Integrated device for fumigating, freezing, storing and transporting gametes based on liquid nitrogen

Abstract

An integrated device for fumigating, freezing, storing and transporting gametes based on ultra-low temperature liquid nitrogen comprises a movable trolley, a primary liquid nitrogen storage tank, a storage tank thermal insulation cover, a liquid nitrogen recovery tank, a low-temperature valve, a gamete carrier frame and a temperature feedback device. The primary liquid nitrogen storage tank adopts polyurethane insulation materials or vacuum multilayer insulation to keep warm, is provided with a polyurethane insulation cover, and can achieve that the outer surface of a storage tank body does not frost and dew. The primary liquid nitrogen storage tank is design in a form that a cushion chamber and an operating chamber are separated to form a communicating vessel, stability of a liquid level and the temperature of the operating chamber is guaranteed, and serious fluctuation caused in a liquid adding process is avoided. Temperature measuring and displaying functions are provided, and temperature information is used for adjusting the height of the liquid level simultaneously. The integrated device can achieve single-step fumigation and safety transportation of the gametes.

Description

technical field [0001] The invention relates to a medical gamete freezing storage and transportation device, in particular to an integrated device for liquid nitrogen fumigation freezing and gamete storage and transportation. technical background [0002] Gamete (sperm / egg) health is closely related to human race reproduction and population quality. Using ultra-low temperature freezing and thawing technology to preserve gamete embryos and produce offspring through assisted reproductive technology has become a hot spot in reproductive medicine and has been highly concerned. At present, there are several defects in the "slow freezing and quick thawing" of sperm and vitrification of egg embryos in China: [0003] First, in the process of transfer to the low-temperature storage container after the professional program cooling device completes the freezing, there is no professional storage and transportation device, and most gametes are transported through ordinary foam boxes, w...

AI Technical Summary

Problems solved by technology

[0003] First, in the process of transfer to the low-temperature storage container after the professional program cooling device completes the freezing, there is no professional storage and transportation device, and most gametes are transported through ordinary foam boxes, which not only easily causes damage to the rewarming of the gametes, but also causes frostbite of transport personnel Hidden danger

[0004] Second, during the storage and transportation process, different cryopreservation methods of various gamete samples in different carriers were not considered, such as immersion in liquid nitrogen or fumigation, which may easily cause damage to some gametes by ice crystals inside and outside the cells, resulting in a low recovery success rate

[0005] Third, the reusability of liquid nitrogen used for freezing gametes is not considered. After the gametes are transported once, the liquid nitrogen volatilizes, resulting in a waste of resources.

[0006] Fourth, the biological safety of samples during the freezing process is not considered, that is, the preserved gamete embryo samples may be contaminated by liquid nitrogen and may cross-infect group samples

The device can preserve gametes, but its main defect is that the number of preserved gametes is small, the time is short, and it only has a single freezing function, and it cannot fumigate and freeze a large number of independent gametes at the same time

[0010] Further searches found that the Chinese patent "Cryogenic Preservation of Sperm" with the authorized announcement number CN1450857 stored sperm at three different temperatures, but it did not involve the transportation and transfer of preserved sperm

Images