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Method for detecting 1,8-diaminonaphthalene based on optical DNA (Deoxyribonucleic Acid) biosensor

A biosensor, diaminonaphthalene technology, applied in the fields of analytical chemistry and DNA biosensing, can solve the problems of long electrode preparation time, increased response speed, shortened cycle, etc., and achieves short experimental period, fast response speed and high reliability. Effect

Inactive Publication Date: 2012-08-01
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the detection of aromatic amine compounds using DNA as the main body of molecular recognition mostly uses DNA-modified electrode sensors. Although these DNA biosensors have high sensitivity to aromatic amine pollutants, the disadvantage is that the electrode preparation Long time, relatively high cost
So far, the application of G-DNA catalyzed luminol chemiluminescence to the detection of aromatic amines has not been reported. Compared with the DNA-modified solid electrode sensor method, this method does not need to modify the DNA on the electrode surface, greatly shortening the time. The cycle time is reduced, and the cost is reduced, and the response speed of detection is also significantly improved

Method used

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  • Method for detecting 1,8-diaminonaphthalene based on optical DNA (Deoxyribonucleic Acid) biosensor
  • Method for detecting 1,8-diaminonaphthalene based on optical DNA (Deoxyribonucleic Acid) biosensor
  • Method for detecting 1,8-diaminonaphthalene based on optical DNA (Deoxyribonucleic Acid) biosensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) 20 mM Tris-NaClO at pH=8.0 4 Dissolve the solid hairpin DNA in the buffer solution, mix it with a vortex shaker, and let it stand at room temperature for 6 hours. Under this condition, the hairpin DNA structure is formed;

[0028] 2) Take an appropriate amount of the hairpinDNA solution prepared in the above step 1) and add it to the cryopreservation tube, and add a certain concentration of 1,8-diaminonaphthalene solution to it, let it stand for 30 minutes, and then add a certain concentration of K to the above system + And hemin solution, placed for 1h, under this condition, a G-DNA structure with peroxidase-like activity was formed;

Embodiment 2

[0030] Control assay system hairpin DNA concentration is 100nM, K + Concentration is 10mM, hemin concentration is 1μM, H 2 o 2 Concentration of 300μM, luminol concentration of 50μM, respectively, with 20mM Tris-NaClO 4 1,8-diaminonaphthalene (6nmol / L, 30nmol / L, 150nmol / L, 300nmol / L and 600nmol / L) solutions with different concentrations prepared in a buffer solution (pH=8.0) were used to measure the chemiluminescent intensity of the system. The above-mentioned 1,8-diaminonaphthalene solutions with different concentrations were subjected to chemiluminescence experiments, and the chemiluminescence intensities are shown in Table 1.

[0031] Table 1 Chemiluminescence intensity after interacting with different concentrations of 1,8-diaminonaphthalene

[0032]

Embodiment 3

[0034] The concentration of 50nM hairpin DNA was used to replace the concentration of 100nM hairpin DNA in Example 2, and other conditions were the same as in Example 2. Experiments showed that this optical DNA biosensor also responded to 1,8-diaminonaphthalene. See Table 2 for the chemiluminescence intensities of different concentrations of 1,8-diaminonaphthalene solutions under these conditions.

[0035] Table 1 Chemiluminescence intensity after interacting with different concentrations of 1,8-diaminonaphthalene

[0036]

[0037]

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Abstract

The invention discloses a method for detecting 1,8-diaminonaphthalene based on an optical DNA (Deoxyribonucleic Acid) biosensor, which belongs to the technical field of analytical chemistry detection and DNA biosensors, and relates to detection of 1,8-diaminonaphthalene on the basis of catalyzing oxidation of luminol with hydrogen peroxide for generating chemiluminescence by using a peroxydase-like G-DNA. The method comprises the following specific step of: making a hairpin DNA containing a G-DNA structure and serving as a raw material react with 1,8-diaminonaphthalene to form a G-DNA with peroxydase-like catalytic activity under the actions of K<+> and hemin,; and detection of the 1,8-diaminonaphthalene through the change of chemiluminescence generated by the luminol oxidized with the hydrogen peroxide is realized. The optical DNA biosensor method has the advantages of easiness, convenience, short detection period, quick response, high stability, high sensitivity, and the like.

Description

technical field [0001] The invention belongs to the technical fields of analytical chemistry and DNA biosensing, and relates to a detection method of 1,8-diaminonaphthalene based on an optical DNA biosensor, which can quickly and effectively detect 1,8-diaminonaphthalene. Background technique [0002] Aromatic amines are an important class of environmental pollutants and have been listed as one of the priority environmental pollutants for monitoring. Aromatic amines are widely used in industry. As important chemical raw materials and fine chemical intermediates, they are often used in the production processes of printing and dyeing, pharmaceuticals, medicine, pesticides, plastics, rubber, textiles, ceramic glazing and paints, etc., especially In the printing and dyeing industry, wastewater often contains high concentrations of aromatic amine intermediates, and some dyes will eventually release aromatic amine pollutants through complex chemical reactions and microbial actions...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76
Inventor 刘新会梁刚李晓宏巩文雯成登苗陶莉刘冠男
Owner BEIJING NORMAL UNIVERSITY