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Non-contact alternating current electrospray ionization device and method

A non-contact, spray device technology, applied in the direction of ion source/gun, particle separator tube parts, test sample preparation, etc., can solve the problems of increasing process, complex structure of electrospray ion source, etc., and achieve processing Easy, simple structure, and the effect of overcoming the matrix salt effect

Active Publication Date: 2012-07-11
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] For the above devices, since the sample solution is inside the capillary, it is necessary to add an additional interface to apply the spray voltage to the spray needle, which makes the structure of the electrospray ion source relatively complicated, and also adds additional components during the preparation process. process

Method used

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  • Non-contact alternating current electrospray ionization device and method
  • Non-contact alternating current electrospray ionization device and method
  • Non-contact alternating current electrospray ionization device and method

Examples

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Embodiment 1

[0034] Implementation example 1: The electrode 2 is an iron wire (length 30mm, diameter 5mm) connected to the AC power supply 3, the other end of the AC power supply 3 is grounded, the peak-to-peak voltage of the applied voltage is 0.6kV, and the frequency is 250kHz. The nebulizer pressure was adjusted to 100 psi. Sample solution: myoglobin is dissolved in methanol / water / acetic acid (8:2:0.1, v:v) solution, ionized by the device of the present invention, and then enters the mass spectrometer 4 for analysis. Such as image 3 As shown, the given mass spectrogram shows that protein molecules can be ionized under the non-contact AC voltage by using the present invention.

Embodiment 2

[0035]Implementation example 2: Select an ordinary glass tube as the medium to cover the outside of the output end of the capillary. The inner diameter of the medium is 5mm, the outer diameter is 7mm, and the length is 30mm. A 20mm wide tinfoil paper is attached to the outer wall of the medium and pasted into a circle to form a ring The electrode 2 and the ring electrode 2 are connected to the AC power supply 3, the other end of the AC power supply 3 is grounded, the peak-to-peak voltage range of the applied voltage is 10kV, and the frequency is 25kHz. The atomizer pressure was adjusted to 80psi. Sample solution: six mixed peptides were dissolved in methanol / water / acetic acid (8:2:0.1, v:v) solution, ionized by the present invention, and then entered into mass spectrometer 4 for analysis. The six peptides are:

[0036] A-Somatostatin, molecular weight 1638, CAS No. 38916-34-6

[0037] B-Dynorphin Acetate (1-13), molecular weight 1604, CAS No. 72957-38-1

[0038] C-alarelin ...

Embodiment 3

[0043] Implementation example 3: A copper wire loop with a length of 10mm and a diameter of 1mm is placed outside the capillary nozzle to form a ring electrode 2. The ring electrode 2 is connected to the AC power supply 3, and the other end of the AC power supply 3 is grounded. The peak-to-peak voltage range of the applied voltage is 3kV, and the frequency 50kHz. The atomizer pressure was adjusted to 80psi. Sample solution: dissolve hyrisalin acetate (molecular weight is 887) in methanol / water / acetic acid (8:2:0.1, v:v) solution, adopt traditional DC electrospray device and device of the present invention to carry out ionization respectively, then enter Mass spectrometer 4 for analysis. Such as Figure 5 As shown, the mass spectrum of hypersarin acetate is given. Figure 5 (a) is a traditional electrospray mass spectrogram, mainly including mass spectrum peaks with single charge and double charge and single charge Ghana mass spectrum peaks of hyrelsarin acetate; Figure 5 ...

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Abstract

The invention relates to a non-contact alternating current electrospray ionization device and a method. The device is characterized in that the device comprises a pneumatic spraying device, an electrode, an alternating current power supply and a mass spectrometer; the pneumatic spraying device comprises an external capillary tube, an internal capillary tube and a three-way connector; one end of the internal capillary tube is connected with a sample solution injection device, and the other end sequentially penetrates through a connector I and a connector II of the three-way connector; the external capillary tube is sleeved on the periphery of the internal capillary tube; one end of the external capillary tube is positioned in the three-way connector, and the other end is led out from the connector II of the three-way connector; a connector III of the three-way connector is connected with a high-pressure atomized gas storage device; the electrode is arranged on the periphery of outlet ends of the external capillary tube and the internal capillary tube and connected with the alternating current power supply; the internal capillary tube penetrates through the external capillary tube; and the mass spectrometer is arranged in front of the outlet ends of the external capillary tube and the internal capillary tube. The device has a simple structure, maintains characteristics of traditional electrospray, can be combined with high-efficiency liquid chromatography, capillary electrophoresis and microfluidics to be used for mass spectrographic analysis, and has a wide application prospect.

Description

technical field [0001] The invention relates to an electrospray ionization device and method, in particular to a non-contact AC electrospray ionization device and method for introducing liquid samples. Background technique [0002] Mass spectrometry is one of the powerful tools for qualitative and quantitative analysis of organic molecules. Electrospray ionization (ESI) is a typical ionization method in organic mass spectrometry. In the traditional electrospray process, the sample solution enters from one end of the capillary, and is sprayed out from the other end through high-pressure gas atomization. Under the action of atomizing gas and high-voltage electric field, the sample solution is atomized to form a charged aerosol. After solvent volatilization and Coulomb explosion, the target molecule is finally ionized and enters the mass spectrometer. The subsequently developed nano-electrospray ion source (nanoESI) eliminated the atomizing gas on the basis of the traditional...

Claims

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Application Information

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IPC IPC(8): H01J49/16G01N1/28
Inventor 张新荣彭月娥张四纯龚晓云马潇潇魏振威
Owner TSINGHUA UNIV
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