Preparation method of madrepore skeleton sample for being suitable for observing skeleton minute structure and researching environment record of madrepore

Abstract

The present invention relates to a method for preparing a stony coral bone sample applicable to observing the microstructure of the skeleton and studying its environmental record, comprising the following steps: (1) soaking the stony coral bone sample in bleaching solution for the first time, and washing it with clean water , and then soak for the second time in a new bleaching solution; the bleaching solution is 10% hydrogen peroxide or NaClO solution with a pH of 8-9; (2) after the immersion, the stony coral skeleton sample is further cleaned, and then Dry at 45°C~60°C for later use. The present invention removes coral polyp tissue and other organic impurities by using common reagents such as hydrogen peroxide and an ultrasonic cleaner, thereby obtaining clean stony coral skeletons, minimizing the damage to coral skeleton structures and microstructures in the preparation process, and avoiding the destruction of the skeleton Changes in trace element content and isotopic composition ratios.

Description

technical field [0001] The invention belongs to the fields of biogeochemistry and environmental ecology, and relates to a method for preparing a reef-building coral bone sample for experiments. Background technique [0002] Reef-building corals secrete and deposit calcium carbonate during life activities, forming a continuous layer of skeletal growth. Reef-building corals are sensitive to changes in the external environment. Diurnal changes in environmental factors such as temperature, salinity, and pH will cause changes in the physiological status and even health of coral polyps. The biochemical properties of aragonite formed by calcification during the same period will also be affected. Influence, such as the isotope δ contained in it 18 O, δ 13 C, delta 11 Changes in the B composition ratio, changes in the content of geochemical elements (including heavy metal elements), changes in bone density bands, and so on. Therefore, the growth of reef-building coral skeletons s...

AI Technical Summary

Problems solved by technology

However, the descriptions of these three methods are not very detailed, and there is no systematic discussion and introduction on how to effectively remove impurities inside coral tissues and bones without affecting the fine structure and composition of bones

In fact, NaClO has the strongest oxidizing ability, but its toxicity and secondary pollution caused by oxidation are inevitable; while NaOH has excellent corrosiveness, but it will also cause secondary pollution and interfere with the observation of fine structures. The post-cleaning can eliminate the above-mentioned effects

Due to the particularity of its chemical composition, hydrogen peroxide can oxidize and decontaminate while basically causing no secondary pollution. However, because of its water solubility and weak acidity, it will lead to the dissolution of microscopic structures such as aragonite needle crystals.