Method of preparing capsaicine
A technology for capsaicin and capsaicin, which is applied in the field of preparation of capsaicin, can solve the problems of high production environment and equipment requirements, complicated extraction process, unfavorable industrialized production and the like, and achieves low equipment requirements, no environmental pollution, good quality The effect of adsorption
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Embodiment 1
[0022] Take capsicum essence 0.3kg and transfer to a separatory funnel, add 1.5L of ethyl acetate to dissolve it, add 75g of sodium carbonate and 15g of sodium chloride into 1.5L of purified water to dissolve the prepared mixed lye for extraction, let stand for 12 hours, the ethyl acetate layer was concentrated and evaporated to dryness to obtain an extract oil.
[0023] The extracted oil was dissolved in 50% ethanol and subjected to polyamide column chromatography with 50% ethanol as the eluent. The eluate rich in capsaicin was collected, concentrated, and evaporated to dryness to obtain column chromatography oil.
[0024] The column chromatography oil was weighed, dissolved with 0.4 times the weight of the column chromatography oil and n-hexane-ethyl acetate with a volume ratio of 20:80, transferred to a -10°C freezer for 24 hours, and suction filtered to obtain the needle crystalline product. The content of capsaicin detected by HPLC is 94.9%, and the transfer rate of caps...
Embodiment 2
[0026] Take capsicum essence 0.5kg and transfer to a separatory funnel, add ethyl acetate 2.5L to make it dissolve, add 125g sodium carbonate and 25g sodium chloride to 2.5L purified water and dissolve the mixed lye prepared for extraction, let stand for 12 hours, the ethyl acetate layer was concentrated and evaporated to dryness to obtain an extract oil.
[0027] The extracted oil was dissolved in 60% ethanol and subjected to polyamide column chromatography with 60% ethanol as the eluent. The eluate rich in capsaicin was collected, concentrated, and evaporated to dryness to obtain column chromatography oil.
[0028] The column chromatography oil was weighed, dissolved with 0.4 times the weight of the column chromatography oil and n-hexane-ethyl acetate with a volume ratio of 30:70, transferred to a -12°C freezer for 24 hours, and suction filtered to obtain the needle crystalline product. The content of capsaicin detected by HPLC is 93.5%, and the transfer rate of capsaicin i...
Embodiment 3
[0030] Weigh 1 kg of capsicum essence and transfer it to a multifunctional glass reaction kettle, add 5 L of ethyl acetate to dissolve it, add 300 g of sodium carbonate and 50 g of sodium chloride to 5 L of purified water to dissolve the mixed lye for extraction, and let it stand for 12 hours , the ethyl acetate layer was concentrated and evaporated to dryness to obtain an extract oil.
[0031] The extracted oil was dissolved in 60% ethanol and subjected to polyamide column chromatography with 60% ethanol as the eluent. The eluate rich in capsaicin was collected, concentrated, and evaporated to dryness to obtain column chromatography oil.
[0032] The column chromatography oil was weighed, dissolved in n-hexane-ethyl acetate with a volume ratio of 40:60 and 1 times the weight of the column chromatography oil, transferred to a -15°C freezer for 30 hours, and suction filtered to obtain the needle crystalline product. The content of capsaicin detected by HPLC is 95.2%, and the t...
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