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Application of HVEM (herpesvirus entry mediator) gene in preparing product for liver cancer diagnosis and prognosis prediction

A gene, liver cancer technology, applied in the application field of HVEM gene, to achieve the effect of accurate diagnosis and prognosis prediction

Inactive Publication Date: 2014-06-04
HEPATOBILIARY SURGERY HOSPITAL SECOND MILITARY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research and clinical significance of HVEM in solid tumors, and the mechanism of HVEM in anti-tumor have not been reported yet.

Method used

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  • Application of HVEM (herpesvirus entry mediator) gene in preparing product for liver cancer diagnosis and prognosis prediction
  • Application of HVEM (herpesvirus entry mediator) gene in preparing product for liver cancer diagnosis and prognosis prediction
  • Application of HVEM (herpesvirus entry mediator) gene in preparing product for liver cancer diagnosis and prognosis prediction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Expression of HVEM in liver cancer and adjacent tumors

[0030] 1. Materials and methods

[0031] 1.1 Experimental materials

[0032] 1.1.1 Tissue samples

[0033] Tissue sources for immunohistochemistry: 103 cases of liver cancer (HCC) and corresponding paracancerous tissue paraffin sections were obtained from the specimens of radical resection of liver cancer from January 2004 to June 2004 in the Department of Pathology, Eastern Hepatobiliary Surgery Hospital.

[0034] Tissue sources for Realtime-PCR and Western-blot: fresh tissue samples from radical resection of liver cancer in Eastern Hepatobiliary Surgery Hospital from January 2009 to June 2009.

[0035] 1.1.2 Main reagents

[0036] ⑴ Reagents for immunohistochemistry:

[0037] Primary antibody (used concentration 1:200), HVEM (N-19, SC-7766), Stanta Company; antibody diluent (Long Island Company); secondary antibody (used concentration 1:200), HRP-labeled rabbit anti-goat IgG, ICL company; dilute...

Embodiment 2

[0093] Example 2 The effect of HVEM on the growth and metastasis of liver cancer cells

[0094] 1. Materials and methods

[0095] 1.1 Materials

[0096] Cell lines: Human liver cancer cell lines SMMC-7721, HepG2, Hep3B and stem cell lines Chang and HL-7702 are preserved by our laboratory.

[0097] Cell culture reagents: newborn calf serum (NCS), fetal bovine serum (FBS), DMEM medium and trypsin were purchased from Gibco.

[0098] Three-gas incubator: The N2-O2-CO2 incubator (MCO-18M) for hypoxic culture is a product of Japan's SANYO company.

[0099] High-purity nitrogen (N2): N2 (≥99.999%) was purchased from Shanghai Chenggong Gas Industry Co., Ltd.

[0100] Kits: QIAprepR Miniprep Kit was purchased from Qiagen; the Annexin V-FITC Cell Apoptosis Detection Kit was purchased from Nanjing Kaiji Biotechnology Development Co., Ltd.; the transfection reagent FuGENER HD Transfection Reagent was purchased from Roche .

[0101] Bacterial culture reagents: tryptone, yeast extrac...

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Abstract

The invention relates to application of an HVEM (herpesvirus entry mediator) gene in preparing a product for liver cancer diagnosis and prognosis prediction. The invention also provides application of the HVEM gene in preparing a product for predicting liver cancer prognosis and application of the HVEM gene in preparing a medicament for treating liver cancer. The application disclosed by the invention has the advantages that the abnormal expression of HVEM in a solid tumor tissue is discovered for the first time; the HVEM gene can be used as a specific marker gene for diagnosing the liver cancer and predicting the liver cancer prognosis so that the liver cancer diagnosis and the prognosis prediction are more accurate and quicker; and a new treatment target and a new effective medicament are provided for preventing and treating the liver cancer.

Description

technical field [0001] The invention relates to an application of the HVEM gene, in particular to the application of the HVEM gene in preparing products for diagnosis and prognosis prediction of liver cancer. Background technique [0002] Hepatocellular carcinoma (HCC) is one of the most common malignant tumors in my country. It is currently the second leading cause of cancer-related death in my country, and is gradually increasing, seriously endangering the health of our people. The treatment of liver cancer is still a comprehensive treatment based on surgery, but patients with advanced stage often lose the chance of surgery. Liver cancer has a high degree of malignancy, rapid development, and easy recurrence, so the overall curative effect is still unsatisfactory, and the annual death toll is almost equal to the number of new cases. The idea of ​​harnessing the immune system to attack liver cancer has been proposed for a long time, but has so far failed to produce the des...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68G01N33/574A61K38/17A61P35/00
Inventor 夏勇沈锋李俊
Owner HEPATOBILIARY SURGERY HOSPITAL SECOND MILITARY MEDICAL UNIV
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