Method for determining hydrogen peroxide content
A hydrogen peroxide, content technology, used in material excitation analysis, fluorescence/phosphorescence, etc., to achieve high sensitivity and good selectivity
Inactive Publication Date: 2012-07-18
GUILIN UNIVERSITY OF TECHNOLOGY
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Tetracarboxyiron phthalocyanine (FeC4Pc) can be used as a peroxide mimetic enzyme, but there is no report on the method for the determination of hydrogen peroxide content by the fluorescence quenching method of rhodamine B catalyzed by tetracarboxyferrophthalocyanine
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[0012] (1) Add 0.0, 0.02, 0.05, 0.1, 0.2, 0.5, 1.0, 1.3, 1.4, 1.5mL 1.624×10 to ten 5.0mL colorimetric tubes -5 mol / L of H 2 o 2 , and then add 0.4mL5×10 to each colorimetric tube -6 mol / L rhodamine B solution, 0.9mL 1.0×10 -4 mol / L of tetracarboxyiron phthalocyanine, without adding H2O2 as reagent blank;
[0013] (2) The solutions in the 10 colorimetric tubes obtained in step (1) were all diluted to the 5.0mL scale with ultrapure water, shaken up, reacted for 80min, and then the Use a 1cm quartz cuvette, set the excitation wavelength to 554nm, the emission wavelength to 575nm, the excitation and emission slits are both 5nm, the voltage is 600V, respectively measure the H 2 o 2 The fluorescence value of the solution F and without H 2 o 2 The fluorescence value of the reagent blank F 0 , calculate the difference ΔF=F 0 -F, the linear regression equation of its fluorescence difference ΔF and hydrogen peroxide concentration c is ΔF=8.646×10 7 c+14.30, correlation coeffi...
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The invention discloses an analysis method for determining hydrogen peroxide content. 0.0mL, 0.02mL, 0.05mL, 0.1mL, 0.2mL, 0.5mL, 1.0mL, 1.3mL, 1.4mL and 1.5mL of 0.5 to 2.5 multiplied by 10 negative 5mol / L H2O2 are sequentially added into ten 5.0mL color comparison tubes; 0.1mL to 0.6mL of 3.5 to 6.5 multiplied by 10 negative 6mol / L rhodamine B solution and 0.3mL to 1.5mL of 0.5 to 2.0 multiplied by 10 negative 4mol / L tetracarboxy iron phthalocynine are then respectively added into each color comparison tube; reagent blank is not added with H2O2; the solutions in the ten color comparison tubes are respectively diluted to the 5.0mL graduation by ultra-pure water, uniformly agitated and react for 70 to 90 minutes; the fluorescence value F of the solutions added with H2O2 and the fluorescence value F0 of the reagent blank without H2O2 are then respectively determined by a 1cm quartz cuvette on a fluophotometer, wherein excitation wavelength is set at 554nm, emission wavelength is set at 575nm, both an excitation slit and an emission slit are set at 5nm, voltage is set at 600V, and the difference (Delta F equal to F0 minus F) is calculated; and in addition, 0.5 to 2.0mL of rainwater is collected, and the fluorescence value of the rainwater is determined by the same method. The determination method is highly sensitive, highly selective, simple and convenient.
Description
technical field [0001] The invention relates to an analysis method for determining the content of hydrogen peroxide, in particular to a method for determining the content of hydrogen peroxide by a fluorescent quenching method for catalyzing the oxidation of rhodamine B by tetracarboxy iron phthalocyanine. Background technique [0002] Hydrogen peroxide is a commonly used chemical reagent, which can be used as a disinfectant, bactericide, deoxidizer, bleach, initiator of polymerization reaction, etc. In recent years, hydrogen peroxide also widely exists in nature, and is one of the important reasons for the formation of acid rain, so the determination of hydrogen peroxide is of great significance for environmental monitoring and clinical analysis. At present, the methods for determining hydrogen peroxide mainly include titration, spectrophotometry, electrochemical method, chemiluminescence method, chromatography, resonance scattering spectrometry, fluorescence photometry, ato...
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IPC IPC(8): G01N21/64
Inventor 唐宁莉蒙兴龙凌悦菲李武生
Owner GUILIN UNIVERSITY OF TECHNOLOGY