Domestic silkworm cuticle protein BmCP231 promoter as well as recombinant expression vector and application thereof

An epidermal protein and expression vector technology, applied in recombinant DNA technology, the use of vectors to introduce foreign genetic material, DNA/RNA fragments, etc., can solve the problems that have not been fully elucidated, mechanical properties cannot be compared with spider silk fibers, etc.

Active Publication Date: 2012-07-25
SOUTHWEST UNIVERSITY
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  • Abstract
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AI Technical Summary

Problems solved by technology

The formation of silk fibers in living organisms is an extremely complex process that has not been fully elucidated so far
Existing synthetic fibers are artificially si

Method used

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  • Domestic silkworm cuticle protein BmCP231 promoter as well as recombinant expression vector and application thereof
  • Domestic silkworm cuticle protein BmCP231 promoter as well as recombinant expression vector and application thereof
  • Domestic silkworm cuticle protein BmCP231 promoter as well as recombinant expression vector and application thereof

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Experimental program
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Embodiment Construction

[0015] In order to make the purpose, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings. For the experimental methods not specified in the examples, the conventional conditions are generally followed, such as the conditions described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al.), or the conditions suggested by the manufacturer.

[0016] The silkworm species used in the examples is Dazao, provided by the Silkworm Gene Resource Bank of Southwest University of China.

[0017] 1. Cloning of Bombyx mori epidermal protein BmCP231 promoter

[0018] According to the sequence nscaf2986 in the silkworm genome database, two pairs of upstream and downstream primers were designed respectively. The designed primers were synthesized by Sangon Bioengineering (Shanghai) Co., Ltd. The primer sequences are as follows:

[0019] 231-F: 5'...

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Abstract

The invention discloses a domestic silkworm cuticle protein BmCP231 promoter which is formed by No.1 nucleotide to No.1 1457 nucleotide in the SEQ ID No. 1 or No.1 nucleotide to No.1 1511 nucleotide in the SEQ ID No. 1. The domestic silkworm cuticle protein BmCP231 promoter can drive the specific expression of the exogenous protein on the front silk gland of the domestic silkworm and can be used as the specific promoter of the front silk gland of the domestic silkworm. The invention further discloses a recombinant expression vector containing the domestic silkworm cuticle protein BmCP231 promoter. A powerful tool is provided for the next-step research on regulating the expression of the protein of the ion channel for the front silk gland of the domestic silkworm to affect the spinning behavior of the domestic silkworm and improve the silk fiber performance.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a tissue-specific promoter, its expression vector and its application. Background technique [0002] The silk fiber produced by the silkworm under natural conditions is a fiber material widely used in textile industry, biomedicine and other fields. Spiders and silkworms have similar spinning bodies, but the silk fibers produced by them have stronger hardness and better toughness than silkworm silk. The formation of silk fibers in living organisms is an extremely complicated process, which has not been fully elucidated so far. Existing synthetic fibers are artificially simulated in the natural silk production process of insects and synthesized in vitro, but their mechanical properties are far from being comparable to those of spider silk fibers. If the silkworm can be efficiently and specifically produced a large amount of high-quality spider silk-like fibers, it will be of great sign...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12N15/85
Inventor 夏庆友衣启营赵萍王鑫马三垣王峰
Owner SOUTHWEST UNIVERSITY
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