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Method for degrading phenol in waste water in coal chemical industry

A technology for coal chemical wastewater and phenol, which is used in chemical instruments and methods, water pollutants, biological water/sewage treatment, etc.

Inactive Publication Date: 2013-03-27
NINGXIA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no method for using domesticated Bacillus subtilis to degrade phenol in coal chemical industry wastewater

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for degrading phenol in coal chemical wastewater, the method comprising the steps of:

[0029] a. Seed preparation: pick a ring from the Bacillus subtilis preservation tube and insert it into the enrichment medium. After the strain is activated and expanded, inoculate the enrichment medium with an inoculum size of 5% by mass percentage. Aerobic cultivation at a temperature of 40°C for 24 hours can obtain degrading bacteria for domestication;

[0030] b. Acclimatization of degrading bacteria: Take the seeds prepared in the above step a and insert them into the basic selection medium containing 100mg / L phenol at an inoculum size of 5% by mass, at a temperature of 40°C and a stirring rate of 130r / min After 24 hours of aerobic culture, take the cultivated culture solution and inoculate it into the basic selection medium containing 200 mg / L phenol at an inoculum amount of 5% by mass, and culture it for 24 hours under the same conditions as above, and so on, that is,...

Embodiment 2

[0037] a. Seed preparation: pick a ring from the Bacillus subtilis preservation tube and insert it into the enrichment medium. After the strain is activated and expanded, it is inoculated into the enrichment medium with an inoculum size of 3% by mass percentage. Aerobic culture at 30°C for 48 hours to obtain degrading bacteria for domestication;

[0038] b. Acclimatization of degrading bacteria: Take the seeds prepared in the above step a and insert them into the basic selection medium containing 100 mg / L phenol at an inoculum size of 3% by mass, at a temperature of 30 °C and a stirring rate of 115 r / min After 48 hours of aerobic culture, take the cultivated culture solution and inoculate it into the basic selection medium containing 200mg / L phenol at an inoculation amount of 3% by mass percentage, and cultivate it under the same conditions as above for 48 hours, and so on, that is, take the former The culture solution after the first step of cultivation was inoculated into th...

Embodiment 3

[0045] a. Seed preparation: pick a ring from the Bacillus subtilis storage tube and insert it into the enrichment medium. After the strain is activated and expanded, it is inoculated into the enrichment medium with an inoculum of 1% by mass percentage. Aerobic culture at 20°C for 72 hours to obtain degrading bacteria for domestication;

[0046] b. Acclimatization of degrading bacteria: Take the seeds prepared in the above step a and insert them into the basic selection medium containing 100mg / L phenol at an inoculum size of 1% by mass, at a temperature of 20°C and a stirring rate of 100r / min After 72 hours of aerobic culture, take the cultivated culture solution and inoculate it into the basic selection medium containing 200 mg / L phenol at an inoculation amount of 1% by mass percentage, and cultivate it for 72 hours under the same conditions as above, and so on, that is, take the former The culture solution after the first step of cultivation was inoculated into the basic sele...

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Abstract

The invention relates to a method for degrading phenol in waste water in coal chemical industry, in particular to a method for degrading phenol in waste water in coal chemical industry by utilizing domesticated bacillus subtilis, which is characterized by including the following steps: a. preparing seed; b. domesticating degradation bacteria; c. separating single bacterial colony; d. culturing degradation phenol seed in amplifying mode; e. selecting phenol degradation bacteria; and f. introducing the phenol degradation bacteria selected in the step e. and receiving amplifying culture into the waste water in the coal chemical industry and culturing the phenol degradation bacteria for 24 to 72h under the temperature of 20-40 DEG C with the stirring speed as 100-130 r / min to degrade phenol in the waste water in the coal chemical industry. Compared with the prior art, the method can completely degrade phenol in waste water produced in coal preparation processes of carbinol, dimethyl ether and olefin, simultaneously has no secondary pollution to the environment and is unique in process.

Description

technical field [0001] The invention relates to a method for degrading phenol in coal chemical industry wastewater, in particular to a method for using domesticated Bacillus subtilis to degrade phenol in coal chemical industry wastewater. Background technique [0002] In the context of the increasingly urgent global demand for alternative chemical raw materials and alternative energy sources, China's coal chemical industry has become an important part of China's energy structure with its leading industrialization progress, and the state has also given more and more support to coal chemical companies. attention. At present, methanol, dimethyl ether, propylene, etc. are the main chemicals in the coal chemical industry chain. However, the presence of phenol in the wastewater produced by coal-to-methanol, coal-to-dimethyl ether, and coal-to-olefins greatly affects the treatment and disposal of coal chemical wastewater. [0003] Phenolic compounds are a kind of protoplasmic poi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34C02F101/34
Inventor 马玉龙任佳刘海艳
Owner NINGXIA UNIVERSITY
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