Date: 2012-07-11
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complex structure of Cytochalasin E, it is difficult to synthesize artificially, and most of the cytochalasin tool compounds are imported products, and the commodity price is relatively high. Therefore, it is particularly necessary to develop high-yield Cytochalasin E microbial strains with independent intellectual property rights in my country.

Method used

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  • Halophilic aspergillus sp.F1 and application thereof
  • Halophilic aspergillus sp.F1 and application thereof
  • Halophilic aspergillus sp.F1 and application thereof

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Embodiment Construction

[0019] The compound of the present invention can be obtained through microbial fermentation and culture. Firstly, the fermented product containing the compound is obtained, and then separated and purified from the fermented product by silica gel column chromatography, recrystallization and Sephadex LH-20 gel column chromatography.

[0020] Halophilic Aspergillus sp.F1 (Aspergillus sp.F1), preserved in the China Center for Type Culture Collection (in Wuhan University, China), the preservation number is: CCTCC NO: M 2011277, and the preservation date is August 4, 2011. The mycelium of this strain is white, rich in brown spores, reproduces by ascospores and mycelium division, produces brown pigment, can tolerate 18% high-salt environment, belongs to medium halophilic bacteria, and has a sea salt concentration of 3-6 %(w / v) is growing vigorously.

[0021] Method for Cytochalasins produced by Aspergillus halophilus F1:

[0022] 1. Fermentative production and separation and purific...

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Abstract

The invention relates to the biotechnological field, in particular to a halophilic aspergillus sp.F1 and an application thereof. The aspergillus sp.F1 is preserved in the China Center for Type Culture Collection (CCTCC)(in Wuhan university, China), the preservation number is: CCTCC NO.M 2011277, and the preservation date is August 4, 2011. Pure products of 1.5 grams of Rosellichalasin and 2.0 grams of Cytochalasin E are obtained from 40 liters of fermentation products after fermentation of strains, and product purities reach over 99%.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a halophilic aspergillus F1 and its application. Background technique [0002] Cytochalasins are a class of toxins produced by fungal metabolism, and some of them have significant biological activities, such as inhibiting mammalian cell division, inhibiting HIV-1 protease, antibacterial and antitumor activities. Because of its ability to change the microstructure of cells, cytochalasin has become a common tool in the study of cell microstructure and function in cell biology. Activity. [0003] Cytochalasin compounds Rosellichalasin and Cytochalasin E have certain inhibitory effects on Escherichia coli, Pseudomonas and Candida albicans respectively. Cytochalasin E is a cytochalasin compound that is widely used at home and abroad. As a common tool for the study of animal and plant cell structure and function, it shows unique selection diversity. In particular, it is an angiogenesis i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P17/18C07D491/20C12R1/66
Inventor 林秀坤肖琳吴宁刘明刘海洲魏鉴腾赵进王惠王凤霞郭颖
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI