Method for controlling heavy metals in process of culturing spirulina

A technology of spirulina and heavy metals, applied in the field of environmental engineering, can solve the problems of ineffective control of heavy metals, and achieve the effect of material safety, non-toxicity, low investment and easy operation

Active Publication Date: 2012-07-18
YUNNAN GREEN A BIOLOGICAL PROJECT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, the purpose of the present invention is to provide a method for controlling heavy metals in the spirulina cultivation process for the current problem that cannot effectively control the heavy

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1) Detect the content of heavy metals in the algae powder produced by the spirulina culture pond every 1-10 days, by graphite furnace atomic absorption spectrometry (see Chinese Pharmacopoeia 2010 edition first appendix IXB lead, cadmium, arsenic, mercury, copper determination method) detection; it was detected that the initial mass ratio content of Pb in the algae powder produced by the culture pond was 1.03ppm, and the initial mass ratio content of Cd was 0.61ppm.

[0023] 2) FeSO 4 ·7H 2 O is added to the spirulina culture medium of the culture pond by the mass volume ratio of 0.005g / L one day in advance and stirred for 1 day, then precipitated for 8 hours, and the heavy metal Pb and Cd were precipitated therein;

[0024] 3) Carry out a pond cleaning work every 40 days, and remove the sediment formed in the removal step 2) from the culture pond;

[0025] 4) Add EDTA-2Na to the spirulina culture medium in the culture pond at a mass-volume ratio of 0.005 g / L to compl...

Embodiment 2

[0031] 1) Detect once every 1-20 days the content of heavy metals in the algae powder produced by the spirulina culture pond, by graphite furnace atomic absorption spectrometry (see the first appendix IXB lead, cadmium, arsenic, mercury, copper determination method of Chinese Pharmacopoeia 2010 edition ) detection; it is detected that the initial mass ratio content of the algae powder Pb produced in the culture pond is 0.88ppm, and the initial mass ratio content of Cd is 0.12ppm.

[0032] 2) FeSO 4 ·7H 2 O was added to the spirulina culture medium of the culture pond by the mass volume ratio of 0.023g / L one day in advance and stirred for 1 day, then precipitated for 10 hours, and the heavy metal Pb and Cd were precipitated therein;

[0033] 3) Carry out a pond cleaning work every 40 days, and remove the sediment formed in the removal step 2) from the culture pond;

[0034] 4) Add EDTA-2Na to the spirulina culture medium in the culture pond at a mass volume ratio of 0.023g / L ...

Embodiment 3

[0039] 1) Detect once every 1-10 days the content of heavy metals in the algae powder produced by the spirulina culture pond, by graphite furnace atomic absorption spectrometry (see the Chinese Pharmacopoeia 2010 edition first appendix IXB lead, cadmium, arsenic, mercury, copper determination method ) detection; it is detected that the initial mass ratio content of the algae powder Pb produced in the culture pond is 2.06ppm, and the initial mass ratio content of Cd is 0.14ppm.

[0040] 2) FeSO 4 ·7H 2 O was added to the spirulina culture medium of the culture pond by the mass volume ratio of 0.05g / L one day in advance and stirred for 1 day, then precipitated for 12 hours, and the heavy metal Pb and Cd were precipitated therein;

[0041] 3) Carry out a pond cleaning work every 40 days, and remove the sediment formed in the removal step 2) from the culture pond;

[0042] 4) Then add EDTA-2Na into the spirulina culture medium in the culture pond at a mass volume ratio of 0.05g / ...

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Abstract

The invention provides a method for controlling heavy metals in the process of culturing spirulina, which comprises the following steps of: detecting the content of the heavy metals in algae powder produced in a culture medium of a spirulina culture pond; when the mass ratio content of Pb in the algae powder in the spirulina culture is over 0.88ppm or the mass ratio content of Cd in the algae powder in the spirulina culture is over 0.12ppm, adding FeSO4-7H2O into the culture medium of the spirulina culture pond and precipitating the heavy metals Pb and Cd in the culture medium; cleaning the pond, i.e. pouring out formed precipitates; and adding EDTA-2Na into the culture medium of the spirulina culture pond and complexing the heavy metals to precipitate the heavy metals. FeSO4.7H2O used inthe method can provide iron ions for the spirulina; after FeSO4.7H2O is added into the culture medium, the heavy metal ion sedimentation can be accelerated; and due to the adoption of the steps of cleaning the pond and adding the EDTa-2Na complex, the heavy metals entering a culture system from the outside environment are reduced, the spirulina is safe and nontoxic, the culture medium can be repeatedly used and the method is easy to operate.

Description

technical field [0001] The invention relates to a method for controlling heavy metals, in particular to a method for controlling heavy metals in the cultivation process of spirulina, and belongs to the technical field of environmental engineering. Background technique [0002] Spirulina (spirulina) belongs to Cyanophyta (Cyanophyta), Oscillatoriaceae, Oscillatoriaceae, Spirulina genus. It is a filament composed of unicellular or multicellular, without sheath, cylindrical, loose or tight regular helical bending. Its growth attributes are light-loving, alkali-loving, and high-temperature-loving. Spirulina has been valued and concerned by many countries and international organizations for its high nutritional value and excellent medical and health effects. At present, spirulina products have entered the fields of food, health food and medicine to serve the health of the public. [0003] Since spirulina adopts factory farming, it is inevitably affected by factors such as bree...

Claims

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Application Information

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IPC IPC(8): C12Q3/00
Inventor 吕荣祥鸭乔杜伟春张瑶
Owner YUNNAN GREEN A BIOLOGICAL PROJECT
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