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Method for changing ingredients of plant secondary metabolites by using beta-ocimene

A technology of secondary metabolites and ocimene, applied in botany equipment and methods, horticultural methods, applications, etc., can solve problems such as changing the composition of plant secondary metabolites

Active Publication Date: 2013-01-23
HUNAN AGRICULTURAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far there is no literature or patent report about the use of ocimene to change the composition of plant secondary metabolites

Method used

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  • Method for changing ingredients of plant secondary metabolites by using beta-ocimene
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  • Method for changing ingredients of plant secondary metabolites by using beta-ocimene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1: Alteration of secondary metabolite components of Echinacea purpurea with β-ocimene

[0013] 1. Processing of plant material

[0014] 1. Put two Echinacea purpurea plants that have grown for 30 days into two desiccators that have been cleaned and air-dried;

[0015] 2. Add β-ocimene to one of the desiccators so that the final concentration of β-ocimene in the desiccator is 10 μmol / L, and quickly cover the lid as the treatment group; add nothing to the other desiccator as the treatment group. Blank control;

[0016] 3. Seal the two desiccators, and then put them into a plant growth room with a temperature of 20-25°C, a light of 8000-10000 Lux, and a relative humidity of 60-75%, and treat them for 3 days.

[0017] 2. Extraction of secondary metabolites in Echinacea purpurea (using conventional solvent extraction technology)

[0018] 1. Wash the two cultured Echinacea purpurea (including the roots), place them in a mortar respectively, add twice the weight of ...

example 2

[0023] Example 2: β-Ocimene Alters the Composition of Volatiles in Tomato Plants

[0024] 1) Treatment of plant material:

[0025] 1. Put two 25-day-old tomato seedlings into two clean and air-dried desiccators respectively;

[0026] 2. Add β-ocimene to one of the desiccators, and quickly cover the lid so that the final concentration of β-ocimene in the desiccator is 5 μmol / L, as the treatment group; add nothing to the other desiccator, as the treatment group. Blank control;

[0027] 3. Seal the two desiccators, then put them into a plant growth room with a temperature of 20-25°C, a light of 8000-10000 Lux, and a relative humidity of 60-75%, and treat for 24 hours.

[0028] 2) Take the tomato seedlings of the control group and the treatment group out of the desiccator, transfer them to the volatile matter collector respectively, and collect the volatile products of tomato seedlings with two different treatments.

[0029] 3) Determination of volatiles by gas chromatography (...

example 3

[0031] Example 3: glucosinolate content in rapeseed treated with β-ocimene

[0032] 1) Rapeseed treatment: 10 plants of Brassica napus GX-27 were planted in each of two 5-square-meter artificial climate chambers, and β-ocimene was added to one chamber to a final concentration of 5 μmol / L as a treatment group; the other One chamber without β-ocimene was used as a control group. The conditions of the artificial climate chamber are: the temperature is 20-25°C, the light is 10000-20000Lux, and the relative humidity is 50-65%.

[0033] 2) Determination of total glucosinolate content: After the rapeseeds were harvested by individual plants, the glucosinolate content in the rapeseed was measured by near-infrared spectroscopy. was 103.24 μmol / g, and the difference between the two reached an extremely significant level, as shown in Table 1 below. It shows that β-ocimene treatment can increase the content of secondary metabolite glucosinolate in rapeseed.

[0034] Table 1

[0035] ...

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Abstract

The invention discloses a method for changing the ingredients of plant secondary metabolites by using beta-ocimene, comprising the following steps of: planting or transferring plants or plant tissue cultures in a closed space of which temperature, humidity and light conditions suitable for the growth of the plants or the plant tissue cultures, adding beta-ocimene in the space till the final concentration of the beta-ocimene in the space is 3-40 mu mol / L, and continuously treating the plants or the plant tissue cultures for 1-10 days, wherein the other conditions during the treatment, such as temperature, humidity and light, are suitable for the growth of the plants or the plant tissue cultures, and the specific conditions are different for the different types of plants. Therefore, the constituents and contents of the plant secondary metabolites can be changed through the beta-ocimene, and a new way is provided for obtaining the effective functional ingredients of plants or increasing the contents of the functional ingredients of the plants.

Description

Technical field: [0001] The invention relates to a method for changing the composition of plant secondary metabolites, in particular to a method for changing the composition of plant secondary metabolites by using β-ocimene. Background technique: [0002] The concept of plant secondary metabolism was first clearly proposed by Kossel in 1891. Substances produced by plants through secondary metabolic pathways can be called secondary metabolites. Secondary metabolites can be divided into seven categories: phenylpropanoids, quinones, flavonoids, tannins, terpenes, steroids and their glycosides, and alkaloids. There are also people who divide secondary products into three categories, phenolic compounds, terpenoids, and nitrogen-containing compounds (such as alkaloids) according to their biological sources. Secondary metabolism is an important life activity of plants, which is closely related to the growth and development of plants and their adaptation to the environment. At th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G7/06
Inventor 刘春林阮颖肖牧
Owner HUNAN AGRICULTURAL UNIV