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Monoclonal antibody resistant to human phosphodiesterase 4D interaction protein variant 5 and application thereof

A technology of human phosphodiesterase and monoclonal antibody, applied in the direction of anti-animal/human immunoglobulin, microbial-based methods, biochemical equipment and methods, etc., can solve problems such as incomplete clarification of the relationship

Inactive Publication Date: 2013-07-17
TIANJIN TUMOR HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that the relationship between Myomegalin variants and tumors has not been fully elucidated and is worthy of further study

Method used

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  • Monoclonal antibody resistant to human phosphodiesterase 4D interaction protein variant 5 and application thereof
  • Monoclonal antibody resistant to human phosphodiesterase 4D interaction protein variant 5 and application thereof
  • Monoclonal antibody resistant to human phosphodiesterase 4D interaction protein variant 5 and application thereof

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Experimental program
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Embodiment Construction

[0018] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

[0019] 1. Preparation of monoclonal antibody against Myomegalin variant 5 (phosphodiesterase 4D interacting protein variant 5)

[0020] 1. Source of experimental materials

[0021] BALB / c mice were provided by Tianjin Cancer Hospital and purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.; Hela cells and mouse myeloma cells (SP2 / 0) were provided by Tianjin Cancer Hospital and purchased from Institute of Hematology, Chinese Academy of Sciences. Freund's complete adjuvant and incomplete Freund's adjuvant, HAT, and HT conditioned medium components were purchased from Invitrigen. DMEM medium, polyethylene glycol (PEG), and RPMI 1640 medium were purchased from Hyclone Company. Horseradish peroxidase (HRP)-labeled goat anti-mouse Ig, o-phenylenediamine (OPD), diaminobenzidine (DAB), and 8-azaguanine (8-AG) were purchased from Sig...

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Abstract

The invention discloses a monoclonal antibody resistant to a human phosphodiesterase 4D interaction protein variant 5 and an application of the monoclonal antibody, belonging to the preparation of antibody-containing medical preparations or monoclonal antibodies. The antibody specifically identifies 1103rd to 1116th amino acids of the human phosphodiesterase 4D interaction protein variant 5; the antibody is produced by a hybrid tumor cell strain with a collection number of CGMCC (China General Microbiological Culture Collection Center) No.5078, and has an amino acid sequence of GELESVRIHHKHAY. The monoclonal antibody has specific binding capacity with the phosphodiesterase 4D interaction protein variant 5, is used for detecting the expression and location of the phosphodiesterase 4D interaction protein variant 5 in human tissues and cells, and is used as an immunohistochemical detection reagent of the human phosphodiesterase 4D interaction protein variant 5.

Description

technical field [0001] The invention relates to the preparation of pharmaceutical preparations containing antibodies, or monoclonal antibodies, in particular to a monoclonal antibody against human phosphodiesterase 4D interacting protein variant 5 and its application. Background technique [0002] Myomegalin (MMG / MMGL / PDE4DIP, phosphodiesterase 4D interacting protein, phosphodiesterase 4D interacting protein) is a brand-new protein, which is distributed in the cytoplasm and gathers at the Golgi apparatus and centrosome, suggesting the molecular The main function involves cAMP signal transduction of these two organelles. Myomegalin gene is very long, about 150,000 base pairs, but each exon is very small, only about 30-40 amino acids, so there are many variants of Myomegalin. It is unclear exactly how many variants of Myomegalin exist. [0003] Myomegalin protein structure contains α helix and coiled-coil structure, as well as microtubule-associated protein binding domain an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/18C12N5/20G01N33/577C12R1/91
Inventor 张霖牛瑞芳罗艺杨毅魏熙胤齐众
Owner TIANJIN TUMOR HOSPITAL